腫瘤細胞多亞細胞室遞送阿霉素酸敏膠束的制備及其抗腫瘤活性研究.pdf

1、腫瘤細胞多亞細胞室遞送阿霉素酸敏膠束的制備及其抗腫瘤活性研究腫瘤細胞多亞細胞室遞送阿霉素酸敏膠束的制備及其抗腫瘤活性研究崔晗培養(yǎng)類別全日制學位類型學術學位一級學科(專業(yè)類)藥學二級學科(專業(yè))藥劑學研究方向腫瘤靶向遞藥指導教師周四元教授培養(yǎng)單位藥學系藥劑學教研室二O一五年五月分類號R94UDC615.1密級公開第四軍醫(yī)大學碩士學位論文目目錄錄縮略語表............................................

2、......................................................................................................1中文摘要.................................................................................................................

3、.................................2Abstract.....................................................................................................................................................5前言..........................

4、................................................................................................................................8文獻回顧.......................................................................................

5、...........................................................9正文........................................................................................................................................................25第一部分

6、棕櫚酸聚乙二醇葉酸膠束的制備及其抗腫瘤活性研究..........................................251材料....................................................................................................................................251.1儀器...........

7、..............................................................................................................251.2試劑.......................................................................................................

8、..................261.3細胞及動物..............................................................................................................262方法.............................................................................

9、.......................................................262.1TPPDOX的合成.....................................................................................................262.2棕櫚酸聚乙二醇葉酸（PAhydPEGFA）的合成.....................

10、........................272.3DOXTPPDOX@PAhydPEGFA的制備............................................................282.4膠束的特性表征................................................................................................

11、......282.4.1DOXTPPDOX@PAhydPEGFA的粒徑及形態(tài).....................................282.4.2載藥量與包封率的測定...............................................................................292.4.3DOXTPPDOX@PAhydPEGFA表面元素分析...............

12、......................292.4.4臨界膠束濃度（CMC）的測定..................................................................292.5DOXTPPDOX@PAhydPEGFA穩(wěn)定性考察....................................................292.6DOXTPPDOX@PAhydPEGFA體外釋藥

13、........................................................302.7DOXTPPDOX@PAhydPEGFA的體外細胞毒性............................................302.8外源性葉酸對DOXTPPDOX@PAhydPEGFA細胞毒性的影響....................302.9DOXTPPDOX@PAhydPEGFA對腫瘤細胞

14、凋亡的誘導作用.........................302.10腫瘤細胞對DOXTPPDOX@PAhydPEGFA的攝取....................................312.11DOXTPPDOX@PAhydPEGFA轉運DOX在腫瘤細胞中的分布................312.12DOXTPPDOX@PAhydPEGFA的體內抗腫瘤活性............................

15、..........323結果....................................................................................................................................323.1TPPDOX的結構鑒定.........................................................

16、....................................323.2PAhydPEGFA的結構鑒定...................................................................................343.3膠束的表征.......................................................................

17、.......................................363.4載藥膠束體外釋藥曲線..........................................................................................393.5DOXTPPDOX@PAhydPEGFA的體外細胞毒性............................................