水稻化學(xué)誘導(dǎo)啟動子的功能研究及其應(yīng)用相關(guān)抗病基因的克隆和表達(dá)分析.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文水稻化學(xué)誘導(dǎo)啟動子的功能研究及其應(yīng)用相關(guān)抗病基因的克隆和表達(dá)分析姓名：王維平申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：王喜萍曹凱鳴20030501復(fù)旦大學(xué)碩士學(xué)位論文FunctionalstudyonchemicalinduciblepromoterinriceandCloningandexpressionanalysisofresistance—relatedgeneRCOllAbstractWitht

2、herapiddevelopmentofbasicbiologyresearchandbiotechnology，howtoutilizefunctionalgenesbymeansoftransgenictechniquestoimprovecrop’squality，yieldandstresstolerancesafelyandemcientlyisnowbecomingagreattaskGeneexpressionsinviv

3、oarestrictlyregulatedundernormalconditionsConstitutivepromotersarenowwidelyusedinbiotechnologytocontrolexogenousgenes’expression，whichwastesenergysources，influencesthecrop’sregulardevelopment，andbringsaboutproblemofbiosa

4、fetyUsinginduciblepromoterstocontroltargetgenes’expressiontimelycanovercomethoseconstitutivepromoters’weaknesses，providepowerfultoolsforthebasicresearchofplantmolecularbiologyandbroadapplicationoftransgenictechniquesOurp

5、rojectfocusesonisolationofchemicalinduciblepromoterinrice，whichisavail曲leinfutureapplicationMeanwhile，weisolatedresistance—relatedgeneR刪inrice，analyzeditsinduciblecharacteristics，toprovidegeneresourcefortheconstructionan

6、dapplicationofchemicalinduciblediseaseresistancesysteminrice。BasedonthesequenceoftheOryzasativagene樹Gwhoseexpressionisunderthecontroloftheherbicidechlorsulfitron，variousfragmentsofthe5’一upstreamof朋Gpromoterwereisolatedan

7、dconnectedteportergene(GL7研，thenclonedintoplanttransgenicvectorTheregulatorycharacteristicofthepromoterwasstudiedinonionepidermictransientexpressionsystemandmodeiplantArabidopsisthalianaviaparticlebombardmentandagrobacte

8、riummediatedtransformationrespectivelyOntheotherhand，basedontheresultofblastanalysis，weisolatedanovelgenei11rice，sho、vinglli曲homologuetothediseaseresistancegeneC洲inArabidopsisthal／atladesignatedasRCoIinelltheinducibleexp

9、ressions0iRCoiibplanthormonesuchasSAJAABA，ethylenewerefurtheranalyzedbysemi—quantitativeRTPCR，showin2thatitsexpressionwasinducedbyJAandABA，whichindicatesRCoIjworksinJAandABAinduceddisease—resistancepathwaysCOIicanbeuseda