拉莫三嗪對戊四氮慢性致癇大鼠海馬細胞凋亡的影響.pdf

1、蘭州大學碩士學位論文拉莫三嗪對戊四氮慢性致癇大鼠海馬細胞凋亡的影響姓名：雷軍申請學位級別：碩士專業(yè)：神經(jīng)病學指導教師：王為民20090501EffectoflamotrigineonapoptosisinhippocampusofratswithchronicallykindledepilepsybypentylenetetrazoleABSTRACTObjective：toexploretheantiapoptoticeffectof

2、lamotrigineontheexpressionofBcl一2andBaxinhippocampusofratswimepilepsyinducedchronicallybypentylenetetrazole(PTZ)Methods：EightyhealthyadultWistarratswererandomlydivivedbyweightintoeightgroupsincludingnormalgroup，modelgrou

3、p，lamotriginetreatmentgroups(Iamotriginelow，medium，highdosegroups)andvalproicaciddrugpositivegroups(valproicacidlowmedium，highdosegroups)10ineachgroupPTZ(35mg／kgd)wasinjectedintraperitoneallyinthemodelgroupandlamotrigine

4、treatmentgroupsandvalproicaciddrugpositivegroupseverydaytacinekindlingcriterion(about2weeks)tomakethechronicepilepticmodels，andthesamevolumephysiologicalsalinewasgiventothenormalgroupatthesamewayThenthesamedoseofPTZ(35mg

5、／kgd)wasinjectedonceeveryotherdaytomaintainkindlingmeanwhile，thediffenrentdosevalproicaciddrugpositivegroupsandlamotriginetreatmentgroupsweregastricallyperfusedwithcorrespondingdosageofvalproicacidandlamotrigineonceadayf

6、or3continuousweeksrespectivelyAndthesamevolumephysiologicalsalineWasgiventothenormalgroupandmodelgroupatthesamewayTheexpressionofBcl2andBaxinhippocampalneuralcellsofallgroupsweredetectedbyimmuno—histochemistymethodResult

7、s：Comparedwithmodelgroup，theexpressionofBcl一2andBaxWaslowerinnomalgroup；TheexpressionofBcl2washigher，andthatofBaxwaslowerindiffenrentdoselamotriginetreatmentgroupsandvalproicaciddrugpositivegroupsComparedlamotfiginetreat

8、mentgroupswithvalproicaciddrugpositivegroups：Therewerenodiffenrencesbetweenlow—dosegroups(BCL一2P=0139；BaxP=0346P005)，Thereweredifferencesbetweenmedium—dosegroups(Bcl2P=002BaxP=0044P005)，andthereweresignificantdiffenrence