產(chǎn)氣莢膜梭菌α毒素基因在干酪乳桿菌基因組中的表達及免疫效果評價.pdf

1、ClassifiedIndexQ93994Confidential(yes／no)：NoCode：10224NO：110034DissertationfortheDoctoralDegreeTheExpressionofAlphaToxinGeneofClostridiumPerfringensinLcaseiGenomeandAssessmentofImmuneEffectCandidate：BaifenSongSuIYijiIngL

2、iupervasor：Y11DegreeCategory：DoctorofAgricultureCollege：CollegeofVeterinaryMedicineFirstleveldiscipline：VeterinaryMedicineSecondleveldiscipline：PreventiveVeterinaryMedicineHarbinChinaJune2014東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)博士學(xué)位論文35表達盒在pPG612載體中

3、的表達驗證3936pGBHCupp2A2BPPaT重組質(zhì)粒的獲得一39361pGBHCupp2A2BPPaT／TGl質(zhì)粒酶切鑒定結(jié)果39362pGBHCupp2A2BPPaT／Lcasei質(zhì)粒鑒定結(jié)果34363pGBHCupp2A2BPPaT重組質(zhì)粒構(gòu)建策略3537pGBHCupp2A2BPPctT質(zhì)粒與Lcasei重組鑒定結(jié)果37371PCR鑒定結(jié)果37372基因組PCR測序結(jié)果3738PPaTAupp三casei重組菌特性研究383

4、81PPctTtIuppLcasei形態(tài)學(xué)特性分析38382PPctTAuppLcasei遺傳穩(wěn)定性分析38383PPaTAuppLcasei生長特性分析393845FU抗性研究4039PPaTAupp三casei基因組表達結(jié)果41391Westernblotting結(jié)果4l392間接ELISA結(jié)果48393激光共聚焦結(jié)果。48310El服PPctTzxuppLcaseiATCC免疫學(xué)指標的測定一483101免疫小鼠特異性抗體IgA測定

5、483102免疫小鼠血清中特異性抗體IgG測定453103脾淋巴細胞增殖實驗463104CD4細胞亞類及細胞因子的檢測47311免疫小鼠攻毒保護性試驗結(jié)果503111天然(It毒素致病性試驗結(jié)果一503112最小致死量(MLD)測定結(jié)果513113攻毒試驗結(jié)果513114免疫小鼠血清對產(chǎn)氣莢膜梭菌a毒素的中和效力試驗結(jié)果514討論一5341重組產(chǎn)氣莢膜梭菌a毒素的去毒性5342構(gòu)建的表達盒元件5343溫敏型質(zhì)粒和LcaseiATCC39