雞J亞群白血病病毒與網(wǎng)狀內(nèi)皮增殖病病毒混合感染發(fā)病機理的研究.pdf

1、中國農(nóng)業(yè)大學博士學位論文雞J亞群白血病病毒與網(wǎng)狀內(nèi)皮增殖病病毒混合感染發(fā)病機理的研究姓名：劉思當申請學位級別：博士專業(yè)：基礎(chǔ)獸醫(yī)學指導教師：趙德明20050501AbstractPathologicalmodelwasestablishedincommercialbroilersatonedayoldthatinoculatedbyavianleukosisvirussubgroupJ(ALVJ)strainNX0101，reticul

2、oendotheliosisvirus(REV)strainSD9901andALVJREVtocontrastpathogenesisindifferentthebroilergroupsPathologyImmunosuppressjonandREVviraldeterminationincoinfectedbroilerswasmajorstuding，Primarystudyshowedthatbodyweightofbroil

3、ersinfectedwithALVJorREVcomparedwiththecontrol，wassignificantlydepressedandantibodyresponsetoNDVvaccinewasinhibitedInaddition，thesebroilersweresusceptibletobacterialagents，suchascolihacillosis，causinghigherlethalityratei

4、nearlytimeAllthatabovewasevenmoteseriousinbroilersinfectedwithALVJREVApoptosisoflymphocytesinbonemarrowspleen，thymusandbursawasdetectedbylightmicroscope，electronmicroscopeandterminaldeoxynucleotidyltransferaseUIPnickendl

5、abeling(TUNEL)TheresultsshowedALVJcouldinduceincoordinateapoptosisoflymphocytesinthymus，bursaandspleen，whichwasmainlyfoundinearlytime(intwoweeks)AndtheapoptosiswasmuchmoreobviousinthymusthaninotherimmuneorgansSoitwasconc

6、ludedthattheapoptosisoflymphocyteswasthemainreasonthatledtoatrophyofimmuneorgansandimmunosuppressionofbodyDynamicchangesofhistopatbologywerestudiedonimmuneorgansandsomevisceralorganssuchasliverkidneycardiacmuscleandlungT

7、heresultsshowedthatREVchieflycausedhyperplasiaofreticulocytesandlymphocytesinbonemarrowwhileALVJmainlyconducedhyperplasiaofeosinocytesThelesionsofbonemalTowinchickensinfectedwithALVJREVweremoreseriousthanthatjnfectedwith

8、REValonebutdifferenttothatinfectedwithALvJaloneThesedatashowedthatbonemarrowwasveryimportantindiseasescausedbyALVJorREVTherefore，lesionsofbonemarrowweretheessentialreasonmakingimmunosuppressionThematerialatrophyinotherim

9、muneorgans，whichwasrelatedtolesionsofbonemalTOWandapoptosisornecrosisoflymphocytas，beingseriouswhenchickensinfectedwithALVJREVthenfollowedbychickensinfectedwithREValone，wasalsotheimpoaantreasonfbrimmunosuppressionInother

10、svisceralorgans，hyperplasiaoftumorouseosinocytesinchickensinfectedwithALVJandhyperplasiaoftumorousreticulocyteswithlymphocytesinchickensinfectedwithREVwerefoundaroundthebloodvesselFromthe1esionsoforgans，itcouldbeseenthat

11、theimmunosuppresioninchikensinfectedwithALVJREVwasmuchmoreseriousthanthatcausedbyALVJorREValone，howeveaitwasnotfoundthatREVcanacceleratetheariseoftumorscausedbyALVJinearlytimeTostudythedistributionofREVintissusesandorgan