農(nóng)桿菌介導(dǎo)的辣椒遺傳轉(zhuǎn)化體系優(yōu)化及Chi和Glu基因轉(zhuǎn)化植株的鑒定.pdf

1、Y@3717分糞單位代碼lOVe3甘肅農(nóng)業(yè)大學(xué)學(xué)位論文農(nóng)桿菌介導(dǎo)的辣椒遺傳轉(zhuǎn)化體系優(yōu)化及跏，和Clu基因轉(zhuǎn)化植株的鑒定EstablishmentnfHighEffectAgrobacter『|um—mediatedGeneticTransformationSystemforPepper(LapsicumⅡHH““mL)andldentl姆ingofResistanceonInductionofChitinaseand盧13Glucaaa

2、seGenes李靜雯指導(dǎo)#m#g鱟童皇墼墼f苴煎壅些盤蘭蘭』塑塑!!!學(xué)科專m名#———j量塑』L研究方目—』鰱業(yè)蛐l(wèi)薹墾受塞$請(qǐng)學(xué)＆緞Ⅻ————墮—m女提交日期——望墮蘭互旦立生l一論文答辯日期—墊塑蘭生旦里L(fēng)學(xué)世授予日期——塑盟主旦且』生日一譽(yù)辯垂量擊主席——鹽—壁L—塾—量圃^壁廷熊塑塞星—』塑堂—堂—量～甘肅農(nóng)業(yè)大學(xué)2006屆碩十學(xué)位論文AbstractPepper(CapsicumannuumL)isnotonlyoneoft

3、hemostimportantvegetables，butalsoisoneofmainflavoringSeverelyattackedbymanydiseases，suchasdiseasesfrombacteria，fungal，virusandcelworm，ithasbeingstudiedinordertoimproveitsresistanceBiotechnologybringsUSthenewwaytogettothe

4、target，forexample，bytransferringthedisease—resistantgeneintopepperInrecentyears，manyresearchershavedonepeppergenetictransformation，butlittlesuccessThemainprobleminpeppertransformationisthedifficultyonthelow丹equencyoftran

5、sformationOnthebaseofplantregenerationwhichWasestablished，westudiedsomeconditionsandmethodsofpeppertransformation，andestablishedthehi【ghefficientAgrobacterium—mediatedgenetictransformationsystemforpepperThenwetransferred

6、Chitinasegene(fromtrichodermareesei)andB1，3一glucunasegene(fromtobacco)intopepperandthemoleculardetectionontransgenicplantsshoweditsuccessfulFollowingthesework，weanalyzedtheresistancetophytophthoracapsiciandphenotypicchar

7、acteristicsoftransgenicseedlingsNowthemajorresultsstudiedinthepaperareabstractedasfollows：1EstablishmentofhigheffectgeneticAgrobacteriummediatedgenetictransformationsystemforpepperAndcomparingwithleafexplants，hypocotylee

8、xplantswerelessresistanttokanamycin①50merlekanamycincouldberegardedastheappropriateselectionpressure②ThetimeoftheinfectionwithAtumefaciensisfoundtohaveeffectontransformationrate，and5～8minisrecommended⑨Pre—cultureofexplan

9、tsonthePDfor2daysrbeforeinfectionwithAtumefaciens)，CO—cultivationonPDfor2days(afterinfection)anddelayingselectiononYPD(PDCarb)for2daysarefavourableforthetransformation④500mg／LCarbenicillinrestrainedthegrowthofAgrobacteri

10、umefficiently200m班CefotaximeWaSbenefitforrootinduction2TransgenicpeppersweregotsuccessfullyChitinasemadB1，3GlucanasegenesweretransferredintopepperViahypoeotyleexplartts，and29transgenicseedlingsshowedPCRpositive，thepositi