重組人粒細胞集落刺激因子對HL-60細胞的作用及其對阿糖胞苷毒性作用影響的研究.pdf

1、青島大學碩士學位論文重組人粒細胞集落刺激因子對HL60細胞的作用及其對阿糖胞苷毒性作用影響的研究姓名：王（钅厷)申請學位級別：碩士專業(yè)：兒內(nèi)科（小兒血液）指導教師：孫立榮20050528英文摘要STUDYoNTHEEFFECTSoFHUMANRECoMBINANTGRANULOCYTECLoNYSTUMILATINGFACToRoNHL60CELLANDSENSITIVITYoFCYTARABINEABSTRACTobjectivesT

2、oinvestigatetheeffectsofhumanrecombinantgranulocyte—clonystumimatingfactor(rhuG—CSF)onHL一60cellandeffectsonthesensitivityofcytarabineMethodsHL60cellwasculturedindifferentconcentrationofrhuGCSF(partone)，thechangeswereobse

3、rvedbycellmorphology，includingWright’S、acridineorange(AO)andethidiumbromide(EB)staining；MTTassay；nitrobluetetrazoliurn(NBT)；expressionofCDBThen(parttwo)HL一60cellwasculturedwithcytarabinewithorwithoutrhuG—CSF，morphology(W

4、ri曲t’SandAO／EBstaining)、MTTassaywereusedtoobservetheeffectsResultsHL60cellinthecontrolgrouphasnochangeAfterbeingculturedwithdifferentconcentrationsofrhuG—CSF48hours，themorphologychangescouldbeclearlyobserved：thecellunder

5、wentdeformation，decreasingvolume，bubblingThemembranesofthecellandthebubblewereintegralIfthecellwasstainedbyWright’S，thenuclearmembranesweresplitted，thepurplishredchromatinswereconcentratedanddividedintopieces，approachedt

6、onuclearmembranes，gatheredtotheedgeandformedstandardapoptosisbodiesDifferencescouldbeobservedwhentheODvalueoftheexperimentalgroupscomparingwiththecontrolgroupinMTTassay(p005)，somegroupsevenmadesignificantdifferences；(pO0

7、1)theODvalueofcontrolgroupwashigherthantheexperimentalgroups，andwiththeincreasingoftheconcentrationsofrhuG—CSF，theODvaluesdecreasedthereweredifferencesbetweentheexperimentalgroupstooWhenweobservedthecellapoptosisbyAO／EBs

8、taining，wegetaoppositetrend，withtheincreasingoftheconcentrmionsofrhuG—CSFtheapoptosispercentageincreasedComparedvvimcontrolgroupthereweresignificantdifferencesbytheexperimentalgroups(p001)thereweredifferencesevensignific

9、antdiffemcesbetweentheexperimentalgroupsWhilewhenwestudiedtheODvalueinNBTassayandtheexpressionpercentageofCDl3，wefoundnodifferencesInparttwo，thecellhadthesamechangesinmorphologyasinthepartoneThelowtohighsequenceofODvalue

10、inMTTassaywas：rhuG—CSFAra—C，Arac，rhuG—CSF，CON，thedifferencesamongthegroupsweresignificant(pO01)ThelowtohighsequenceofapoptosispercentageinAO／EBstainingwas：CON，rhuGCSF，Ara—C，AracrhuGCSF，thedifferencesamongthegroupsweresig