黑龍江銀鯽與普通鯽的遺傳多態(tài)的比較研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文黑龍江銀鯽與普通鯽的遺傳多態(tài)的比較研究姓名：李因傳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：嚴(yán)云勤2001.5.1碩十學(xué)位論文摘要2001年5月ComparativeinvestigationofthegeneticdiversitybetweenC.a.gibelioandC.a.auratusAbstractThesilvercruciancarp(CarassiusauratusgibelioBlocht

2、riploidy)fromnorthernwellknownforitsgynogenesisanditsbisexualpopulation.Surprisinglyitmighthavegonochoristicreproductionwhenexperimentalproofforit.Owinginseminatedbyhomologoussperm.Buttheretoitsidenticalmoroholo2vwithCar

3、assiuChinaisaswellstillhasauratusratus(diploidy)anditsspecificgeneticbackgroundthegeneticrelationshipbetweennoautriploidanddiploidfromthesameplacesmaybethekeytoinvestigatetheoriginofCarassiusauratusgibelioBlochanditsspec

4、ificreproductivemode.BycomparingseverallevelsofpolymorphismbetweenwildlydiploidindividualsandwildtriploidiesamplifiedbyprimersfromCDSsequencesgenesequencesandmicrosatellitelociwefoundtherewerenodifferencebetweenC.a.gibel

5、ioandCarassiusauratusauratusinlowpolymorphicloci.Inmoderatelydiverselocisuchassomemicrosatellitesthewildtriploidiesandthewilddiploidiessharedthesamebandpatternsorgenotypeswhileinhighlypolymorphicmicrosatellitelocibothsha

6、redthevastmajorityoftheDNAfragmentsandsomeofthebandpatterns.TheindividualsofthetriploidandthediploidoverlappedinthephylogenetictreeandformedmanysmallclustersbytheUPGMAanalysisofSSLPbandpatterns.Fromthebandpatternsandthei

7、rphylogenetictreeweknowthatthetriploidhaveasmuchdiversityasthediploidwhichshowedC.a.gibeliodidntatallappeartobethetypicalcharacteristicsofpureclones.Thesinglestrandconformationalpolymorphism(SSCP)ofmtDNADloopregionshowed

8、thesamephenomenonbetweenthetriploidandthediploidtoo.Theveryspecialpolymorphicinformationhelpedusconstructthereproductiverelationshipmodelandevolutionarymodelbetweenandamongthetriploidanddiploid.ThemodelshowedC.a.gibelioB

9、lochoriginatedfromthediploidincertainenvironmentsuchascoldandevolvedtheabilityofanoxiatoleranceinicecoveredwaterthroughintraspecificpolyploidization.Thetriploidmightevolvefromtheextantdiploidincertaincircumstancesorhaveg

10、eneexchangeswiththediploid.Oncethetriploidformedtwokindsofreproductivemodesexistedinthem:gynogenesisandgonochoristicreproduction.Itsthegonochoristicreproductionthatdirectlyresultedinthemaletriploidindividualsandclonaldiv