重組長效山羊FSH基因在中國倉鼠卵巢細胞表達的研究.pdf

1、揚州大學(xué)碩士學(xué)位論文重組長效山羊FSH基因在中國倉鼠卵巢細胞表達的研究姓名：嚴正杰申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：丁家桐20050501罡至鑾!重望簍墼生差!塑墨墾壟!堡魚墾塑墨塑墮耋壟箜堡窒一3Studiesofexpressionofrecombinantgoatlong。actingFSHagonistinChinesehamsterovarycellsMSCandidate：YanZhengjieSuperv

2、isor：ProfDingJiatong(CollegeofAnimalScienceandTechnology，YangZhouUniversity，China)AbstractFollicle—stimulatinghormone(FSH)isapituitaryglycoproteinhormoneItisessentialforthedevelopmentofovarianfolliclesandtesticularsemini

3、feroustubulesFSHisoftenusedinembryotransfer(ETlandsuperovulatoryBecausehalflifeofFSHisveryshortincirculationSOitneedrepeatedinjectionintherapyToaddressthispoint，thisstudyconstructchimericgenescontainingthesequenceencodin

4、gtheC—terminalpeptide(CTP)fusedtothetranslatedsequenceofthegoatFSH3subunitCTPisuniqueCOOH_terminalregionofthepsubunitofhumanchoriogonadotropinIthas28一residuepeptidesfrompositions118through145TherearefourcarbohydratesviaO

5、—linkagetoserineresiduesinthispeptidebackboneSialicacidofcarbohydratescanprolongthehalflifeofFSHincirculationDesigningspecificprimerscontainingKozaksequence，F(xiàn)SHpandhCG13astemplates，ampiifythechimericgeneFSH13CTPthroughtw

6、oPCRs，DigestandligateFSH瑾，F(xiàn)SH0一CTPwithdualespressionvectorpVOTROusingrestrictionendonucleasesandDNAligaseConsequently，F(xiàn)SHc【andFSH13geneswereinsertedintoplasmidpVITROGenesequencingindicatedtheexpressionvectorpVITROFSH0【B—