海島棉NBS-LRR抗性基因類似序列的分離及Gbarvin基因的鑒定.pdf

1、中國農業(yè)科學院碩士學位論文海島棉NBSLRR抗性基因類似序列的分離及Gbarvin基因的鑒定姓名：盛剛申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：李付廣王濤20050601Abstractverticilliumwilthasbecomeoneofthel@ostseverediseasesofoolloninChiuaforyearsMorover也eresistancetoverlicilliumwiItisdi任bult

2、tobepopularizedjncultivatedcoltonspeciesGossypiumhirsutumLbycrossbreedingfromGossypiumbarbadenseL，whichhashighresistancetoSofarmorethan50genesresistanttodifferentpathogens，includingbacteria，fungi，nematodesandviruses，have

3、beenclonedinplantAlloftheseplantdiseaseresistancefR)geneshavetheconservedNBSandLRRdomain，althoughtheiroverailsequencehomologyislowInthisthesis，theNBSLRRgenesandresistancegeneanalogs(RGAs)areisolatedfromthegenomeofGossypi

4、umbarbadenseLXH21bypolymerasechainreaction(PCR)basedapproachwithdegenerateoligonucleotidepfimersdesignedfromtheseconserveddomainsThecharacteristicorganizationandevolutionofthesenoveldiseaseresistancegenesinGossypiumbarba

5、denseLgenomesareidentified，whicharesignificantforunderstandingofplantpathogeninteractionsanddevelopmentofnovelapproachestoeffectivecontrolofplantpathogensinagricultureThestudyoftheGossypiumbarbadenseLXH2lmaingenesexpress

6、ioninducedbyVerticilliumdahliaeKleb，issignificantnotonlyforunderstandingtheinteractionmechanismbetweenfungiwithhostbutalsoforapplyinggenestomolecularbreedinginGossypiumhirsutumLServelpathogenesisrelated(PR)proteinswillbe

7、producedinplantpathogeninteractions，includingPR4proteinsOneclassofPR_4(classer)containstwodomains，oneischitinbindingdomain，andanotheriscatalyticdomainwhichhavemanybiologicaifunctionandtheantifongaiactivityisanimportanton

8、eofthemClasslproteinscontainallNterminalcysteine。richehitinbindingdomainaGlyorProrichlinkcrregionandaCterminalcatalyticdoraairLBarwinlikeproteinisolatedfrombarlybelongstothisclass，havingriboucleaseactivitybesidesantifung

9、aiactivityTodate，、，eland、，e2isolatedfromtomato，aferesistanttoVerticilliumdahliaeandbothofthemcontaintheconservedLRRdomainGbarvin，whichencodes2ll鋤inoacids，hasachitinbi棚ingdomaininNterrainaiandabarwindomaininCterminaiInthi

10、sresearchitwasisolaredfromthecDNAofcultivarXH21(GossypiumbarbadenseL)，whichwasinducedbyVerticilliumdahlia，byapolymerasechainreaction(PCR)basedapproachwithdegenerateoligonucleotideprimersdesignedfromtheconservcdmotifsnVel

11、andVe2Itwasexpressedin點cob餌L21)andwasshowedresistanttoVerticil]iumdahliaeItwasproposedthatGbarvinhasriboucleaseactivityduetoitsnucleotidebindingsiteThesecondstructureofGbarvinissimilartopartofthoseofVelandVe2Itindicatest

12、hatGbarvinadoptsthesamemechanismforantifungalacitivityasVelandVe2，TheresiduesinvolvedinnucleotidebindingformanactivesiteaccordingtoitstertiarystructureRNAinterference(RNAi)resultsoftheGbarvininArabidopsisthaliana(columbi