灰葡萄孢除草活性菌株的誘變及其相關(guān)基因的差異顯示.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文灰葡萄孢除草活性菌株的誘變及其相關(guān)基因的差異顯示姓名：馬娟申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物病理學(xué)指導(dǎo)教師：董金皋張金林20060613MutationandDifferentialDisplayofHerbicidalrelatedGenesofBotrytiscmemaAuthor：MAJuanSupervisors：DONGJingaoZHANGJin|nMajor：PlantPathologyAbstractI

2、twasreportedthatmycotoxinsfromBotrytiscinereaCallbedevelopedasmicrobialherbicidesduetotheirhighherbicidalbioaetivityHoweverthetoxigenichereditymechanismofBotrytiscinereaisnotclearatpresentT11efungi踟砂晰cinemaisaknownpathog

3、enoftomatograymouldInthisthesisherbicidalsubstanceandthegenesofBotrytiscinemathatregulatedtoxinproducingwerestudiedbyisolateBC4andjtsmutantisolateSThemainresultsareasfollows：(1)Treatedbyultravioletrayandchemicalmutagen，5

4、0isolateswereobtainedfromisolateBC4’SconidialsuspensionScreeningbybiometricsandHPLC(highlyeffectiveliquidphasechromatography)，threeisolates(B1，B2，B3)withhigherherbicidalactivityandtwoisolates(B5，B23)withlittleherbicidala

5、ctivitywereselectedout(2)HPLCconditionswerestudied，andtheresultsofHPLCanalysisshowedthattheherbicidalcontentinisolatesB1，B2，andB3weremuchhigherthanthatinthewildisolateTheirbiggestabsorptionpeakvalueisatleastfivetimeshigh

6、erthanthatinthewildisolateButthequantityofeachcomponentinstrainB5andB23iSobviouslydroppedcomparedwithwildisolateAtthesametimewefoundtheirherbieidalactivitydidnotchangeaftertheywerecultured8generationsOptimizedHPLCconditi

7、onsweredetermined，andfourfractionsfromthemetaboliteofthemutantisolateswereobtainedAmongthemthefractionIandⅣhadstrongherbicidalactivitytoDigitariasanguinalisandAmaranthUSretroflexus(3)Inordertofindoutthemolecularmechanism

8、ofherbicidalmetaboliteproducinginBotryt括cinema，mRNADDRTPCR(differentialdisplayreversetranscriptionPCR)wasusedtOanalysistheexpressedgenesofisolateBC4anditsmutantisolatesDifferentiaIdisplayedeDNAfragmentsthatrelatedtoherbi