四君子湯對北京鴨健脾助消化機理的研究.pdf

1、Y774865分類號：密級：單位代碼：10019學(xué)號：S02329‘j：嚼差遺夭普’學(xué)位論文四君子湯對北京鴨健脾助消化機理的研究StudyontheMechanismofFourGentlemenDecoctionImprovingDigestiveFunctioninBeijingDuck研究指導(dǎo)教師：迕劍莖熬援合作指導(dǎo)教師：穆搓熬援申請學(xué)位門類級別：盛堂亟專業(yè)名稱：蝤速獸醫(yī)堂研究方向：生耍整醫(yī)結(jié)金所在學(xué)院：動物匡堂瞳二零零五年五月A

2、bstractFourGentlemendecoctioniSextensivelyusedinclinicalpracticeforthetreatmentofSpleendeficiencypattern(SDP)However，onlyalittlebitwasknownaboutthemechanismofactionStudieshaveshownthatabnormalityofexocrlnepancreaticsecre

3、tionWaSamajorpathologicalmechanismofSDPCholecystokinin(CCK)andvasoaetiveintestinalpeptide(vIP)wasverypotenttostimulateexocrinepancreaticsecretionTheaimofthepresentstudyistoinvestigatetheeffectofFourGentlemendecoctiononth

4、eexpressionofCCKandVIPinduodenumandjejunumofduckSDPmodelsusingsemiquantitatiVereversetranscriptionpolymerasechainreaction(RTPCR)I，PrimersweredesignedaccordingtothesequencesofchickenCCKVIPandduckB—actincDNApublishedintheG

5、enBankTheexpectedsizesofDNAfragmentswereamplifiedusingRTPCRtechnique，thePCRproductswereclonedintopGEMTEasyvectorforsequenceanalysis，theresultsshowedthatthehomologiesofsequenceswere90％94％and100％respectively2ThemodelsofSDP

6、wereestablishedbyinjectingReserpine，expressionofCCK，VIPwasdeterminedbysemiquantitativeRTPCRTheexpressionlevelsofCCK，VIPmRNAinexperimentalSDPweresignificantlyreducedthanthatincontrols(P0，01)ThissuggeststhatCCKVIPplayakeyr

7、oleinSDP3NosignificantdifferencesintheexpressionlevelsofCCKVIPmRNAwerefoundinduodenumandjejunumbetweenFourGentlemendecoctionpreventivegroupandcontrolgroupTheexpressionlevelsofCCK，VIPmRNAinduodenumofSDPgroupweresignifican

8、tlylowerthanthatofpreventivegroup(P(O01)TheexpressionlevelsofCCK，VIPmRNAinjejunumofSDPgroupweresignificantlylowerthanthatofpreventivegroup(P005)TheseresultsindicatethatFourGentlemendecoctionhadpreventiveactiononSDP4Theex

9、pressionlevelsofCCK，VIPmRNAinduodenumandjejunumofspontaneousrecoverygroupweresignificantlylowerthanthatofcontrolgroup(P001)TherewerenosignificantdifferencesintheexpressionlevelsofCCK，VIPmRNAbetweengroupI，11treatedbyFourG

10、entlemendecoctionandcontrolgroupInduodenum，theexpressionlevelsofCCK，VIPmRNAingroupI，11treatedbyFourGentlemendecoctionweresignificantlyhigherthanthatofspontaneousrecoverygroup妒O01)，injejunum，thatisP005Nosignificantdiffere

11、nceswerefoundbetweengroupIandgroupIIThesefindingssuggestthatFourGentlemendecoctionmayexerttheirbeneficialtherapeuticactiononSDP，atleastinpart，byupregulatedCCK，VIPmRNAlevelsinduodenumandjejunumKeywords：FourGentlemendecoct