13889.東鄉(xiāng)野生稻內(nèi)生放線菌fro2鑒定、培養(yǎng)條件優(yōu)化及活性產(chǎn)物分離

1、獨(dú)創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。據(jù)我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得或其他教育機(jī)構(gòu)的學(xué)位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示謝意。學(xué)位論文作者簽名：簽字日期：年月日學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解江西師范大學(xué)研究生院有關(guān)保留、使用學(xué)位論文的規(guī)定，有

2、權(quán)保留并向國家有關(guān)部門或機(jī)構(gòu)送交論文的電子版和紙質(zhì)版，允許論文被查閱和借閱。本人授權(quán)江西師范大學(xué)研究生院可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。(保密的學(xué)位論文在解密后適用本授權(quán)書)學(xué)位論文作者簽名：導(dǎo)師簽名：簽字日期：年月日簽字日期：年月日Identification，CultureConditionOptimizationandBioactiveProductSep

3、arationofActinomyceteFR02fromDongxiangwildriceAbstractEndophyteisofaiTmityandplantgrowthpromotingabilitytothehostplant，moreover，itsrichmetabolitesCanbeusedforbiologicalcontr01Sofar，about75％oftheantibioticswereproducedbya

4、ctinomycetesDongxiangwildrice(Oryzarufipogon)isanimportantgermplasmresource，anditsuniqueandrichendophyteisavaluablebiologicalresourcesEndophyticactinomycetesscreenedfromDongxiangwildrice(Oryzarufipogon)Canbeusedasthethea

5、griculturalantibioticdevelopmentofnewresourcesFouractinomycetes(FRol，F(xiàn)R02，F(xiàn)R03andtheFRhl)fromDongxiangwildriceweresubjectedtopreliminarytaxonomy，physiologicalandbiochemicaltests，andidentificationincludingmorphologicaland

6、culturecharacteristicsandphylogeneticanalysisbasedon16SrDNAgenesequenceanalysisFRol、FRhl、FR02andFR03wereidentifiedasStreptomycesvenezuelae，Streptomyceshygroscopicus，StreptomycesrocheiandStreptomycescarrlosusrespectivelyn

7、efermentationbrothoffouractinomyceteshasawideantibacterialspectrum，andstrainFR02isofantagonisticactivityforthemainlysevenplantpathogenicfungianddisplayspotentialofdevelopmentprospects111ephysiologicalandbiochemicaltestsr

8、esultsofstrainFR02showedthatoptimumgrowthtemperatureandpHis300CandpH8respectivelyItcouldgrowinISP2liquidmediumcontainingNaClconcentrationvariedfrom0to12％(w／v)StrainFR02couldhydrolysisstarch，gelatinliquefaction，Milkpepton

9、izationbutnotsolidification，Nitratenotdeoxidation，Cellulosehydrolysis，nomelaninproductionandnoH2SformationItcouldusedDxylose、Dfructose、rhanmose、DarabinoseandDglucosebutnotraffinoseItschemotaxonomicpropertiesfurtherdemons

10、tratedthatthestainsFR02isStreptomycesrochei111eantimicrobialsubstancehadgoodstabilityandinhibitoryactivitiesundertheconditionof501000C，pH210andUVirradiationInaddition，itcouldn’tbedegradedbyproteasesnlefermentationmediuma

11、ndfermentationconditionsofthestrainFR02wereoptimizedbySuppressionofmycelialgrowthresultsTheoptimalmediumcomposition：cornstarch4％，Soybean1％，starch2％，K2HP040025％，CaC0303％，KN030025％andNaCl005％。Theoptimalculturalconditionswe