簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文乙酸鈉和丁酸鈉對奶牛乳腺上皮細(xì)胞及腺泡乳脂合成相關(guān)基因表達(dá)的影響姓名孔慶洋申請學(xué)位級別碩士專業(yè)生物學(xué)；生物化學(xué)與分子生物學(xué)指導(dǎo)教師李慶章20120531ABSTRACTEFFECTOFSODIUMACETATEANDSODIUMBUTYRATEONEXPRESSIONOFGENESRELATEDTOMILKFATSYNTHESISOFDAIRYCOWMAMMARYEPITHELIALCELLSANDACINUSABSTRACTTHEM鋤MARYGLANDOFTHELACTATINGPROCESSIMPORTANTFUNCTIONGENEEXPRESSIONCHANGESISREGULATIONOFMAMMARYGLANDGROWTH，DEVELOPMENT，DIFFERENTIATIONANDMILKSYNTHESIS，SECRETION，TRANSPORTANDOTHERIMPORTANTPHYSIOLOGICALPROCESSBASED．THEREFORE，THEIMPORTANTFUNCTIONOFGENESINMARNMARYGLANDDEVELOPMENTTOIMPROVETHEGENETICPERFORMANCEOFTHECOWS，PROMOTETHEDEVELOPMENTOFCHINA’SDAIRYINDUSTRYHASAPOSITIVEEFFECT．FATTYACIDSYNTHASEFASISAMULTIFUNCTIONALCOMPOSITEENZYME，ISSYNTHESIZEDINVIVOFATPATHWAYOFAKEYENZYMEACETYL．COENZYMEACARBOXYLASE似CCISTHERATELIMITINGENZYMEOFTHEFATTYACIDSYNTHESISPATHWAY,ITCATALYZESTHEFIRSTSTEPREACTIONOFTHESYNTHESISOFFATTYACIDSFA，INTHELIVER，F(xiàn)ATANDBREASTTISSUE，ACESIGNIFICANTLYINFLUENCEDBYPHOSPHORYLATIONORDEPHOSPHORYLATIONREGULATION，ALSOAFFECTEDBYTHEIMPACTOFTHEFAALLOSTERICMECHANISM．DGATLISTHERATE‘LIMITINGENZYMEINTHEPROCESSOFTHETGSYNTHESIS，ASSOCIATEDWITHTHEDAIRYMILKFATCONTENT．PURPOSEOFTHISSTUDYAIMSTOEFFECTOFMILKF缸PRECURSORONMILKFATSYNTHESISGENEEXPRESSION，REVEALTHEMECHANISMOFGENEREGULATIONOFCOWMILKFATSYNTHESIS．INTHEUSEOFINVITROMETHODSTOOBTAINMAMMARYACLNARMODEL．BECOMEAKINDOFERIECTIVESIMPLIFIEDM鋤MARYDEVELOPMENTANDLACTATIONFUNCTIONRESEARCHPROCESSBASICMETHODANDIMPORTANTMEANSTHISEXPERIMENTWITHM黜MARREPITHELIALCELLSANDINVITRODAIRYCOWMAMMARYACINARASRESEARCHOBJECT，USINGFLUORESCENCEQUANTITATIVERTPCRMETHOD，HASDISCUSSEDTHEEXTRANEOUSSOURCEINCREASESODIUMACETATEANDTHESODIUMBUTYRATE，EFFECTOFTHEKEYENZYMEINTHESYNTHESISOFMILKFATTYACIDSINM鋤MARYEPITHELIALCELLSANDMANLMARYACINARMODELOFFAS，ACE，DGATLEXPRESSION，THEUSEOFSEMIAUTOMATICBIOCHEMICALANALYZERBYADDINGVARIOUSNUTRIENTSINCULTURESYSTEMOFGLYCERINTHREEGREASESCONTENTCHANGE，DETERMINEDINVITROREGULATIONOFMILKFATBIOS37NTHESISOFSODIUMACETATECONCENTRATIONOF16MMOL／L；SODIUMBUTYRATECONCENTRATIONOFO．75MMOL／L；BYTRANSFECTIONOFSREBPGENESILENCINGTEST，THERESULTSDISPLAYEDINTWODIMENSIONALCELLSVSTEMANDTHREE．DIMENSIONALACINARSYSTEM，DISTURBSSRE助TOPRESENTTHEINHIBITORYACTIONTO3KINDOFIMPORTANTENZYMEEXPRESSIONS．THISSTUDYFORFURTHERRESEARCHONTHEREGULATIONOFLACTATIONGENESPROVIDESATHEORETICALBASIS．KEYWORDSEPITHELIALCELLS；MAMMARYACINAR；SODIUMACETATE；SODIUMBUTYRATE；MILKFATSYNTHESIS；齜6PIII

簡介：分類號S8523學(xué)校代碼10129UDC11220學(xué)號2009201060TGFΒ1和BFGF對奶牛乳腺細(xì)胞增殖和凋亡的影響EFFECTSOFTGFΒ1BFGFONCELLPROLIFERATIONAPOPTOSISOFBOVINEMAMMARYCELL申請人李瑜學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)基礎(chǔ)獸醫(yī)學(xué)研究方向動物傳染病與免疫病理學(xué)指導(dǎo)教師王鳳龍教授論文提交日期二〇一二年四月EFFECTSOFTGFΒ1BFGFONCELLPROLIFERATIONAPOPTOSISOFBOVINEMAMMARYCELLABSTRACTOBJECTIVEBOVINEMAMMARYEPITHELIALCELLSBOVINEMAMMARYFIBROBLASTSCELLSWERECULTUREDPURIFIEDINTHESTUDYBIOLOGICALACTERISTICSOFTHESECELLSWEREIDENTIFIEDBYOBSERVINGCELLSINCHATELIERTYPEMICROSCOPEDRAWINGCELLULARGROWTHCURVESTOEXPOUNDTHEEFFECTSOFTGFΒ1BFGFONPROLIFERATIONAPOPTOSISOFBOVINEMAMMARYGLULARCELLSMETHODBOVINEMAMMARYCELLSWERECULTUREDBYTISSUEBLOCKMETHODSINGLECELLISOLATIONMETHODONTHEBASISOFTHEDEGREEOFBOVINEMAMMARYEPITHELIALCELLSBOVINEMAMMARYFIBROBLASTSCELLSANCHEDTOASURFACEWEREDIFFERENTTHEBOVINEMAMMARYEPITHELIALCELLSBOVINEMAMMARYFIBROBLASTSCELLSWEREPURIFIEDBYDIGESTIONWITHDIFFERENTCONCENTRATIONSTRYPSINF56PASSAGETIMESTHESHAPEOFCELLWASOBSERVEDBYCHATELIERTYPEMICROSCOPETHESOURCEOFTHECELLSWEREIDENTIEDBYTHEMETHODOFIMMUNOHISTOCHEMISTRYTHEEFFECTSOFTGFΒ1BFGFONPROLIFERATIONAPOPTOSISOFBOVINEMAMMARYGLULARCELLSWEREDETECTEDINTHESTUDYRESULT1THERESULTSHOWTHATTHECELLSHAVETYPICALMPHOLOGICALFEATURESHAVEGOODABILITYOFPROLIFERATION2EXPERIMENTALGROUPSWITHDIFFERENTCONCENTRATIONOFTGFΒ1WERESIGNIFICANTDIFFERENTCOMPAREDWITHTHECONTROLGROUPTHEEFFECTOFINHIBITIONCELLPROLIFERATIONWASENHANCEMENTWITHTHEINCREASEOFTHECONCENTRATIONOFTGFΒ1INBOVINEMAMMARYEPITHELIALCELLSEXPERIMENTALGROUPSWITHDIFFERENTCONCENTRATIONOFTGFΒ1WERESIGNIFICANTDIFFERENCECOMPAREDWITHTHECONTROLGROUPTHEEFFECTOFPROMOTECELLPROLIFERATIONWASENHANCEMENTWITHTHEINCREASEOFTHECONCENTRATIONOFTGFΒ1INBOVINEMAMMARYFIBROBLASTSCELLSBYTHEMETHODOFMTTEXPERIMENTALGROUPSWITHDIFFERENTCONCENTRATIONOFBFGFWERENOTDIFFERENCECOMPAREDWITHTHECONTROLGROUPSINBOVINEMAMMARYEPITHELIALCELLSEXPERIMENTALGROUPWITHDIFFERENTCONCENTRATIONOFBFGFWERESIGNIFICANTDIFFERENCECOMPAREDWITHTHECONTROLGROUPSTHEEFFECTOFPROMOTECELLPROLIFERATIONWASENHANCEMENTWITHTHEINCREASEOFTHECONCENTRATIONOFBFGFINBOVINEMAMMARYFIBROBLASTSCELLSBYTHEMETHODOFMTT3EXPERIMENTALGROUPSWITHDIFFERENTCONCENTRATIONOFTGFΒ1WERESIGNIFICANTDIFFERENCECOMPAREDWITHTHECONTROLGROUPSTHEEFFECTOFPROMOTEAPOPTOSISWASENHANCEMENTWITHTHEINCREASEOFTHECONCENTRATIONOFTGFΒ1INBOVINEMAMMARYEPITHELIALCELLSEXPERIMENTALGROUPSWITHDIFFERENTCONCENTRATIONOFTGFΒ1WERESIGNIFICANTDIFFERENCE

簡介：分類號S8231UDC636學(xué)校代碼10129學(xué)號2009201029十八碳不飽和脂肪酸對乳腺上皮細(xì)胞脂肪代謝的影響及其機(jī)制研究EFFECTSOFC18UNSATURATEDFATTYACIDSONLIPIDMETABOLISMITSMECHANISMSINMAMMARYEPITHELIALCELLS申請人孫曉菊學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)動物營養(yǎng)與飼料科學(xué)研究方向動物營養(yǎng)與畜產(chǎn)品品質(zhì)指導(dǎo)教師敖長金教授論文提交日期二〇一二年六月EFFECTSOFC18UNSATURATEDFATTYACIDSONLIPIDMETABOLISMITSMECHANISMSINMAMMARYEPITHELIALCELLSABSTRACTFATTYACIDISANIMPTANTPARTOFMILKFATMAMMARYEPITHELIALCELLSISTHEDIRECTCOMPONENTSOFFATTYACIDSYNTHESISTRANSPTATIONWEMADEMAMMARYEPITHELIALCELLSASAMODELTOINVESTIGATETHEEFFECTSOFC18UNSATURATEDFATTYACIDSONFATTYACIDMETABOLISMTHEMECHANISMINMAMMARYEPITHELIALCELLSTHEEFFECTSOFC18UNSATURATEDFATTYACIDSONCELLPROLIFERATIONWASTESTEDBYTHEMETHODOFMTTC18UFASTIMULATEDTHEACCUMULATIONOFTRIGLYCERIDETAGINCMECSTESTEDBYOILREDOSTAINUSINGTHERTQPCRTECHNIQUETODETECTTHEEXPRESSIONOFMRNAFCD36FABP3ACACAPPARGSCDTHERESULTSSHEWTHATWITHINGTHEINCREASEOFTHECONCENTRATIONOF50400MLOLEICACIDPROMOTETHEPROLIFERATIONOFCMECSHIGHEROLEICACID800MLINHIBITEDTHEPROLIFERATIONOFCMECSLINOLEICACIDWITHTHECONCENTRATIONOF25100MLPROMOTETHEPROLIFERATIONOFCMECSHOWEVERHIGHTER200MLINHIBITEDLINOLENICACIDWITHTHECONCENTRATIONOF051MLPROMOTETHEPROLIFERATIONOFCMECSHOWEVERHIGHTER28MLINHIBITEDC18UFASTIMULATEDTHEACCUMULATIONOFTRIGLYCERIDETAGINCMECSWHENTHECONCENTRATIONOFOLEICACIDWAS400MLLINOLEICACIDWAS100MLLINOLENICACIDWAS2MLTHEACCUMULATIONSIGNIFICANTLYTHEEXTENTOFOLEICACIDCONCENTRATIONDIDNOTEFFECTTHETRANSCRIOTIONOFCD36BUTHIGHEROLEICACIDDOWNREGULATEDTHEFABP3THETRANIONLEVELOFACACASTEAROYLCOADESATURASESCDGENESWEREINHIBITEDBYOLEICACIDWITHADOSERESPONSEP001OLEICACIDWITHTHECONCENTRATIONOF50ΜMOLLUPREGULATEDTHETRANIONOFPPARGSIGNIFICANTLYLINOLEICACIDUPREGULATEDTHETRANIONOFCD36SIGNIFICANTLYINHIBITEDTHEFABP3ACACASCDSIGNIFICANTLYHIGHERLINOLEICACIDINHIBITEDTHEEXPRESSIONOFMRNAFPPARGLINOLENICACIDUPREGULATEDTHETRANIONOFCD36SIGNIFICANTLYPROMOTETHEEXPRESSIONOFMRNAFACACATHEEXTENTOFLINOLENICACIDCONCENTRATIONDIDNOTEFFECTTHETRANSCRIOTIONOFPPARGSCDKEYWDSOLEICACID；LINOLEICACIDLINOLENICACID；BOVINEMAMMARYEPITHELIALCELLS；METABOLISMOFFATTYAICD

簡介：’關(guān)于學(xué)位論文原創(chuàng)性和使用授權(quán)的聲明本人所呈交的學(xué)位論文，是在導(dǎo)師指導(dǎo)下，獨(dú)立進(jìn)行科學(xué)研究所取得的成果。對在論文研究期間給予指導(dǎo)、幫助和做出重要貢獻(xiàn)的個人或集體，均在文中明確說明。本聲明的法律責(zé)任由本人承擔(dān)。本人完全了解山東農(nóng)業(yè)大學(xué)有關(guān)保留和使用學(xué)位論文的規(guī)定，同意學(xué)校保留和按要求向國家有關(guān)部門或機(jī)構(gòu)送交論文紙質(zhì)本和電子版，允許論文被查閱和借閱。本人授權(quán)山東農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或其他復(fù)制手段保存論文和匯編本學(xué)位論文。保密論文在解密后應(yīng)遵守此規(guī)定。論文作者簽名盟導(dǎo)師簽名J薹籮呀，1日期班噬絲縮略詞FAACSNPEPT2MRNAPEPTLPCRIU’PCRWBDEPC戰(zhàn)SDSSDSPAGEACRBIS“IRISPMSFDDH20TEMEDHIU英文縮略表ENGLISHABBREVIATION英文FREEAMINOACIDSCASEINPEPTIDETRANSPORTERIIMESSENGERRIBONUELEICACIDPEPTIDETRANSPORTERIPOLYMERASECHAINREACTIONREVERSETRANSCRIPTIONPCRWRESTEMBLOTDIETHYLPYROCARBONATEANMAONIUMPERSULFATESODIUMDODECYLSULFATESDSPOLYACRYLAMIDEGELACRYLAMIDEMETHYLENEBISACRYLAMIDETRIHYDROXYMETHYLAMINOMETHANEPHENYLMETHANESULFONYLFLUORIDEDOUBLEDISTILLEDWATERTETMMETHYLDIETHYLAMINEHORSERADISHPEROXIDASE中文游離氨基酸酪蛋白II型肽載體信使核糖核酸I型肽載體多聚酶鏈反應(yīng)反轉(zhuǎn)錄PCR免疫印跡焦碳酸二乙酯過硫酸銨十二烷基磺酸鈉SDS聚丙烯酰胺凝膠丙烯酰胺甲叉雙丙烯酰胺三羥甲基氨基甲烷苯甲基磺酰氟雙蒸水四甲基二乙胺辣根過氧化物酶

簡介：IILLLLLUILLUUUIIIIIY2360033EFFECTSOFGARLICOILONRUMINALBIOHYDROGENATION，MILKFATTYACIDPROFILEANDLIPOGENESISRELATEDGENEEXPRESSIONINMAMMARYGLANDOFGOATSBYZHUZHISUPERVISORPROFZHUWEIYUNADISSERTATIONSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFULFILLMENTOFTHEREQUIREMENTFORPHDDEGREEOFAGRONOMYCOMPLETEDINJANUARY2011COMMENCEMENTINJUNE2011目錄目錄摘要IABSTRACTV縮寫符號一XI引言ⅪII第一篇文獻(xiàn)綜述1第一節(jié)乳脂及共軛亞油酸概況11乳脂肪酸組成12CLA的結(jié)構(gòu)與功能33CLA的形成路徑44生物氫化微生物5第二節(jié)乳脂肪酸組成的營養(yǎng)調(diào)控91改變?nèi)占Z組成911不同粗飼料類型912不同精粗比日糧112添加脂肪酸1221植物脂肪1322魚油或微藻1523共軛亞油酸173調(diào)控乳腺脂肪生成相關(guān)基因的活性194改善瘤胃環(huán)境2241瘤胃PH2342瘤胃調(diào)控劑24第三節(jié)大蒜油的生物學(xué)特性271化學(xué)組成272生物功能303抗菌機(jī)制31第四節(jié)大蒜油在反芻動物上的研究進(jìn)展331大蒜油對蛋白質(zhì)代謝的影響332大蒜油對VFA的影響343大蒜油對甲烷產(chǎn)量的影響35

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文中藥王不留行對奶牛乳腺上皮細(xì)胞核蛋白質(zhì)組學(xué)的影響姓名劉榮申請學(xué)位級別碩士專業(yè)生物學(xué)；生物化學(xué)與分子生物學(xué)指導(dǎo)教師高學(xué)軍20120530ABSTRACT1H●●●●一●RROTEOMLCLDENTLTLCATLONOTDLRENTIALLYEXPRESSEDNUCLEARPROTEINSINVACCARIASEGETALISTREATEDDAIRYCOWMAMMARYEPITHELIALCELLS，一ABSTRACTSTUDIESOFTHEREGULATIONOF吐1EDAIRYCOWLACTATIONTOSOLVETHEPROBLEMSASLOWMILKYIELDSANDPOORQUALITYMILK，WHICHRESTRICTTHEDEVELOPMENTOFCHINADAIRYCOWCULTIVATION，HAVEIMPORTANTTHEORETICALANDPRACTICALSIGNIFICANCEVACCARIAESEGETALISISONEOFTHEMOSTWIDELYUSEDCHINESETRADITIONALHERBSTOPROMOTE1ACTATION；ITHASBEENWIDELYAPPLIEDINCLINICALANDANIMALHUSBANDRYITMAYHAVEESTROGENACTIVITYWHICHCANACTIVATELACTATIONSIGNALWAYANDREGULATETHESYNTHESISOFTHEMILKPROTEIN，LACTOSEANDMILKFAT，ANDTHENINCREASESLACTATIONBUT，THEMECHANISMOFVACCARIAESEGETALISPROMOTINGLACTATIONISNOTCLEAR；ESPECIALLYHOWTOREGULATEGENETICEXPRESSIONOFLACTOGENESISHASNOTBEENREPORTEDINTHEEXPERIMENTS，CASY％TECHNOLOGYWASUSEDTOSTUDYTHEPROLIFERATIONANDVIABILITYOFTHEDAIRYCOWMAMMARYGLANDEPITHELIALCELLSDCMECSATDIFFERENTTIMEAFTERVACCARIAESEGETALISTREATMENTS；PROTEINEXPRESSIONPROFILESWEREANALYZEDBY2DECOUPLEDWITHⅣ姨LDIQTOFMS／MSANALYSIS；ANDSUCHALTERATIONSWEREFURTHERCONFIRMEDBYRTPCRTHERESULTSSHOWEDTHAT④ITWASPROVEDTHATVACCARIAESEGETALISCOULDINCREASETHEPROLIFERATIONANDVIABILITYOFPRIMARYCULTUREDDCMECSSIGNIFICANTLYP005，ANDAT24HWASTHEBEST②A2DESEPARATIONSYSTEMFORPROTEOMEOFNUCLEUARPROTEINSOFDCⅣ匣CSWASSUCCESSFULLYESTABLISHED⑧FIVEPROTEINS，PROTEINSETHYLMALONICENCEPHALOPATHYIETHE1，VESICLEAMINETRANSPORTPROTEIN1HOMOLOGVATL，PARKINSONDISEASEPROTEIN7HOMOLOGPROTEINDJ1，PROTEASOMESUBUNITALPHATYPE2PSMA2ANDSULⅥOACTIVATINGENZYMESUBUNITISAEL，DISPLAYEDSIGNIFICANTEXPRESSIONCHANGESUPONTREATMENTOFTHEWATERDECOCTIONOFVACCARIAESEGETAL，ANDSUCHALTERATIONSWEREFURTHERCONFIRMEDBYQRTPCR④FIVEGENESWEREDIFFERENTEXPRESSEDINTHESETHREEDAIRYCOWSOFLACTATIONLEVELTHEHIGHESTEXPRESSIONLEVELWASINTISSUESOFHIGHYIELDINGDAIRYCOWS，F(xiàn)OLLOWEDBYLOWYIELDINGCOWS，THELOWESTWEREPERINATALCOWSTHERESULTSCOULDBEDRAWNFROMTHEABOVETHESEFIVEGENESASSOCIATEDWITHLACTATIONINCONCLUSION，VACCARIAESEGETALISCANINCREASESPROLIFERATIONOFDCMECSSIGNIFICANTLYITISTHEFIRSTTIMEBYPROTEOMICSMETHODSTOILLUSTRATETHEMECHANISMOFVACCARIAESEGETALISONTHEDCMECS，ANDREVEALTHEPROTEOMICSBASESOFTHEDCMECSWITHSEMENVACCARIA，F(xiàn)URTHERTHESPECIFICMECHANISMOFEACHPROTEINNEEDSTOBESTUDIEDII

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文催乳素、雌激素和胰島素對奶牛乳腺上皮細(xì)胞中瘦素及其受體的影響姓名李冬輝申請學(xué)位級別碩士專業(yè)生物學(xué)；生物化學(xué)與分子生物學(xué)指導(dǎo)教師李慶章20120530INFLUENCEOFPROLACTIN，ESTROGENANDINSULINONLEPTINOFMAMMARYEPITHELIALCELLSINDAIRYCOWABSTRACTLACTATIONOFMAMMARYGLANDSISTHERESULTOFCOREGULATEDBYMAMMOGENESISTOLDHORMONESHORMONESPLAYANIMPORTANTROLEINTHEPROCESSOFLACTATIONINORDERTOUNDERSTANDTHERELATIONOFLEPTINANDEXOGENOUSHORMONESANDRESEARCHTHENEWWAYTECHNIQUESANDMETHODOFARTIFICIALCONTROLMILKYIELDANDQUALITATIVE，ANDPROVIDETHERATIONALEANDTESTFOUNDATION，INTHISEXPERIMENT。HOLSTEINCOWSWEREUSEDASMATERIALS，COWMAMMARYEPITHELIALCELLSWERECULTUREDINVITROANDTREATEDWITHPROLACTIN，ESTROGENANDINSULINTHENLEPTINANDITSACCEPTOREXPRESSEDBYTHOSECELLSANDTHECHANGEOFLACTATIONOFTHOSECELLSWEREOBSERVEDANDANALYZEDBYLASERSCANNINGCONFOCALMICROSCOPY，WESTEMBLOTINGANDREALTIMEPCRRESULTOFTHISRESEARCHINDICATED，THECOWMAMMARYEPITHELIALCELLOFLACTATIONPERIODCANBEOBTAINEDBYTISSUECULTIVATIONTHEOBSERVERESULTOFLASERSCANNINGCONFOCALMICROSCOPYINDICATED。THEEXPRESSIONOFLEPTINANDITSACCEPTORWERELOCATEDONMAMMARYEPITHELIALCELL，EXTRACELLULARMATRIXANDTHEMEMBRANEOFMAMMARYEPITHELIALCELLRESPECTIVELYTHERESULTOFWESTERNBLOTINGANALYSISINDICATED，WHENCOWMAMMARYGLANDEPITHELIALCELLSWERETREATEDWITHPROLACTINANDESTROGEN，THEEXPRESSIONOFLEPTINANDITSACCEPTORBYTHESECELLSWEREROSESIGNIFICANTPO05WASOCCURREDWHENTHESECELLSWERETREATEDWITHINSULINTHISRESULTWASCONSISTENTWITHTHERESULTOFWESTEMBLOTINGANALYSISTHESECRETIONOFLACTOSE，3CASEINANDTRIGLYCERIDEBYCOWMAMMARYEPITHELIALCELLTREATEDWITHPROLACTIN，ESTROGENANDINSULINRESPECTIVELYWERETESTEDBYLIGHEFFICIENCYLIQUIDCHROMATOGRAPHYANDTRIGLYCERIDEKITRESPECTIVELYTHERESULTSINDICATEDTHESECRETIONOFLACTOSEBYCOWMAMMARYEPITHELIALCELLWEREROSESIGNIFICANT伊O05WHENTREATESTROGENANDINSULINTHESECRETIONOF3CASEINBYCOWMAMMARYGLANDEPITHELIALCELLWEREROSESIGNIFICANT儼O05INALLTHREETREATMENTGROUPSKEYWORDSCOWMAMMARYEPITHELIALCELL；PROLACTIN；ESTROGEN；INSULIN；LEPTIN；LEPTINACCEPTOR；CANDIDATELIDONGHUISPECIALITYBIOCHEMISTRYANDMOLECULARBIOLOGYSUPERVISORPROFLIQINGZHANGIII

簡介：西北農(nóng)林科技大學(xué)碩士學(xué)位論文西農(nóng)薩能奶山羊Β酪蛋白基因5′側(cè)序列克隆和分析及乳腺特異性表達(dá)載體的構(gòu)建姓名朱婧申請學(xué)位級別碩士專業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師竇忠英20050601CLONINGANDANALYSISOFBETACASE玎NGENE57FLANKINGSEQUENCEFROMⅪNONGSAANENDAIRYGOATANDCONSTRUCTIONOFMAMMARYGLANDSPECIFICEXPRESSIONALVECTORABSTRACTMAMMARYGLANDBIOREACTORISAUSEFULBIOLOGICALSYSTEMWHICHEXPRESSESFOREIGNGENESINANIMALMAMMARYGLANDANDPRODUCESFUNCTIONALLYPHARMACEUTICALPROTEININMILKTHISPRODUCTIONROUTEISAPPEALINGFORIT’SADVANTAGES，SUCHASTHESIMPLICITYOFACCESSTOTHEEXPRESSEDPROTEIN，THEHIGHPRODUCTIONOFTHEMAMMARYGLAND，ASWELLASTHECAPABILITIESTOPERFORMPOSTTRANSLATIONALPROCESSINGASANALTERNATIVEOFCELLCUKURE，ITISANEWBIOTECHNOLOGYINFACT，SUCCESSFULGENETRANSFERHASBEENDESCRIBEDFORALLTHEMAJORCOMMERCIALLIVESTOCKSPECIESINCLUDINGGOATS，PIGS，ANDCOWSINTHEPRESENTSTUDYSPECIFICPRIMERSFORGOAT13CASEINGENEPROMOTERHADBEENDESIGNEDANDSYNTHESIZEDACCORDINGTOTHEPUBLISHEDNUCLEICACIDSEQUENCEOFGOATCOMPLETE3CASEINGENEXINONGSAANENDAIRYGOAT13CASEIN5’FLANKINGSEQUENCEWASOBTAINEDBYLONGANDACCURATEPCRTHEPCRPRODUCTWASTHENCLONEDINTOPMDL8TVECTORANDSEQUENCEDTHECLONEDFRAGMENTCOVEREDTHE5’FLANKINGSEQUENCEOFTHEGOAT13CASEINGENEFROM4359TO2106BPBIOINFORMATICANALYSISINDICATEDTHATPUTATIVEPROMOTERSEQUENCEOFTHECLONEDXINONGSAANENDAIRYGOAT13CASEIN57FLANKINGSEQUENCESHARED96％98％HOMOLOGYWITHOTHERGOAT3CASEINGENEPROMOTERSXINONGSAANENDAIRYGOATPCASEIN5’FLANKINGSEQUENCEREGIONCONTAINEDPROMOTERSEQUENCESANDPUTATIVERECOGNITIONSITESFORSEVERALTRANSCRIPTIONALFACTORS，INCLUDINGHOXF，OCTL，APL，CEBP，STAT，NFKB，GR，ER，PR，ETSANDTHEINTRON1，EXON1ANDEXON2OFTHEGOAT3CASEINGENERECONSTRUCTEDREPORTERPLASMIDPGL3／B65WASESTABLISHEDBYINSERTING6，465NUCLEOTIDESFROMTHEPROMOTERREGIONINTOTHELUCIFERASEREPORTERVECTORPGL3BASICFINALLY，THEPGL3／B65WASTRANSFECTEDINVITROVIALIPOSOMESINTOTHEPRIMARYGOATMAMMARYEPITHELIALCELLTRANSIENTTRANSFECFIONASSAYSHOWEDTHEPCASEIN5’FLANKINGSEQUENCEREGIONHADPROMOTERFUNCTION，ANDITRESPONDEDTOHORMONALTREATMENTTHEN，THEPCASEIN5’FLANKINGSEQUENCEWASINSTEADOFTHEPCMVPROMOTERINPCDNA31VECTORTOCONSTRUCTIONOFAMAMMARYGLANDSPECIFICEXPRESSIONALVECTORPCDNA3165ANDTHENHBMP2CDNAWASCLONEDINTOPCDNA3165THEPRIMARYGOATMAMMARYEPITHELIALCELLSMECTRANSIENTLYTRANSFECTEDWITHVECTORPCDNA31，

簡介：Ⅲ州0洲洲㈣I㈣Y2438225隸劫大訾臨床醫(yī)學(xué)碩士學(xué)位論文乳腺癌CXCRL的表達(dá)特征及其與新輔助化療療效的相關(guān)性及臨床意義研究生姓名篚纓登導(dǎo)師姓名型I丞忠申請學(xué)位類別監(jiān)鏖醫(yī)生亟學(xué)位授予單位盔畝塞堂專業(yè)名稱鹽抖雯研究方向萱道處抖雯答辯委員會主席殛蛭論文答辯同期2Q】3生Q縣22旦學(xué)位授予日期2Q13生縣旦評閱人王淫壬張堊男一2013年05月27臼EXPRESSIONCHARACTERISTICSOFCXCRLINBREASTCARCINOMAANDTHERELEVANCEWITHTHEEFFECTOFNEOADIUVANTCHEMOTHERAPY1|ILLLLLLLLLLLLLLLLL111ADISSERTATIONSUBMITTEDTOSOUTHEASTUNIVERSITYFORTHECLINICDEGREEOFMASTEROFMEDICINEBYXUEMIAOQUNSUPERVISEDBYPROFESSORYAOYONGZHONGSCHOOLOFMEDICINESOUTHEASTUNIVERSITYMAY2013

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文蛋氨酸對奶牛乳腺上皮細(xì)胞亞細(xì)胞器蛋白質(zhì)組學(xué)影響姓名王佳麗申請學(xué)位級別碩士專業(yè)基礎(chǔ)獸醫(yī)學(xué)；動物生物化學(xué)指導(dǎo)教師高學(xué)軍20120530ABSTRACTABSTRACTLOWMILKYIELDANDMILKPROTEINCONTENTHAVEBEENRESTRICTINGTHEDEVELOPMENTOFCHINESEDAIRYINDUSTRYSTUDYOFLACTATIONREGULATIONMECHANISMONDAIRYCOWSHAVEBOTHTHEORETICALANDPRACTICALSIGNIFICANCEMETHIONINEISANIMPORTANTPRECURSOROFMILKPROTEINSYNTHESIS，PREVIOUSSTUDIESMAINLYFOCUSEDONTHEIMPACTOFMETHIONINEONDAIRYPRODUCTIONPERFORMANCE，WHILETHEEFFECTSOFLACTATIONREGULATIONONDAIRYCOWMAMMARYGLANDEPITHELIALCELLSDCMECSREPOORLYRESEARCHEDITISNEEDEDTOSTUDYTHERELATIONSHIPBETWEENMETHIONINEANDMILKPROTEINSYNTHESISASWELLASLACTATIONABILITYOFMAMMARYGLAND，UNDERSTANDTHEMOLECULARMECHANISMATPROTEINLEVELANDFIND廿1ECMCIALPROTEINSWHICHCORRELATEWITHLACTATINGINTHISSTUDYINVITROCULTUREDDCMECSMODELWASSUCCESSFULLYESTABLISHEDEFFECTSOFMETHIONINEONPROLIFERATIONOFDCMECSWEREEVALUATEDBYHPLC，RTPCRCASYANDTRIGLYCERIDEREAGENTTODETERMINETHEOPTIMUMCONCENTRATIONOFMETHIONINE，TWODIMENSIONALELECTROPHORESISWASAPPLIEDTOANALYZESUBCELLULARPROTEOMICSOFHEALTHYDCMECSANDMETHIONINE’SUPPLEMENTEDDCMECSCULTUREDINVITROTHEIMAGEMASTER2DESOFTWAREWASUSEDTOCHARACTERIZEDIFFERENTPROTEINSANDDIFFERENTIALLYEXPRESSEDPROTEINSPOTSWEREIDENTIFIEDBYMALDITOFMSTHEMAINRESULTSOFTHEPRESENTSTUDYWEREASFOLLOWS1、METHIONINEWASADDEDTOTHEDMEM／F12CULTUREMEDIUMATCONCENTRATIONSOF0，02，O4，08，12AND16MMOL／LCULTUREPERIODWAS24HTHERESULTSSHOWEDTHAT12MMOI／LMETHIONINESIGNIFICANTLYIMPROVEDTHEPROLIFERATIONOFDCMECSP001，ITSTHEBESTCONCENTRATIONFORDCMECSCULTUREDINVITRO2WECONSTRUCTEDTHETWODIMENSIONALGELELECTROPHORESISPROFILESFORSUBCELLULARPROTEINSOFDCMECSTHE2DEPROFILESHADBOTHGOODRESOLUTIONANDREPRODUCIBILITYDIFFERENTIALLVEXPRESSEDPROTEINSPOTSINDCMECSBETWEENCONTROLANDMETHIONINESUPPLEMENTEDWERESCREENEDANDIDENTIFIEDBYMASSSPECTRUM5UPREGULATEDPROTEINSPOTSINMETHIONINESUPPLEMENTEDDCMECSGROUPWERESCREENEDOUTBYIMAGEMASTER60SOMVARETHESESPOTSWEREIDENTIFIEDBYMALDITOFMSANDSEARCHEDINTHENCBINRANDEBIDATABASES，INCLUDINGC啪ITILLEACETYLTRANSFERASE，DIHYDROPYRIMIDINASERELATEDPROTEIN2，OMITHINEAMINOTRANSFERASENADHDEHYDROGENASEUBIQUINONE】FLAVOPROTEIN2，APOLIPOPROTEINA～I(xiàn)BINDINGPROTEINTHERESULTSSHOWEDTHESEDIFFERENTIALLYEXPRESSEDPROTEINSWEREMAINLYINVOLVEDINAMINOACIDTRANSPORT，ENERGYMETABOLISMANDCYTOSKELETONCOMPOSITION，ETC3THE2DERESULTSWEREVERIFIEDONTHEMRNALEVELBYRTPCRTHESEDIFFERENTIALLVEXPRESSEDPROTEINSHADTHEHIGHESTEXPRESSIONLEVELINHIGHLACTATIONLEVELDAIRYCOWS，THESECONDLVEXPRESSIONLEVELINLOWLACTATIONLEVELDAIRYCOWS，ANDTHELOWESTEXPRESSIONLEVELINDRYPERIODCOWSTHEDIFFERENTIALLYDISPLAYEDPROTEINSBETWEENCONTROLANDMETHIONINESUPPLEMENTEDDCMECSAREUSEFULFORLACTATINGMECHANISMRESEARCHIII

簡介：分類號S8523學(xué)校代碼10129UDC5912學(xué)號2010301014TNFΑ和IFNΓ及含有五種細(xì)胞因子的奶牛乳汁對奶牛乳腺纖維化影響的研究STUDIESONTHEEFFECTOFEXOGENOUSTNFΑIFNΓBOVINEMILKCONTAININGFIVEKINDSOFCYTOKINESINTHEBOVINEMAMMARYFIBROSIS申請人楊磊學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)基礎(chǔ)獸醫(yī)學(xué)研究方向動物傳染病與免疫病理學(xué)指導(dǎo)教師王鳳龍教授論文提交日期二〇一三年六月MRNA表達(dá)量均比BFGF低含量的乳汁作用高，但對ΑSMAMRNA表達(dá)的抑制作用卻相反。PDGFBB高含量的乳汁作用BMEC，CTGF和COLIΑ1MRNA表達(dá)量均比低含量的乳汁作用高，但對ΑSMAMRNA表達(dá)的抑制作用卻相反；PDGFBB高含量的乳汁作用BMFB，ΑSMAMRNA表達(dá)量均比低含量的乳汁作用高，而對CTGF和COLIΑ1MRNA表達(dá)的影響卻相反。研究結(jié)果表明，兩種外源性細(xì)胞因子及含有細(xì)胞因子的乳汁對奶牛乳腺上皮細(xì)胞和成纖維細(xì)胞中纖維化指標(biāo)的表達(dá)均產(chǎn)生一定的作用，為進(jìn)一步探究乳腺纖維化的發(fā)生機(jī)制奠定了基礎(chǔ)。關(guān)鍵詞奶牛；乳腺；纖維化；轉(zhuǎn)分化；細(xì)胞因子

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文奶牛乳腺上皮細(xì)胞凋亡周期性規(guī)律及機(jī)理姓名肖陽申請學(xué)位級別碩士專業(yè)生物學(xué)；生物化學(xué)與分子生物學(xué)指導(dǎo)教師李慶章20120531ABSTRACTPERIODICITYRULEANDMECHANISMOFAPOPTOSISINDAIRYCOWMAMMARYEPITHELIALCELLSABSTRACTAPOPTOSISISPROGRAMMEDCELLDEATHPROCESS，ISALLESSENTIALPHYSIOLOGICALPROCESSTHATPLAYSACRITICALROLEINDEVELOPMENTANDTISSUEHOMEOSTASISTHEBREASTISARENEWABLETISSUE，WHICHTOGOTHROUGHTHEPROCESSOFCELLPROLIFERATIONANDAPOPTOSISINEACRHPREGNANCYCYCLE，INPARTICULARAFTERWEANING，THEBREASTGETSINTOADEGRADATIONPROCESS，ALONGWITHALARGENUMBEROFMAMMARYEPITHELIALEELLAPOPTOSISBECAUSEAPOPTOSISISANECESSARYSTAGETHATBREASTREALIZEITSIHNCTION，SOITCANBEBETTERTOUNDERSTANDTHECHANGERULEOFBREASTDEVELOPMENTSTRUCTURE，F(xiàn)INDTHEBIOLOGICALCONTROIMETHODSTOREASONABLELYPROLONGLACTATIONANDINCREASEMILKYIELDTHROUGHSYSTEMATICALLYRESEARCHDERIODICRULEANDITSMECHANISMOFAPOPTOSISINMAMMARYGLANDDEVELOPMENTEFFECTIVEREGULATIONOFTHEFUNCTIONOFCOWSATTHEGENETIC1EVEL，ITCANIMPROVEDAIRYCOWYIELD，LMPROVETHECONVERSIONRATEOFNUTRIENTSANDAMELIORATEMILKQUALITYITCALSOCANIMPROVETHEECONOMICE伍CIENCVOFDAIRYFARMINGANDSOLVESOMEMAJORISSUESFACINGTHEFIELDOFDAIRYINDUSTRYINCHINAINTHISSTUDYHOLSTEINDAIRYCOWSWEREUSEDASTHEEXPERIMENTALMATERIALS，TUNELWASUSEDTODETECTTHEAPOPTOSISOFMAMMARYCELL，MAMMAR2Y’GLANDTISSUESOFDIFFERENTDEVELOPMENTALSTAGESWERESLICEDINFROZEN，TODETECTTHEAPOPTOSISSINGLEINDAIRYCOWMAMMARYGLANDTISSUEANDTODETERMINETHEAPOPTOSISRULEATDIFFERENTDEVELOPMENTSTAGESINDAIRYCOWMAMMARYGLANDTISSUEAPPLYINGFLUORESCENCEQUANTITATIVERTPCRANDWESTERNB101TINGTECHNIQUES，THEEXPRESSIONOIASERIESAPOPTOSISGENESANDPROTEINSATDIFFERENTDEVELOPMENTSTAGESINDAIRYCOWMAMMARYGLANDTISSUEWEREDETECTEDINCLUDINGBAXAPOPTOTICPROTEIN，TGFFLL，P53GENE，ANDTOVERIFYEXPRESSIONOFIMPORTANTSIGNALAPOPTOSISRELATEDPROTEIN，INCLU一LINGCASPASE3DOWNSTREAM，BAXUPSTREAMTNFRLUPSTREAMTUNELRESULTSSHOWEDTHAT，INVIRGIN，MAMMARYGLANDDEVELOPEDSOSTABLELYANDMOSTBREAST“SSHEWASTHEFATPADSTRUCTURE，INAPERIODOFISOMETRICGROWTHSIMULTANEOUSLYTHEEXTENTOFAPOPTOSISWASRELATIVELYLOWINPREGNANCYMAMMARYGLANDDEVELOPEDRAPID，WITHALARGENUMBEROFALVEOLUSANDAPOPTOSISWASINCREASEDININITIALPREGNANCY2M，UNDERTHEACTIONOFTHEPIPUITARY，OVARIANANDPLACENTALSECRETIONOFMANYHORMONES，THEDUCTALSYSTEMANDALVEOLUSDEVELOPEDRAPIDLYAPPEAREDASECONDARYAPOPTOSISPEAKINLACTATIONTHESTRUCTUREOFMAMMARYGLANDWASSTABLE。LITTLENEWBORNANDDEGRADATIONOFSTRUCTURALCHANGESANDAPOPTOTICSIGNALWASALMOSTUNDETECT￡LBLELATELACTATIONUNTILINVOLUTION，GLANDALVEOLUSBEGANTOOPENANDGRADUALLYTRANSITEDTOINVOLUTIONALVEOLAREPITHEIALCELLSGRADUALLYBEGANTOUNDERGOAPOPTOSIS，THEAPOPTOTICSIGNALINCREASEDTHANINEARLYLACTATIONININVOLUTION3D，THEAPOPTOSISWASHIGHESTANDREACHEDTHEPEAK，GRREENPOSITIVESIGNALWASALMOSTFULLOFSCREEN，ANDTHENDECTEASEDININVOLUTION30D，THEMAMMARYGLANDWASRECOVEREDTOTHEVIRGINSTATUS，THEAMOUNTOF。APOPTOSISWASALSEDUCED，TOIII

簡介：點(diǎn)擊查看更多乳腺癌HER2異質(zhì)性分析及信號調(diào)控機(jī)制分析.pdf精彩內(nèi)容。

簡介：分類號S8學(xué)校代碼10758密級公開學(xué)號1035020223碩士學(xué)位論文移植骨髓間充質(zhì)干細(xì)胞對乳腺炎模型大鼠乳腺作用的研究RESEARCHONTHATTRANSPLANTINGBONEMARROWMESENCHYMALSTEMCELLSHASANEFFECTONMASTITSISOFMAMMARYMASTITISMODELRATS研究生姓名秦小惠導(dǎo)師姓名及職稱余雄教授合作導(dǎo)師姓名及職稱學(xué)位門類級別農(nóng)學(xué)碩士專業(yè)名稱動物營養(yǎng)研究方向反芻動物營養(yǎng)與飼料科學(xué)所在學(xué)院動物科學(xué)學(xué)院新疆烏魯木齊二○一三年六月獨(dú)創(chuàng)性聲明本人聲明所呈交的論文是我個人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得新疆農(nóng)業(yè)大學(xué)或其他教育單位的學(xué)位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示了謝意。研究生簽名時間年月日關(guān)于學(xué)位論文使用授權(quán)的說明本人完全了解新疆農(nóng)業(yè)大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定，即新疆農(nóng)業(yè)大學(xué)有權(quán)保留并向國家有關(guān)部門或機(jī)構(gòu)送交論文的復(fù)印件和電子文檔，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文，允許論文被查閱和借閱。本人授權(quán)新疆農(nóng)業(yè)大學(xué)將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以公布（包括刊登）論文的全部或部分內(nèi)容。BMSCS組血清中MDA水平于3D和7D時顯著低于對照組和抗生素組P005。病理切片觀察發(fā)現(xiàn)，灌注內(nèi)毒素前大鼠乳腺組織無異常，腺泡上皮細(xì)胞排列整齊；注射內(nèi)毒素24H后乳腺腺泡結(jié)構(gòu)破壞嚴(yán)重，局部崩塌，乳腺上皮細(xì)胞脫落、壞死，腺泡腔和間質(zhì)內(nèi)有大量脫落上皮細(xì)胞和中性粒細(xì)胞浸潤，乳腺小葉間隔增寬；治療后各組乳腺組織逐漸恢復(fù)，治療各時間點(diǎn)移植BMSCS組和抗生素組大鼠乳腺恢復(fù)效果均比生理鹽水組明顯，試驗(yàn)7D時移植BMSCS組已恢復(fù)到正常水平。由以上結(jié)果得出結(jié)論①本試驗(yàn)成功的建立了大鼠乳腺炎模型；②移植BMSCS能降低乳腺炎大鼠血清中TNFΑ、IL1Β、IL8水平；③移植BMSCS可以提高乳腺炎大鼠血清中免疫球蛋白IGM、IGA、IGG水平，增強(qiáng)機(jī)體的免疫力；④BMSCS能顯著降低乳腺炎大鼠血清中MDA水平，提高血清中SOD和CAT活性，從而提高機(jī)體清除自由基的能力；⑤BMSCS能降低乳腺炎大鼠血清中急性期蛋白水平；⑥BMSCS能降低血清中LDH、NAGASE、MPO等乳腺炎相關(guān)酶活性，促進(jìn)受損乳腺組織的修復(fù)。關(guān)鍵詞骨髓間充質(zhì)干細(xì)胞；乳腺炎；炎性因子；免疫球蛋白；自由基；抗氧化酶RESEARCHONTHATTRANSPLANTINGBONEMARROWMESENCHYMALSTEMCELLSHASANEFFECTONMASTITSISOFMAMMARYMASTITISMODELRATSABSTRACTINORDERTOSTUDYTHEEFFECTONBREASTREPAIRIONOFBREASTMASTITISMODELRATSTHATAFTERTRANSPLANTATINGBONEMARROWMESENCHYMALSTEMCELLS,BMSCSWEREINJECTEDTORATS’BREASTBASE,ANDEXPLORETHEREPAIREDEFFECTSONMAMMARYGLANDTHATBMSCSHAVEANDPOSSIBLEMECHANISMOFACTIONBYDETECTINGTHELEVELSOFSERUMINFLAMMATORYCYTOKINESTNFΑ,IL1Β,IL2,IL6,IL8,IMMUNOGLOBULINIGM,IGA,IGG,FREERADICALMDA,THEACTIVITYOFANTIOXIDANTENZYMESSOD,CATANDGSHPX,ENZYMES,ANDOBSERVATEDTISSUESECTION,,THUSPROVIDINGANEWMETHODFORTHETREATMENTOFMASTITIS,ANDLAYINGANTHEORETICALBASISONTHERESEARCHFORBMSCSREPAIRINGMECHANISM20SDFEMALERATSWERESELECTEDATEARLYTIMEOFTHETEST,THEYWEREDIVIDEDINTOTWOGROUPSEXPERIMENTALGROUPANDCONTROLGROUP,N1010RATSWERESELECTEDRANDOMLYFORESTABLISHINGMASTITISMODELBYINFUSINGENDOTOXINTHROUGHNIPPLETUBE,ANDANOTHER10RATSWEREPERFUSEDSALINEINTHESAMEWAYAFTER24HOFINFUSINGENDOTOXIN,THERATSWEREALLKILLED,MAMMARYGLANDTISSUESWERECOLLECTEDIMMEDIATELY,FIXEDFORTISSUESECTION,CARDIACBLOODWASCOLLECTEDFROMTHEDEADBODIESANDPRESERVEDFORDETECTINGTHELEVELSOFTNFΑ,IL1Β,IL,IL8,HP,SAA,CRP,MDA,LDH,NAGASETHERESULTSSHOWEDTHATTNFΑ,IL1Β,IL6,IL1,IL8,HP,SAA,CRP,MDA,LDH,NAGASEINCREASEDSIGNIFICANTLYP005,SOD,GSHPXDECREASEDSIGNIFICANTLYP005INCONTROLGROUP,RATS’BREASTTISSUESHAVENOOBVIOUSCHANGESAFTERINFUSINGENDOTOXIN,RATSINEXPERIMENTALGROUPDEPRESSED,ANOREXIA,MAMMARYGLANDSWELLEDPATHOLOGICALSECTIONSHOWEDTHATMAMMARYGLANDFLOCCULUSBECOMEWIDEN,BREASTTISSUECOLLAPSED,ANDWEREINFILTRATEDBYALOTOFFALLENMAMMARYGLANDEPITHELIALCELLSANDINFLAMMATORYCELLS,ANDITPROVEDTHATMASTITISMODELHADBEENESTABLISHEDSUCCESSFULLY

簡介：分類號學(xué)號2009004／／L丫L／I／IZ／HL／14T／／L／14／LL／L／。／LLL／19LLLLH8IHLL6／L／／LL／泰山壓虧弦碩士學(xué)位論文論文題目應(yīng)用蛋白質(zhì)組學(xué)方法篩選乳腺癌相關(guān)蛋白質(zhì)作者姓名侯巍巍學(xué)科、專業(yè)病理學(xué)與病理生理學(xué)。學(xué)位類型科研學(xué)位型研究方向腫瘤方向指導(dǎo)教師侯剛教授入學(xué)時間2010年9月論文工作起止時間2011年10月2013年2月應(yīng)用蛋白質(zhì)組學(xué)方法篩選乳腺癌相關(guān)蛋白質(zhì)研究生侯巍巍專業(yè)病理學(xué)與病理生理學(xué)導(dǎo)師侯剛教授中文摘要目的本實(shí)驗(yàn)運(yùn)用雙向凝膠電泳TWODIMENSIONALGELELECTROPHORESIS，2DE技術(shù)、質(zhì)譜分析MASSSPECTROMETRY，MS技術(shù)以及生物學(xué)信息數(shù)據(jù)庫搜索等方法，篩選乳腺癌組織與周圍正常乳腺組織之間的差異蛋白，通過比較這些差異表達(dá)蛋白譜，尋找和鑒定與乳腺癌相關(guān)的蛋白質(zhì)，深入了解這些蛋白質(zhì)的結(jié)構(gòu)和功能，篩選出與乳腺癌診斷及預(yù)后相關(guān)的特異性標(biāo)志物并為進(jìn)一步研究奠定基礎(chǔ)。方法選取新鮮乳腺浸潤性導(dǎo)管癌組織及癌旁30CM以外的周圍乳腺正常組織經(jīng)病理證實(shí)30例進(jìn)行雙向凝膠電泳，得到的差異蛋白點(diǎn)用IMAGEMASTER2DPLATINUM50圖像分析軟件進(jìn)行分析，顯示蛋白點(diǎn)強(qiáng)度比值20，說明蛋白點(diǎn)具有表達(dá)差異。運(yùn)用基質(zhì)輔助激光解吸電離時間飛行質(zhì)譜分析MATRIXASSISTEDLASERDESORPTIONIONIZATIONTIMEOFFLIGHTMASSSPECTROMETRY，MALDITOFMS對所篩選的差異蛋白點(diǎn)做質(zhì)譜鑒定；根據(jù)檢索軟件MASCOT計(jì)算得到蛋白的分值，分值達(dá)到70分以上認(rèn)為這個蛋白質(zhì)鑒定可靠；將質(zhì)譜分析得到的數(shù)據(jù)輸入NCBI數(shù)據(jù)庫來獲得蛋白質(zhì)的信息。結(jié)果利用IMAGEMASTER2DPLATINUM50凝膠圖象分析軟件對兩樣本總蛋白進(jìn)行分析，尋找差異蛋白位點(diǎn)，得出結(jié)果浸潤性導(dǎo)管癌組織總共有L0404L5個蛋白位點(diǎn)，癌旁正常乳腺組織共得到了100317個蛋白位點(diǎn)。根據(jù)IMAGEMASTER2DPLATINUM50凝膠圖象分析系統(tǒng)分析后所得到的差異蛋白數(shù)