燒灼大鼠表層鞏膜靜脈法誘導建立慢性青光眼模型.pdf

1、青島大學碩士學位論文燒灼大鼠表層鞏膜靜脈法誘導建立慢性青光眼模型姓名：（艸問）雪靜申請學位級別：碩士專業(yè)：眼科學指導教師：王竫華2003.3.5論文英文摘要TheEstablishmentoftheRatModelofChronicGlaucomabyCauterizingEpiscleralVessels【Abstract】ObjectiveToprovideareproducible，quantitative，easilymadea

2、ndlowcostglaucomamodelwithfewcomp]icationsinWistarratsMethodsThreeepiscleralvesselsofWistarratswereunilaterallycauterized，whilethelateraleyeskeptuntouchedforcomparisonTheratintraoeularpressurewasmeasuredandrecordedregula

3、rlyRateyeswereenucleatedafter2months，4monthsand6monthstoobservethechangesoftheopticdiskandtheretina，tomeasurethehorizontaldiameterofthecorneaandtocountthenumberofretinalganglioncellsincentralretinaandinperipheralretinain

4、bothexperimentalandcontralateraleyesThethicknessofcentralandperipheralretinawasalsomeasuredandcomparedResults①Thediameterofratcorneadidnotchange②ElevatedIOPpersistedforatleast6monthswithoutre—treatment，andanapproximately

5、18foldincreaseinlOPafter6months。comparedtothecontralateraleye35016vs1941ImmHg，respectively)③Therewasanapproximately46％lossofretinalganglioneellsintheperipheralretina，and36％lossinthecentralretina④Cuppingoftheopticdiskwaso

6、bservablebyexamination，andC／Dwasover06after6months⑤ThethicknessofretinabecamethinnerConclusion①ThemodelofchronicglaucomacouldbeestablishedbyunilaterallycauterizingtheepiscleralvesselsofWistarrats⑦Thethicknessofretina，the

7、numberofretinalganglioneellsandthecupoftheopticdiskwerechangedsignificantly③Thismodelprovidesareproducibleandquantitativemodelforpharmacologicalexperiments，damageofopticnerve，neuroprotectionandtreatmentoftheopticneuropat