影響小鼠核移植胚發(fā)育因素的研究.pdf

1、湖南農(nóng)業(yè)大學碩士學位論文影響小鼠核移植胚發(fā)育因素的研究姓名：李垚申請學位級別：碩士專業(yè)：動物遺傳育種與繁殖指導教師：薛立群20050601StudyoflnfluenceFactoronNuclearTransferEmbryosDevelopmentofMiceAbstractAgreatdealoffactorshasmultipleeffectsontheSUCCESSrateofcellnucleartransferBeside

2、stheeffectofmaintainingploidyofreconstructedovumcoreandreprogrammingthedonornuclei，treatmentmethodofoocyte、micro—operatingtechnology、differentcytoplastrecipientanddifferentdonornucleianditsstatehaveimportantinfluenceonco

3、nstructdevelopmentofembryoTherecipientisoocytesinmetaphaseII，donornucleiisthegranulecellinthisexperimentInfluencefactorsonreconstructedembryoinnucleartransfertechnologyofmicewerereserched，Theresultsasfollows1、Thecumulus—

4、enclosedoocytecomplexestreatedwithhyaluronidase，theconcentrationandtreatedtimebothinfluencethedevelopmentofreconstructembryo、斫thmoveovumtubeblowingandbeatingfor2minrepeatedlycallobtainbetterreconstructembryodevelopmentra

5、te(808％、2、ThisexperimentcomparedifferentparameterofelectricityactivateandageinfluenceontheoocyteactivateOocytestreatedwithsamepulsestrength，pulsedurationpulsetimes，Theoocytesat17hcangethighactivatingrate(717％1anddevelopm

6、entrate(848％1394％、Inthesameage，pulsedurationandpulsetimes，Theoocyteactivateratereachedtipto“727％)whenthepulsestrengthatthe100V／mm，2cellandblastuladeveloperatealseachedpeakvalue(85o％／35o％)，SOthispulsestrengthwassuitablefo

7、roocytesactivationInthesameage，pulsestrengthandpulsetimesPulsedurationatthe809s，theoocyteactivateratereachedfiptop(767％)，2cell，blastuladeveloperatebothreachpeakvalue(804％／348％)，SOthispulsedurationwassuitableforoocytesact