中草藥體外抗雞新城疫病毒活性及作用方式研究.pdf

1、國(guó)內(nèi)圖書(shū)分類號(hào)國(guó)際圖節(jié)分類號(hào)農(nóng)學(xué)碩士學(xué)位論文785307研究生學(xué)號(hào)20006119中草藥體外抗雞新城疫病毒活性及作用方式研究研究生導(dǎo)師申請(qǐng)學(xué)位級(jí)別學(xué)科、專業(yè)所在單位答辯日期授予學(xué)位單位姜宗敏張秀英(教授)農(nóng)學(xué)碩士基礎(chǔ)獸醫(yī)學(xué)東北農(nóng)業(yè)大學(xué)2004年11月東北農(nóng)業(yè)大學(xué)東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文StudiesofherbsonantiviralactivityandactionsofNDV—invitroAbstractNewcastleDi

2、sease，alsoknownasAsiachicken’splagueorfakechicken’splague，cancauseanacuteandhighlyinfectiousdiseasebyNewcastleDiseaseVirus(NDV)HighmortalityrateseriouslyendangeredthepoultryindustryChemicaldrugs，whichhavebeenused，don’two

3、rkwellFurthermore，becauseoflongsyntheticalcycle，highCost，greatsideeffectsandimperfectimmunitypeopleturnedtoChineseherbsHowtodevelopdrugsthatarehighefficient，lessresidualandlowresistanthasbecomeanemergentproblemtobesolved

4、Byorthogonalexperimentaldesign，theantiNDVactivityoftraditionalsixChineseherbsFoliumisatidis，Radixisatidis，Herbahouttuyniae，Patriniaviilosajuss，Rhizomapolygonicuspidati，Radixsophoraeflavescentiswassystematicallyinvestigat

5、edinchickenembryosThenscientificcombinationwascarriedoutMoreovertheactionofChineseherbalmedicine刪nstNDVwasstudiedinchickenembryofibroblasteell(CEF)invitroThebestcompositewasFoliumisatidis，Herbahouttuyniae，Rhizomepolygonc

6、uspidati，Patriniavillosa／ussandRadixsophoraeflavescentisThreeways，thefirstwasaddingmedicineandvirusafteractedforawhile，thesecondwasinoculatingvirusafteraddingmedicine，thethirdwasinoculatingvirusbeforeaddingmedicineweread

7、optedinchickenembryosandCEFTheanti—NDVactionoftraditionalsixChineseherbsandthebettercompositewassystematicallyinvestigatedTheresultofsinglemedicineinchickenembryosindicatedthatthemaximumnontoxicconcentrationofFoliumisati

8、dis，Radixisatidis，Herbahouttuyniae，PatriniavillosajusswascorazspgndingtoloO∥mIofrawmedicineThatofRadixsophoraeflavescentiswascorrespondingto0809／mlandtheRhizomapolygonicuspidatiwascorrespondingtoO309／mlInthefirstway，theo

9、therfivemedicinesexceptRadixsophoraeflavescentisagainstNDVwasbetterInthesecondway，theeffectofHerbahouttuyniae，Patriniaviilosa]uss，RhizomapolygonicuspidatiandRadixisatidistoNDVwasbetterbutRadixsophoraeflavescentisandFoliu

10、misatidishadlittleefiect1nthethirdway，RadixisatidisHerbahouttuyniae，PatriniavillosajussweremoreeffectivethanFoliumisatidis，RhizomapolygonicuspidateInshort，theeffectsofRadixisatidis，PatriniaviltosajussandHerbahouttuyniaeo

11、nNDVwerehigherThefirstofthreemoresatisfactorycomplexcompositesobtainedfromtheexperimentwasFoliumisatidisHerbahouttuyniaeRhizoraapolygonicuspidati，PatriniavillosajussandRadixsophoraeflavescentisThesecondwasFoliumisatidis，