植物激活蛋白對(duì)煙草花葉病毒（TMV）的生物活性及作用機(jī)理初探.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文植物激活蛋白對(duì)煙草花葉病毒（TMV）的生物活性及作用機(jī)理初探姓名：陳梅申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：植物病理學(xué)指導(dǎo)教師：曹克強(qiáng)邱德文20050617StudyontheInhibitionEffectofPlantActivatorProteintoTobaccoMosaicVirusandtheMechanismInvolvedCandidate：ChenMeiSpecialty：PlaatpathologyAdvi

2、sor：PmfCaoKe—qiangProfOiuDe—wenAbstraetPlantvirosisiSoneofimportantdiseasesofcropsTheeffectivenessandsafetyfordiseasecontroliSfarfromdemandingofagriculturalproduction，SOitiSstillveryimperativetodevelopnewtyDeofantivirala

3、gents，PlantactivatoriSakindofthermostableproteinabstractedfromfungi，whichcarlpromoteplantgrowth，activatetheguardsystemofplantandinduceresistance，withtheadvantagesofbroadspectrum，innoxiousandenvironment—friendlyPreliminar

4、yresearchshowedthattheplantactivatorproteincouldcontrolplantviraldiseasesefficientlyInthisresearch，thecontrolefficiencyofplantactivatorproteininthesystemhost(NtabacumCVSamsum)andlesionhostfⅣtabacumCVXanthi—NC)ofTMVwaseva

5、luatedbyconventionalbiologicalandmolecularbiologicalmethods，suchasindirectELISA，WesternBlottingandsemiquantitativePCRTheresultsshowedthatplantactivatorproteincouldreachupto67，27％fortheprotectiveeffectanduptO6673％fortheth

6、erapeuticeffectonsystemhosts；ondetachedleaves，lesioncontrolrateofplantactivatorproteinreached4023％anditcouldinhibitlesions’extensionwithinhibitionrateashighas3939％IntobaccoplantstreatedwithplantactivatorproteinTMVcoatpro

7、teininlesionsdecreasedby2275％，inleafdiminishedby25％，andthecontentofRNAofTMVreducedby28％Theexperimentsdemonstratedthatplantactivatorproteincouldinducedefencesubstancesintobaccoseed】inganditsinductioneffectwassimilartOTMVA

8、tiertreatedwithplantactivatorprotein，theactivitiesofSOD，POD，PALaincreasedintobaccolcavesAmongwhich，theactivityofSODreachedpeakonthe4thdayaftertreatment，increased155timescomparedwithcontrolgroup；andtheactivitiesofPODPALre

9、achedpeak013the13thdayafterplantactivatorproteintreatmentrespectively，respectivelywhichwere2timesand178timesofuntreatedcheckTakingActingenethatiswidelyhomeoticandhighlyconservedineukaryoteastheinternalcontrol、theexpressi

10、ondifferenceofPR一5∞gene—aantivirusrelatedgeneamongⅣtabacumCVXanthiNCleaveswithdifferenttreatmentswasmensuratedbysemi—quantitativeRT—PCRmethodItwasfoundthattheexpressionofPR—jc，Bgenereachedpeakonthe5thdayafterplantactivat