規(guī)?；i場大腸桿菌、沙門氏菌耐藥性檢測與分析.pdf

1、V939519工分類號密級弋啜寰弋夭禹’碩士學(xué)位論文單位代碼：10019學(xué)號：S030437規(guī)?；i場大腸桿菌、沙門氏菌耐藥性檢測與分析SurveillanceandAnalysisontheAntimicrobialResistanceofEscherichiacogandSalmonellafrom8nIntensivePigFarm研究生：指導(dǎo)教師：合作指導(dǎo)教師：申請學(xué)位門類級別：專業(yè)名稱：研究方向：所在學(xué)院：二00六年六月一一址

2、幽坳盟幽■，‘，雪鬣AbstractEscherichiacoil幔colOandSa／rⅢme／laageimportantzoonosispathogenstheantimierobialresistanoeofwhichisofgreatinterestinrecentyearsAntimicrobialresistanceandanalysisofmsistancerelatedgenesofEcoliandSalmonella

3、fromanintensivepigfarminBeijingwerecarriedoutonthebasisoftheiridentification136Ecelland62SalmonellawelgrecoveredfromfecalsampleafterbiochemicaleharacterizationAllisolateswereserogroupedwithOantiseraofEcoliandA～FofSalmone

4、lla76isolateswereplacedinto13differentserogroups，accountingfor559％ofthetotalEcoli，with0141，018，0107，020and0131beingthepredominantonestakingupto763％ofthetypableisolates19SalmonellaisolateswemdeterminedwithA—Fantisetamakin

5、gupt0306％oftheisolatesPCRWasemployedtodetectentemtoxingenesin136EcoliisolatesItshowedthattherewere38positiveOnes。accouiingfor279％ofallEcoliisolatesPositiveisolatesofst／andfromspringWel“epredominantlydetected，thepercentag

6、eofwhichis191％(26／136)and373％(28／75)respectivelyAntibioticresistanceto20differentdragswasdeterminedbymicrobmthdilutionmethodThemajorityWasresistanttomultipleclassesofantimicrobialsAllisolatesofEcoliwereresistanttomoretha

7、n4ofthe20antibiotics，625％wereresistanttomorethan10drags，withthemostseverecaseof18antibioticsresistedinasigleisolateaccoutingfor07％ofthetotalisolatesIsolatesofSalmonellawereresistanttomorethan2ofallantibiotics，613％wereres

8、istanttomorethan10drugs，withthemostseverecaseof18antibioticsresistedinasigleisolateaccoutingfor32％ofthetotalisolatesThefrequencyofresistancetooxytetrucycline，tetracycline，sulfadiazine，chlortetracyeline，doxytetraeyclinean

9、damoxlcillinwas990％，990％，98％，924％，894％and879％AllisolatesweresensitivetoceftriaxoneResistancetodinoxacin，sarafloxacin，eiprofloxacin，emofloxacin，oftoxacinandnorfloxacinwas692％，434％，379％，348％，328％and318％respectivelygyrA0RDR

10、of36EscherichiacoilandSalmonellaofdifferentquinoloneresistanceandclass1integronamong136Ecoliand62Salmone／lawereamplifiedbyPeRAnalysisofthesequencesrevealedthattherews$noalternationinaminoacidsinquinolonesensitiveones，one

11、mutationat83or87wasfoundinthelowlevelquionloneresistantisolatesandmutationsatbothpositionswasindicatedinl迦hlevel刪OfleresistantonesClasslintegonswe坤identifiedin42Ecoiland32Salmonellarespectivelyandnoquinoloneresistantgene