小白菊內(nèi)酯協(xié)同低劑量奧沙利鉑的抗腫瘤作用及機制.pdf

1、浙江大學(xué)醫(yī)學(xué)院碩士學(xué)位論文小白菊內(nèi)酯協(xié)同低劑量奧沙利鉑的抗腫瘤作用及機制姓名：徐濤申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)（呼吸病學(xué)）指導(dǎo)教師：周建英20091020浙江大學(xué)碩上學(xué)位論文摘要———————二————————一———————————————————————————————————————‘—————————————————————————————————一Sesquiterpenelactoneparthenolidemarkedl

2、yenhancessensitivityofhumanA549cellstolowdoseoxaliplatinPhMCandidate：TaoXUSupervisor：Prof；JianYingZhouAbstractBackgroundandObjective：AberrantactivationofNF1(BhasbeenproposedasthemajorcauseofchemoresistanceinlungcancerLow

3、dosechemotherapeuticagentswithlimitedtoxicityachievingprofoundlyenhancedefficacybyblockingNF。KBactivationmaybeausefulstrategyincancertherapyThus，thisstudywasperformedtoexploretheeffectofparthenolide，anaturalNF—nBinhibito

4、ronhumanlungcancerA549cellstreatedwithlowdoseoxaliplatin，aswellastodeterminethepotentialmechanisminvolvedMethods：A549cellswereincubatedwitlldifferentconcentrationsofparthenolideintheabsenceorpresenceofalowdoseoxaliplatin

5、CellproliferationwasdeterminedbyMTTassayandflowcytometryWasusedtostudyapoptosisPGE2productioninculturesupernatantswasdetectedbycompetitiveELISA，whileexpressionofNFKB／p65，COX一2，caspase一3andcaspase一9proteinswereanalyzedbyW

6、esternblotResults：Comparedtoparthenolideoroxaliplatinalone，significantimprovementsincellapoptosisandgro、機hinhibitionindexeswereobservedinthecombinedtreatmentNFK：B／p65，COX一2，andPGE2expressionweresuppressedbytheCOapplicati