簡(jiǎn)介：內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文奶牛環(huán)境性乳腺炎滅活疫苗的研制及效果評(píng)價(jià)姓名趙建榮申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師郝永清20090501DEVELOPMENTANDEVALUATIONOFEFFECTIVENESSOFINACTIVATEDVACCINEOFBOVINEENVIRONMENTALMASTITISBSTRACT’MASTIFFSISINFLAMMATIONOFMAMMARYGLANDBECAUSEOFVARIOUSCAUSATIVEORGANISMMASTIFFSBECOMESMAINRESOILLGEOFDELETERIOUSBACTERIUMOFMILKANDMILKPRODUCTITALSOBECOMESODMMON謝也DISEASEOFMALLANDANIMALESPECIALLY，ETIOLOGICALFACTORSOFENVIRONMENTALMASTIFFSALEVERYCOMPLICATINGANDSPECIESOFCAUSATIVEORGANISMARERELATIVELYMUCHMORETHEREFORE，THEEXPERIMENTCALRYSOUTANALYSESOFIMMUNOGENICITYOFMAINCAUSATIVEORGANISMOFENVIRONMENTALMASTITISTHATHASBEENALREADYSEPARATEDANDIDENTIFIEDANDPREPARESVACCINEIHOPEEXPERIMENTWILLPROVIDENEWMETHODANDROUTETOPREVENTANDCBREENVIRONMENTALMASTITISININNERMONGOLIATOSELECTSTRAINSOFGOODIMMUNOGENICITYANDEXTENSIVEANTIGENSPECTRUM2SESCHERICHIACOLISAND2SKLEBSIELLASAREPROCESSED、現(xiàn)出SDSPAGEANDWESTERNBLOTTHERESULTSSHOWTHATTHEPROTEINBANDSOFALANDKLAREMOREFORTISTHANA2ANDK2TOPROGRESSTHOROUGHLYIMMUNOGENICITYANALYSIS，ELISAISESTABLISHEDTODETECTTHEANTIBODYTITERSANDTHECROSSPROTECTIVEANTIBODYINBLOODSERBINOFRABBITAFTERIMMUNIZATIONAMANDKLOFTYPICALANTIGENSPECTRUMANDALLROUNDPROTECTIVEANTIGENSALESUITABLETOBECOMESTRAINSOFPREPARINGVACCINETHEBACTERIALSUSPENSIONOFALANDKLISUNACTIVATED謝NLFORMALDEHYDESANDPROGRESSEDGERMFREETESTALHYDROGELADJUVANTISSELECTEDTOMAKEINACTIVATEDVACCINEAGAINSTENVIRONMENTALMASTITISANDTHEVACCINEISTESTED、析THSAFETYCOLLECTINGBLOODSERUMOFRABBITSAFTERTHREETIMESIMMUNIZATION、析也THEVACCINEISTESTEDSPECIFICANTIBODYBYMETHODOFELISATHERESULTSSHOWTHEVACCINEAGAINSTENVIRONMENTALMASTITISOFCOWSBYMIXING她也ALHYDROGELISSAFENEVACCINEALSOCANSUSCITATERABBITSTOPRODUCEHI曲SPECIFICANTIBODYWITHTHEMOUSEMODEL，CHALLENGETESTISPROCESSEDAFTERTHREETIMESIMMUNIZATIONWI吐LTHEVACCINETHERESULTSSHOWEFFECTIVEPROTECTIONAGAINSTESCHERICHIACOLIANDKLEBSIELLAOFHOMOLOGOUSANDVARIOUSSEROLOGICALTYPESCOULDBEGAINEDTOESTIMATEEFFECTOFPREVENTANDCU他BOVINEENVIRONMENTALMASTITISBYINOCULATINGINACTIVATEVACCINETOCARRYOUTEXPERIMENTOFINOCULATINGVACCINEFORCOWSINGRAZINGLANDOFINNERMONGOLIAAGRICULTURALUNIVERSITYEXPERIMENTESTABLISHESELISATOTESTSPECIFICANTIBODYINBLOOD翻胃瑚ANDNLI墩翻器瑚FOREVALUATINGEFFECTOFIMMUNITY，TESTINGSPECIFICANTIBODYINBLOOD∞NLMANDIILILKSERUMAFTERTHREETIMESIMMUNIZATION謝ⅡLTHEVACCINE，THERESULTSSHOWTHATSPECIFICANTIBODYOFIMMUNITYGROUPINBLOODSERUMANDIILI墩

簡(jiǎn)介：CLASSIFIEDINDEXCONFIDENTIALYES／NONOCODE10224NO120910799DISSERTATIONFORTHEMASTERDEGREERESEARCHONTHEEFFECTOFSYKONDAIRYCOWMAMMARYEPITHELIALCELLPROLIFERATIONCANDIDATELINLINSUPERVISORPROFESSORLINYEDEGREECATEGORYMASTEROFSCIENCECOLLEGECOLLEGEOFLIFESCIENCEFIRSTLEVELDISCIPLINEBIOLOGYSECONDLEVELDISCIPLINEBIOCHEMISTRYANDMOLECULARBIOLOGYHARBINCHINAJUNE2015東北農(nóng)業(yè)大學(xué)理學(xué)碩士學(xué)位論文245提取質(zhì)粒24246帶粘性末端質(zhì)粒的制備24247連接與轉(zhuǎn)化25248AKTL啟動(dòng)子的PGL3真核表達(dá)載體的鑒定。25249去內(nèi)毒素質(zhì)粒的提取252410去內(nèi)毒素質(zhì)粒轉(zhuǎn)染奶牛乳腺上皮細(xì)胞252411熒光素酶活性檢測(cè)2625數(shù)據(jù)處理與統(tǒng)計(jì)分析263結(jié)果與分析。2731奶牛乳腺組織中SYK的表達(dá)與定位27311奶牛乳腺組織中SYK的表達(dá)含量變化27312奶牛乳腺組織中SYK的表達(dá)部位2832SYK基因?qū)δ膛Ｈ橄偕掀ぜ?xì)胞增殖的影響29321奶牛乳腺上皮細(xì)胞原代培養(yǎng)29322奶牛乳腺上皮細(xì)胞的鑒定30323SYK基因沉寂對(duì)細(xì)胞增殖的影響31324SYK基因過(guò)表達(dá)對(duì)細(xì)胞增殖的影響3333SYK基因調(diào)節(jié)奶牛乳腺上皮細(xì)胞增殖的機(jī)制一37331AKTL基因啟動(dòng)子區(qū)PCR擴(kuò)增一37332PGL3AKTL真核表達(dá)載體構(gòu)建結(jié)果37333熒光素酶活性檢測(cè)結(jié)果374討侖3941奶牛乳腺組織中SYK基因的表達(dá)與定位分析3942SYK基因?qū)δ膛Ｈ橄偕掀ぜ?xì)胞增殖的影響一3943SYK基因調(diào)節(jié)乳腺上皮細(xì)胞增殖的機(jī)制405結(jié)論42致射43參考文獻(xiàn)44攻讀碩士期間發(fā)表的學(xué)術(shù)論文49II

簡(jiǎn)介：ISOLATIONANDCULTUREANDIDENTIFICATIONOFCOWMAMMARYSTEMCTHESISSUBMITTEDTOQINGDAOAGRICULTURALUNIVERSITYINFULFILLMENTOFTHEREQUIREMENTFORTHEDEGREEOFMASTEROFAGRONOMYLIUCHANGJUCOLLEGEOFANIMALSCIENCEANDVETERINARYMEDICANESUPERVISORPROFDONGYAJUANQINGDAOCHINAJUNE2009112試劑藥品17113試驗(yàn)儀器1712試驗(yàn)方法18121奶牛MASCS的分離及原代培養(yǎng)18122奶牛MASCS的傳代培養(yǎng)18123奶牛MASCS生長(zhǎng)曲線(xiàn)的繪制。19124奶牛MASCS的瑞氏吉姆薩RSGMS染色一19125奶牛MASCS的凍存與復(fù)蘇1913結(jié)果與分析20131奶牛MASCS的分離及原代培養(yǎng)～20132奶牛MASCS的傳代培養(yǎng)20133奶牛MASCS生長(zhǎng)曲線(xiàn)的繪制_21134奶牛MASCS瑞氏吉姆薩RSGMS染色2L135奶牛MASCS的凍存與復(fù)蘇2214討論222不同月齡奶牛MASCS的生物學(xué)特性比較2621試驗(yàn)材料和方法26211試驗(yàn)材料26212試劑藥品26213試驗(yàn)儀器26214試驗(yàn)分組26。’22試驗(yàn)方法27221不同月齡奶牛MASCS的分離及原代培養(yǎng)27222不同月齡奶牛MASCS的傳代培養(yǎng)27223不同月齡MASCS生長(zhǎng)曲線(xiàn)的繪制一27224不同月齡奶牛MASCS貼壁率的測(cè)定2723結(jié)果與分析28231不同月齡奶牛MASCS原代培養(yǎng)形態(tài)學(xué)觀察結(jié)果28232不同月齡奶牛MASCS傳代培養(yǎng)形態(tài)學(xué)觀察結(jié)果29

簡(jiǎn)介：STUDIESONGENETICVARIATIONOFHSFLGENEANDITSASSOCIATL0NⅥ礓THGLANDHEALTHINCATTLEBYⅪAPENGSUPERVISORPROFWANGGENLINATHESISSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFUWLLLMENTOFTHEREQUIREMENTSFORMASTERDEGREEOFAGRONOMYTHECOLLEGEOFANIMALSCIENCEANDTECHNOLOGYNANJINGAGRICULTURALUNIVERSITYNANJING，210095，PRCHINACOMPLETEDINAPRIL，2012COMMENCEMENTINMAY，2012㈣∞刪5Ⅲ刪1刪6刪3㈣2刪Y本研究由“十二五’’國(guó)家科技計(jì)劃課題編號(hào)2011BAD28802，2012BADL2810和江蘇省農(nóng)業(yè)三新工程課題編號(hào)SX2011189資助SUPPORTEDBYNATIONALSUPPORTINGPROJECTSFORSCIENCEANDTECHNIQUES州O2011BAD28802，2012BADL2810ANDPROVINCIALPROJECTFORAGRICULTURALTECHNIQUES科OSX2011189

簡(jiǎn)介：分類(lèi)號(hào)華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文大豆異黃酮與玉米赤霉烯酮對(duì)乳腺癌細(xì)胞系MCF一7聯(lián)合作用的的研究RESEARCHOFINTERACTIONOFIS0ANDZEAONBREASTCANCER研究生指導(dǎo)教師MCF7CELLS馬青山齊德生教授專(zhuān)業(yè)動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)研究方向飼料毒物與抗?fàn)I養(yǎng)因子獲得學(xué)位名稱(chēng)農(nóng)學(xué)碩士獲得學(xué)位時(shí)間華中農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院中國(guó)武漢華中農(nóng)業(yè)大學(xué)學(xué)位論文獨(dú)創(chuàng)性聲明及使用授權(quán)書(shū)學(xué)位論文是如需保密，解密時(shí)間二0／O年占月／P日是否保密獨(dú)創(chuàng)性聲明本人聲明所呈交的論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫(xiě)過(guò)的研究成果，也不包含為獲得華中農(nóng)業(yè)大學(xué)或其他教育機(jī)構(gòu)的學(xué)位或證書(shū)而使用過(guò)的材料，指導(dǎo)教師對(duì)此進(jìn)行了審定與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中做了明確的說(shuō)明，并表示了謝意。研究生簽名馬者以時(shí)間如。7年多月／日學(xué)位論文使用授權(quán)書(shū)學(xué)位敝儲(chǔ)張多荔厶翩張一丟岳譬一獅肌1幽／FEJ拼飆7㈣乃注請(qǐng)將本表直接裝訂在學(xué)位論文的扉頁(yè)和目錄之間

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文中藥乳頭灌注劑治療臨床型奶牛乳腺炎的研究姓名張振國(guó)申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師侯振中20090620ABSTRACTⅢSTUDIESONTRADITIONALCHINESEMEDICINEPERFUSIONSTOTREATCLINICALMASTITISINDAIRYCOWSABSTRACTCOWMASTITISHASGREATHARMTODAIRYCOWBREEDINGINDUSTRY．ITREDUCESMILKQUANTITYANDQUALITY,WHICHMAKETHEGREATECONOMICLOSSESTHEREFORE,ITISINCREASINGLYIMPORTANTHOWTOEFFECTIVELYPREVENTANDCURECOWMASTITISINTHISEXPERIMENT,THROUGHANTIBACTERIALTESTANDORTHOGONALTEST,SELECTEDSEVERALCHINESEHERBALMEDICINEWHICHWERESENSITIVETOCLINICALMASTITISPATHOGENICBACTERIAISOLATEDFROMCLINICALMASTITISCOWINHARBINTHEOPTIMALTRADITIONALCHINESEMEDICINECOMPOSITIONWASDETERMINEDBASEONTHERATIONALEOFTHETRADITIONALCHINESEMEDICINEPHARMACEUTICSCLINICALPHARMACODYNAMICSTESTANDSAFETESTOFTHENIPPLEPERFUSIONOFTHETRADITIONALCHINESEMEDICINEWASMADESIMULTANEOUSLY,WEDETECTEDTHECONTENTSOFSOD、MDA、NO、GSHPX、LDH、TAOCINWHEYBEFOREANDAFTERUSINGMEDICINEWHICHCOULDINDIRECTLYREFLECTTHESTATEOFIMMUNESYSTEMANDMAMMARYGLANDREPAIRTIONOFDAIRYCOWTHESTUDIESSHOWEDTHATMAINPATHOGENICBACTERIAOFCLINICALMASTITISWASSTREPTOCOCCUSMASTITIDIS,STAPHYLOCOCCUSAUREUSANDESCHERICHIACOLISEVENCHINESEMEDICINALHERBDANDELION,HEARTLEAFHOUTTUYNIAHERB,LIGUSTICUM,ANDSOONMADEUPCOMPOSITIONTHROUGHANTIBACTERIALTEST,ORTHOGONALTESTANDTHETHEORYOFVETERINARIANTHEPERFUSIONOFTHETRADITIONALCHINESEMEDICINEWASSAFETYTHROUGHSAFETESTTHEPERFUSIONHADGOODTHERAPEUTICACTIONONCLINICALMASTITISOFDAIRYCOWEFFECTIVERATE100,HEALINGRATE9091THECONTENTOFSOD,GSHPX,TAOCINWHEYAFTERUSINGDRUGWASOBVIOUSLYHIGHERTHANBEFOREP005,ANDTHECONTENTOFMDAANDNOINWHEYWASSIGNIFICANTLYLOWERTHANBEFOREP005,THECONTENTOFLDHDIDNOTCHANGESIGNIFICANTLYP＞005THESTUDIESSHOWEDTHATTHEPERFUSIONOFTHETRADITIONALCHINESEMEDICINESWASASAFEANDGOODEFFICACYDRUGTOCLINICALMASTITISWHICHCOULDPROVIDESOMEBENEFICIALATTEMPTFORPURFUSIONOFTHETRADITIONALCHINESEMEDICINETOTHEAPPLICATIONANDDEVELOPMENTOFSTOCKFARMINGKEYWORDSCOWS,MASTITIS,THETRADITIONALCHINESEMEDICINE,BREASTPERFUSIONS,ANTIOXIDANTSYSTEMCANDIDATEZHANGZHENGUOSPECIALITYCLINICALVETERINARYMEDICINESUPERVISORPROFHOUZHENZHONG

簡(jiǎn)介：977407HJ§掛L一FOJ西弛農(nóng)林釬捉大學(xué)2006屆攻讀碩士學(xué)位研究生學(xué)位畢業(yè)論文催乳素和TPA乳腺共表達(dá)載體構(gòu)建和細(xì)胞表達(dá)研究學(xué)科專(zhuān)業(yè)L哂匪置匡堂研究方向塾塑題照王猩研究生藍(lán)』生藍(lán)指導(dǎo)教帥堂插教授完成時(shí)間墊盟圭理且一中國(guó)陜目輛凌CONSTRUCTIONOFMAⅣ卟嗄ARYGLANDDUAL．EXPRESSIONVECTORCONTAININGPROLACTINANDTPAGENEANDITSEXPRESSIONINCELLSABSTRACTINTHISRESEARCH，PROLACTINGENEWASCLONEDFROMGUANGZHONGGOATANDTHEMAMMARYGLANDDUALEXPRESSIONVECTORPPTWASCONSTRUCTEDWHICHCONTAINEDPROLACTINANDTPAGENE．THENPPTWASTRANSFECTEDINTOMICEMAMMARYGLANDCANCERCELLSCULTURED／NVITROANDTHEEXPRESSIONCELLSTRAINSWEREABTAINED．HIGHLEVELEXPRESSIONVECTORWASPROVIDEDFURTHEMAMMARYGLANDBIOREACTORBYNUCLEARTRANSFER．THISRESEARCHCANHELPTOSOLVEPROBLEMONHOWTOINCREASETHEEXPRESSIONOFFOREIGNPROTEIN．THERESULTSOFRESEARCHISASFOLLOWING1．GOATPROLACTINEDNAWASCLONEDFROMGUANZHONGMILKGOATANTERIORPITUITARYRNAWITHRTPCR，WHICHWAS760BPANDCOMPRISEDINTACTCODINGREGIONS．SEQUENCEANALYSISDEMONSTRATEDTHATITSHOMOLOGYWITHTHERELATIVEREGIONOFPROLACTINONGENBANKWAS99％．ANDMUTATIONSCOULDNOTALTERPROTEIN’SSTRUCTUREANDFUNCTION．2．BYTHESTRATEGYOFDIRECTIONALCLONING，PROLACTINGENEWASINSERTEDTOMAMMARYGLANDEXPRESSIONVECTORPEBTANDMAMMARYGLANDDUALEXPRESSIONVECTORPPTWASCONSTRUCTEDWHICHCONTAINEDPROLACTINANDTPAGENE，WHICHINCLUDEDRESISTANTGENEANDEGFPREPORTGENE．ANDEGFPANDAIMEDGENEEXPRESSFUSIONPROTEIN．3．THEVECTORPPTWASTRANSFECTEDINTOMICEBOVINEEARFIBROBLASTMAMMARYGLANDCANCERCELLSCULTUREDINVITROUSINGLIPOSOME．AFTERG418FASTNESSSCREENING，THEPOSITIVECELLSLOWLEVELEXPRESSEDSTABLY．PCRIDENTIFICATIONDEMONSTRATEDTHATTHEVECTORHADINTEGRATEDINTOMICEMAMMARYGLANDCANERCELLULARCHROMOSOMES．THECELLSWHICHTRANSFECTEDPPTEXPRESSEDMOREEGFPPROTEINTHANCELLSTRANSFECTEDPEBTKEYWORDSPROLACTIN，MAMMARYGLANDDUALEXPRESSIONVECTOR，TRANSFECTION，EGFP

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文HMMEPDT致犬乳腺腫瘤細(xì)胞凋亡相關(guān)因子的研究姓名馬興乾申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師劉云20090620東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文IISTUDIESOFRELATEDAPOPTOSISFACTSONCANINEBREASTTUMCELLWITHHMMEPDTABSTRACTCANINEBREASTTUMOCCUREDFREQUENTLYINTHEVETERINARYCLINICSATPRESENTBREASTCANCERISALWAYSTREATEDBYSURGERYLONGTERMSURVIVALRATEHASN’TBEENIMPROVEDWITHTHEDEVELOPMENTOFTHEMODERNTECHNOLOGYPHOTODYNAMICTHERAPYPDTBECOMESANEWCHOICETOTREATMALIGNANTTUMBYPHOTOSENSITIZERLIGHTOXYGENMOLECULEPHOTOSENSITIZERCANBEABSBEDELECTIVELYBYPATHOLOGICALTARGETTISSUEACTIVEOXYGENWILLBEPRODUCEDIFITISSTIMULATEDBYLIGHTWITHSUITABLEWAVELENGTHTREATEDBYENERGYTRANSFMITINJURESTHETARGETTISSUESOITISATUMTHERAPYMETHODWITHGOODDEVELOPINGPROSPECTWHICHCANINDUCEAPOPTOSISNECROSISHEMATOPPHYRINMONOMETHYLETHERHMMEISANEWPHOTOSENSITIZERHASBEENPROVEDITSHIGHPERFMANCEINFOUNDATIONALCLINICALEXPERIMENTSBUTNOWRESEARCHESABOUTTHEEFFECTWITHHMMEPDTONCANINEBREASTTUMCELLARESTILLLESSATHOMEABROADTHENEWHMMETHEHENELASEROFWAVELENGHOF6328NMIRRADIATETHEBREASTTUMCELLLINEWASUSEDINTHISSTUDYTOINVESTIGATEMECHANISMOFPDTONCANINEBREASTCANCERCHMMCELLINDERTOPROVIDEABASISFTHETREATMENTOFCANINEBREASTCANCERDISEASECELLLINESWERECOLLECTEDISOLATEDFROMTHECLINICALMETASTATICBREASTCANCERCULTUREDNMALLYCELLSDURINGEXPONENTIALPHASEOFGROWTHWEREDIVIDEDINTOGROUPSBLANKCONTROLLASERGROUPPHOTOSENSITIZERGROUPCOMBINEDGROUPLASERILLUMINATIONPHOTOSENSITIZERHMMEPDTEXPERIMENTWASUSEDFCANINEBREASTCANCERCHMMCELLSTHEOD490WASDETECTEDWITHMTTALSOEFFECTOFPDTONTHECULTUREOFCANINEBREASTTUMCELLSINVITROWASDETECTEDINHIBITIONCURVEWASDRAWNTHENCELLSRATEWEREDETECTEDWITHTRYPANBLUEEXCLUSIONTESTAPOPTOSISWASDETECTEDWITHHOCHEST33258STAINNINGELECTROPHETICTECHNIQUECELLCYCLEWASDETECTEDWITHFLOWCYTOMETRYCONTENTOFMDANOACTIVITYOFSODGSHPXWEREDETECTEDWITHCHROMATOMETRYCONTENTOFIL2IL6WASDETECTEDWITHRADIOMMUNOASSAYTHEWEREASFOLLOWSBLANKCONTROLPHOTOSENSITIZERGROUPHMME20GMLLASERGROUP28JCM2HADNOOBVIOUSEFFECTONTHECELLMULTIPLICATIONBUTTHEHMMEPDTHMME20GML28JCM2GROUPCELLMULTIPLICATIONWASOBVIOUSLYINHIBITEDSOITPROVEDCANINEBREASTCANCERCHMMCELLSINVITROWEREINHIBITEDWITHHMMEPDTWITHINCERTAINPREMISESPHOTODYNAMICEFFECTPHOTOSENSITIZERDENSITYILLUMINATIONDOSESHADRELATIONSAFTERHMMEPDTTHEREWASOBVIOUSCELLULARAPOPTOSISAPOPTOSISWASMEOBVIOUSASTIMEPROLONGINGATDIFFERENTTIMEINCELLCYCLERATEINCREASEOFG0G1PHRASEHADSIGNIFICANTDIFFERENCEAT12H24H48HCOMPAREDWITHTHECONTROLGROUPP001HOWEVERRATEDECREASEOFSPHRASE

簡(jiǎn)介：分類(lèi)S8167UDC636學(xué)校代碼10129學(xué)號(hào)2009201031瘦素對(duì)奶牛乳腺上皮細(xì)胞乳蛋白合成的調(diào)控作用研究EFFECTSOFLEPTINONMILKPROTEINSYNTHESESINBOVINEMAMMARYEPITHELIALCELLS申請(qǐng)人王春艷學(xué)科門(mén)類(lèi)農(nóng)學(xué)學(xué)科專(zhuān)業(yè)動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)研究方向反芻動(dòng)物營(yíng)養(yǎng)調(diào)控理論與技術(shù)指導(dǎo)教師高民研究員論文提交日期二〇一二年五月EFFECTSOFLEPTINONMILKPROTEINSYNTHESESINBOVINEMAMMARYEPITHELIALCELLSABSTRACTINTHISEXPERIMENT,EPITHELIALCELLSCULTURETECHNIQUESANDCELLBIOLOGYANDMOLECULARBIOLOGYTECHNIQUESWEREUSEDTOSTUDYLEPTINONMILKPROTEINSYNTHESESOFBOVINEMAMMARYEPITHELIALCELLSTHEFINDINGSINCLUDETHREEPARTSASFOLL0W1MAMMARYEPITHELIALCELLSWEREOBTAINEDFROMTHELACTATINGHOLSTEINCOWSBYENZYMEDIGESTIONANDCULTUREDSUCCESSFULLYINVITROTHECELLSAFTERPURIFIEDBYTRYPSINWERESTOREDINLIQUIDNITROGEN,ANDDETECTEDTHESURVIVALRATEOFRECOVEREDCELLSIMMUNOFLUORESCENCESTAININGSKELETONPROTEINKERATININDICATEDTHATTHECELLSWECULTUREDREALLYWEREBOVINEMAMMARYEPITHELIALCELLSOBSERVINGFROMINVERTEDMICROSCOPE,CELLSWERECOBBLESTONEAPPEARANCEANDADHERENCETHERESULTSSHOWEDTHATTHECELLSWEHARVESTEDCANBEUSEDTOTHEFOLLOWINGSTUDIES2ONTHEBASICOFCULTURESYSTEMOFMAMMARYEPITHELIALCELLSINVITRO,THETREATMENTSOFCELLSINCLUDED4DOESOFLEPTIN0,10,100AND1000NG/MLWITH1ΜG/MLORWITHOUT0PROLACTINPRL,AND0NG/MLLEPTINWASTHECONTROLGROUPTHERESULTSOFDETECTINGCELLPROLIFERATIONBYMTTWERETHATLEPTINPROMOTEDCELLPROLIFERATIONASTIMEDEPENDENCEANDATTHESAMETIME,10AND100NG/MLLEPTINUNCOMMONLYPROMOTEDCELLPROLIFERATION,BUT1000NG/MLLEPTINSIGNIFICANTLYINHIBITEDPROLIFERATIONTHEEFFECTWITHOUTPRLISNOTNOTABLE3THEEXPERIMENTDESIGNOFTHISPARTWASSAMETOPART2THEEXPRESSIONOFGENESWASFROMRTPCREXPERIMENTSANDPROTEINSECRETIONWASANALYZEDBYELISAKITTHERESULTSSHOWEDTHAT1THEEXPRESSIONOFMAINMILKPROTEINGENESWASENHANCEDBYLEPTININTHEPRESENCEOFPRL,ANDTHEREWEREDIFFERENTEFFECTSWITHDIFFERENTLEPTINTREATMENTS21000NG/MLLEPTININHIBITEDPRLRECEPTORPRLREXPRESSIONSIGNIFICANTLYWITHPRLANDPRLOBVIOUSLYINDUCEDPRLREXPRESSION3THEEXPRESSIONOFJAK2WASSAMETOTHATOFMILKPROTEIN,BUTTHEREWASNOEFFECTOFLEPTINONTHEEXPRESSIONOFSTAT54NOEFFECTOFLEPTINONTHESECRETIONOFΑCASIENPROTEIN,10NG/MLLEPTINHASTENEDTHESECRETIONOFΒCASIENPROTEINAND1000NG/MLLEPTININHIBITEDTHATSIGNIFICANTLYWITHPRLTHESTUDYISAUSEFULEXPLORATIONONMOLECULEINTERCOMBETWEENMAMMARYFATPADANDEPITHELIALCELLSANDINORDERTOLAYATHEORETICALFOUNDATIONFORFUTUREPRACTICALAPPLICATIONOFANIMALHUSBANDRY

簡(jiǎn)介：西北農(nóng)林科技大學(xué)碩士學(xué)位論文豬瘟病毒E2基因乳腺特異表達(dá)載體的構(gòu)建與細(xì)胞表達(dá)的研究姓名顧玲申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師張涌20040601豬瘟病毒E基因乳腺特異表達(dá)載體的構(gòu)建與細(xì)胞表達(dá)的研究ABSTRACTCLASSICALSWINEFEVERISAHARMFULDISEASETOSWINEHUSBANDRYCLASSICALSWINEFEVERVIRUSCSFVISITSCAUSEANDITSMAINPROTECTIVEANTIGENISGLYCOPROTEINE2ANIMALSCANPREVENTFROMDEADLYVIRUSSATTACKIFTHEYVACCINATEPROTEINE2COMINGFROMEUKARYOTICEXPRESSIONSYSTEMAHEADLYPRODUCINGBIOACTIVEPROTEINSTHROUGHMAMMARYGLANDBIOREACTORISAHOTFOCUSINTHEFIELDOFBIOLOGYANDTHEKEYQUESTIONISTHECONSTRUCTIONOFEXPRESSIONVECTORINMAMMARYGLANDBETALACTOGLOBULINBLGISTHEMAINPROTEMMONALRUMINANTMILKFORTHEPURPOSEOFCONSTRUCTINGMAMMARYGLANDSPECIFICEXPRESSIVECTORWEUSETHEUPDREAMREGULATIONSEQUENCEOFBOVINEBLGTOREGULATECSFVE2GENETOEXPRESSINMAMMARYEPITHELIALCELLSOVERIFYTHEEXPRESSIONEFICIENCYOFFOREIGNGENEANDRATIONALITYOFRPOSITIVECLONECELLSARESELECTEDANDPURIFIEDBYTHEEXPRESSIONANANEOANDREPORTGENEEGFPSOTHECELLSCANBEUSEDASSOURCGENEASPROMOTERINVITROINORDERTEXPRESSIONALVECTOANTIBIOTICSGENEKTOCREATETRANSGENIOFESCANIMALSBYNUCLEARTRANSFERTECHNIQUESTHERESULTSOFRESEARCHAREASFOLLOWINGBOVINEBLGGENEUPDREAMREGULATIONSEQUENCEISCLONEDBYMETHODOFPCRFROMCOWBLOODITCONSISTSOF5FLANKSEQUENCEABOUT645BPANDTHESIGNALPEPTIDESEQUENCEINFIRSTEXONABOUT92BPAFTERPCRPRODUCTISRECOVEREDANDPURIFIEDITISCLONEDINTOTSITEOFPNM18TVECTORTHENITISSEQUENCEDANDCOMPAREDWITHOTHERBOVINEBLGGENEINGENBANKTHERESULTSINDICATETHEHOMOLOGYAREALLABOVE9000ATLASTTHESEQUENCEISANALYZEDBYCOMPUTERWEFINDTHATMANYFACTORSORPROTEINBINDINGSITESANDRESPONSEELEMENTSUCHASMAMMARYSPECIFICBINDINGFACTORMSBFNUCLEARFACTOR1NF1RETINOICACIDRESPONSEELEMENTRAREANDTPARESPONSEELEMENTTREAREINCLUDEINITITSUGGESTESTHECLONEDREGULATORYELEMENTCOULDDIRECTFOREIGNGENESTOEXPRESSSPECIFICALLYINMAMMARYGLANDEPITHELIACELLSANDITCOULDBEUSEDTOCONSTRUCTMAMMARYGLANDSPECIFICEXPRESSIONALVECTORBLGREGULATIONSEQUENCEANDCSFVE2GENEARELINKEDBYMETHODOFRECOMBINANTPCRANDTHENTHEYAREINSERTEDINTOPMD18TVECTORTHEPOSITIVERECOMBINANTPLASMIDISMARKEDASPBETHECOMPOSEDFRAGMENTBEISOBTAINEDBYDIGESTINGPBEWITHRESTRICTIVEENZYMEHINDIIXBAIANDASEIVECTORPEGFPCIISALSODIGESTEDBYASEIANDISLINKEDWITHBEFRAGMENTBYT4DNALIGASEAFTERITS5ENDISDEPHOSPHATASEDSOTHEMAMMARYGLANDSPECIFICEXPRESSIONALVECTORISCONSTRUCTEDWHICHCONTAINSREPORTGENEEGFPANTIBIOTICSGENEKANANEOREGULATORYELEMENTBOVINEBLGGENEUPSTREAMREGULATIONFRAGMENTANDFOREIGNTARGETGENECSFVE2GENE

簡(jiǎn)介：EXPRESSIONSOFCLAUDIN1、ECADHERINANDTNF僅INCANINE艦～MMARYTUMOURSBYWANGFANGSUPERVISORPROFZHANGHAIBINATHESISSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFULFFILMENTOFTHEREQUIREMENTSF0RTHEMASTERDEGREEOFAGRONOMYCOMPLETEDINOCT2009COMMENCEMENTINDEC2009原創(chuàng)性聲明本人鄭重聲明所呈交的學(xué)位論文，是本人在導(dǎo)師的指導(dǎo)下，獨(dú)立進(jìn)行研究工作所取得的成果。除文中已經(jīng)注明引用的內(nèi)容外，本論文不包含任何其它個(gè)人或集體已經(jīng)發(fā)表或撰寫(xiě)過(guò)的作品成果。對(duì)本文的研究做出重要貢獻(xiàn)的個(gè)人和集體，均已在文中以明確方式標(biāo)明。本人完全意識(shí)到本聲明的法律結(jié)果由本人承擔(dān)。學(xué)位論文作者需親筆簽名盈％W唧年F胡，F(xiàn)日學(xué)位論文版權(quán)使用授權(quán)書(shū)本學(xué)位論文作者完全了解學(xué)校有關(guān)保留、使用學(xué)位論文的規(guī)定，同意學(xué)校保留并向國(guó)家有關(guān)部門(mén)或機(jī)構(gòu)送交論文的復(fù)印件和電子版，允許論文被查閱和借閱。本人授權(quán)南京農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存和匯編本學(xué)位論文。，保密口，在土年解密后適用本授權(quán)書(shū)。本學(xué)位論文屬于不保密講請(qǐng)?jiān)谝陨戏娇騼?nèi)打“寸’學(xué)位論文作者需親筆簽名每％≯∞年I明，，日導(dǎo)師需親筆簽名矽1年F厶月，，日

簡(jiǎn)介：EFFECTSOFGLUTAMINEONPROLIFERATIONANDHSP70EXPRESSIONOFMAMMARYEPITHELIALCELLSINDAIRYCOWSBYSHIHONGXIASUPERVISORPROFESSORWANGGENLINATHESISSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFULFILLMENTOFTHEREQUIREMENTSFORMASTERDEGREENANJINGAGRICULTURALUNIVERSITYNANJING，210095，PRCHINACOMPLETEDINNOVEMBER2009COMMENCEMENTINDECEMBER2009原創(chuàng)性聲明本人鄭重聲明所呈交的學(xué)位論文。是本人在導(dǎo)師的指導(dǎo)下，獨(dú)立進(jìn)行研究工作所取得的成果。除文中已經(jīng)注明引用的內(nèi)容外，本論文不包含任何其他個(gè)人或集體已經(jīng)發(fā)表或撰寫(xiě)過(guò)的作品成果。對(duì)本文的研究做出重要貢獻(xiàn)的個(gè)人和集體，均已在文中以明確方式標(biāo)明。本人完全意識(shí)到本聲明的法律結(jié)果由本人承擔(dān)。學(xué)位論文作者需親筆簽名生妞役，加聽(tīng)年殷月／／日學(xué)位論文版權(quán)使用授權(quán)書(shū)本學(xué)位論文作者完全了解學(xué)校有關(guān)保留、使用學(xué)位論文的規(guī)定，同意學(xué)校保留并向國(guó)家有關(guān)部門(mén)或機(jī)構(gòu)送交論文的復(fù)印件和電子版，允許論文被查閱和借閱。本人授權(quán)南京農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存和匯編本學(xué)位論文。保密口，在一年解密后適用本授權(quán)書(shū)。本學(xué)位論文屬于不保密口。請(qǐng)?jiān)谝陨戏娇騼?nèi)打“√’’學(xué)位論文作者需親筆簽名生乏L／嫠剔蒜勤獬陟卜劬侔皮月I多日勱叫年投其“B

簡(jiǎn)介：大連理工大學(xué)博士學(xué)位論文抗奶牛乳腺炎特異性卵黃抗體的制備及活性研究姓名甄宇紅申請(qǐng)學(xué)位級(jí)別博士專(zhuān)業(yè)生物化工指導(dǎo)教師徐永平20081101抗奶牛乳腺炎特異性卵黃抗體的制備及活性研究凝塊得到明顯改善；體細(xì)胞數(shù)和細(xì)菌計(jì)數(shù)顯著降低。IGY和青霉素對(duì)實(shí)驗(yàn)性乳腺炎的治愈率分別為83．3％和66．7％，對(duì)臨床型乳腺炎的治愈率分別為50％和33．3％。綜上所述，本研究成功制備出抗奶牛乳腺炎致病菌特異性IGY，考察了其體內(nèi)外活性，并證明了其對(duì)奶牛乳腺炎的療效優(yōu)于青霉素。為特異性IGY替代抗生素治療奶牛乳腺炎提供了依據(jù)。關(guān)鍵詞奶牛乳腺炎；卵黃免疫球蛋白；金黃色葡萄球菌；大腸桿菌

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文紫杉醇致體外培養(yǎng)犬乳腺腫瘤細(xì)胞凋亡形態(tài)學(xué)及抗氧化功能影響的研究姓名任曉麗申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師劉云20090620ABSTRACTISTUDIESOFPACLITAXELONMORPHOLOGYOFAPOPTOSISANDEFFECTOFANTIOXIDEFUNCTIONOFCANINEBREASTTUMORCELLINVITROCULTUREABSTRACTAPOPTOSISWHICHOCCURREDINALLTHETUMORTISSUESWASCLOSELYRELATEDTOTHEOCCURRENCE,DEVELOPMENTANDMETASTASISOFTUMORCHEMOTHERAPEUTICDRUGACHIEVEDTHETHERAPEUTICEFFECTMAINLYBYINDUCINGAPOPTOSISOFCANCERCELLSTHEREFORE,INDUCINGAPOPTOSISOFTUMORCELLSBECAMEAHOTSPOTFORTUMORTREATMENTRESEARCHNATURALMEDICINESWHICHHADEXTENSIVESELECTIVITY,LITTLETOXICSIDEEFFECTANDLOWDRUGRESISTANCEWEREIMPORTANTTENDENCYOFSTUDYINGONANTITUMORWITHCHINESEMEDICINESPACLITAXELWASANEWNATURALBROADSPECTRUMANTITUMORDRUG,BYWHICHTHEMECHANISMOFAPOPTOSISINDUCEDWASTOLEADTOCELLCYCLEARRESTING2/MPHASEMAINLYBYRESTRAININGMITOSISOFCELLS,THUSTHENORMALMITOSISOFCELLSANDCELLMULTIPLICATIONWEREINTERRUPTEDCANINEBREASTTUMORWASAKINDOFTUMORDISEASEWHICHWASCLINICALLYCOMMONFORVETERINARYTHESTUDYOFEFFECTONCANINEBREASTTUMORWITHPACLITAXELHASN’TBEENREPORTEDYETHOMEANDABROAD,ANDDOGHADACLOSERELATIONWITHHUMAN,ALSOTHEREWASSIMILARASPECTINENVIRONMENTANDCAUSEOFCANCERBETWEENDOGANDHUMAN,SODOGSWEREUSEDASTUMORMODELANDSTUDIED,THEMECHANISMOFANTICANCEROFANIMALTUMORCELLSINDUCEDBYPACLITAXELWASAPPROACHED,WHICHPLAYEDAVERYIMPORTANTROLEINTREATINGANIMALTUMORANDTREATMENTSCHEMEFORHUMANCANCERTHISSUBJECTTOOKTHECANINEBREASTTUMORCELLSLINESCHMMSTRAINCULTUREDINVITROASAMODELTHECHMMWASTREATEDWITHDIFFERENTCONCENTRATIONSOFPACLITAXEL,CELLMULTIPLICATION,CYTOTOXICEFFECT,CHANGEOFCELLMORPHOLOGYANDANTIOXYGENFUNCTIONFACTORSUCHASSOD,CAT,MDAWEREDETECTEDAT24H,48HAND72HAFTERCULTURED,THETREATMENTTIMECONCENTRATIONEFFECTRELATIONSHIPWASANALYZEDANDTHECHANGEOFCELLMORPHOLOGYANDOXYFREERADICALSOFAPOPTOSISINDUCEDBYPACLITAXELWEREAPPROACHED,WHICHCOULDPROVIDEMORERELIABLEEXPERIMENTALDATAFORFURTHERSTUDYONTHEMECHANISMOFAPOPTOSISOFCANINEBREASTTUMORCELLSINDUCEDBYPACLITAXELASWELLASTHECLINICALAPPLICATIONOFPACLITAXELFORTHETREATMENTOFCANCERTHEEXPERIMENTALRESULTINDICATEDTHAT1CHANGEOFCANINEBREASTTUMORCELLSGROWTHEFFECTEDBYPACILITAXELWITHMTTCHROMATOMETRYANDTRYPANBLUEEXCLUSIONEXPERIMENTSWASDETECTED,THERESULTSINDICATEDINHIBITIONRADIOGRADUALLYINCREASEDWITHINCREASEOFPACLITAXELCONCENTRATIONANDCULTURETIME,BUTCELLSACTIVITYDECREASEDGRADUALLY,WHICHWASINTIMEDOSEDEPENDENTMANNERBYDETECTINGTHELACTICACIDDEHYDROGENASELDHACTIVITY,THERESULTSHOWEDTHATTHEREWERESIGNIFICANTDIFFERENCEBETWEENPACLITAXELGROUPSWITHDIFFERENTCONCENTRATIONSATTHESAMETIME（P005）,OTHERGROUPSHADSIGNIFICANT

簡(jiǎn)介：關(guān)于學(xué)位論文原創(chuàng)性和使用授權(quán)的聲明本人所呈交的學(xué)位論文，是在導(dǎo)師指導(dǎo)下，獨(dú)立進(jìn)行科學(xué)研究所取得的成果。對(duì)在論文研究期間給予指導(dǎo)、幫助和做出重要貢獻(xiàn)的個(gè)人或集體，均在文中明確說(shuō)明。本聲明的法律責(zé)任由本人承擔(dān)。本人完全了解山東農(nóng)業(yè)大學(xué)有關(guān)保留和使用學(xué)位論文的規(guī)定，同意學(xué)校保留和按要求向國(guó)家有關(guān)部門(mén)或機(jī)構(gòu)送交論文紙質(zhì)本和電子版，允許論文被查閱和借閱。本人授權(quán)山東農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索，可以采用影印、縮印或其他復(fù)制手段保存論文和匯編本學(xué)位論文，同時(shí)授權(quán)中國(guó)科學(xué)技術(shù)信息研究所將本學(xué)位論文收錄到中國(guó)學(xué)位論文全文數(shù)據(jù)庫(kù)，并向社會(huì)公眾提供信息服務(wù)。保密論文在解密后應(yīng)遵守此規(guī)定。論文作者簽名瑚墜英文縮略詞乳腺炎‘葡萄球菌屬金黃色葡萄球菌鏈球菌屬停乳鏈球菌乳房鏈球菌大腸桿菌最小抑菌濃度加利福尼亞隱性乳腺炎檢測(cè)法JIIIIIRILITIILI,FLLLLLLIJ肌Y1786791。MASTITISGENUSSTRAPHYLOCOCCUSSTRAPHYLOCOCCUSAUREUSGENUSSTREPTOCOCCUSSTRAPHYLOCOCCUSDYSGALACTIAESTRAPHYLOCOCCTISUBEFISESCHEFI【CHIACOLIMICCMTCALIFOMIAMASTIFFSTEST