pGRF基因質(zhì)粒對(duì)免疫應(yīng)激斷奶仔豬生長的影響.pdf

1、_v14ffsZ05分燕號(hào)蚯衛(wèi)墨墼墜塑墅壁盟四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文pGRF基因質(zhì)粒對(duì)免疫應(yīng)激斷奶仔豬生長的影響董海軍指導(dǎo)教師王廑士班宣量學(xué)科專業(yè)動(dòng)塑螢差皇縫整鞋堂研究方向坷抖獨(dú)田皇壘翅敦垃遷定四JIl雅安2007年12月EffectofpGRFGenePlasmidontheGrowthofImmunechallengedWeanedPigletsDongHaijunDirectedbyProfWangKang—ning(Animal

2、NutritionInstitute，SichuanAgriculturalUniversity，Yan’all，Sichuan，PRChina625014)AbstractThepurposeofthisstudywastoevaluatetheeffectofpGRFgeneplasmidontheperformanceandimmunologicalfunctionofimmune—challengedWeanedPigletsf

3、or35士2d，andalsothepotentialmechanismoftheseeffectswerediscussedEighteenweanedDLYpigswithageof35士2dandinitialbodyweight(B叨786a：059kgwerenotcastratedandrandomlyassignedtothreetreatmentsaccordingtogenderandBWbysinglefactord

4、esignThethreetreatmentswerepGRFgeneplasmidgroup，pGRFgeneplasmidpluslipop01)rsaccharide(LPS)groupandLPSgroupEachtreatmenthadsixreplicatesandonepigletineachreplicateDietarytreatmentdidnothaveadditiveantibioticsDuringthethr

5、eeweeks’experimentthepigletswereallowedadlibitumtofeedandwaterFeedconsumptionaveragedailygain(ADG)，feedtogainratioif／G)anddiarrhea丘equencywereinvestigatedOnd1oftheexperiment，pGRFgeneplasmidandpGRFgeneplasmidplusLPSgroups

6、wereonlyonceinjected1OrngpGRFgeneplasmidperheadandonlyonetime，andLPSgroupwereiIljcotednormalsalineOnd8and15，pGRFgeneplasmidplusLPSandLPSgroupswereinjcotedLPSaccordingto100pgperkLlogrambodyweight，andpGRFgeneplasmidgroupwe

7、reinjectednormalsalineOn2hafterLPSchallenge，bloodsampleswerecollected，Intheperipheralbloodserum，thelevelsofIgGweredeterminedusingimmuno—turbidimetrymethod，andIGF—I，ILlandIL6wasdeterminedbyELISAtechniqueTheresultswereasfo

8、llows：1ThepigletsinthepGRFgeneplasmidplusLPSgrouphadabeaergrowthperformancethanthoseintheLPSgroup(PO05)，andworsethanthoseinthepGRFgeneplasmidgroup(P001)TheADGofpigletsinthepGRFgeneplasmid，pGRFgeneplasmidplusLPSandLPSgrou