人小細胞肺癌細胞株H446側(cè)群細胞的生物學(xué)特征.pdf

1、蘇州大學(xué)碩士學(xué)位論文人小細胞肺癌細胞株H446側(cè)群細胞的生物學(xué)特征姓名：王波申請學(xué)位級別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：張軍寧20100501AbstractBiologicalcharacteristicsofthesidepopulationofhurnansmallcelllungcanc蟹竺墮』墊e旦!塹Biologicalcharacteristicsofthesidepopulationofhumansmallcelllungc

2、ancercelllineH446AbstractObjective：Theaimofthisstudyistoidentifyandcharacterizethesidepopulation(SP)cellsinsmallcelllungcancer(SCLC)celllineH446，whichlaythefoundationforisolationandpurificationofCSCMethods：Fluorescenceac

3、tivatedcellsorting(FACS)wereusedtosortSPandnonSP(NSP)cellsfromH446BothsubgroupswerecultivatedtosurveythecapabilityofreformingintosuspendedtumorcellspheresReversetranscriptionPCR(RTPCR)andrealtimePCRwereusedtoevaluatethem

4、RNAexpressionlevelsofCD133，ABCG2andNucleostemininbothsubgroupsThecapacityofproliferationandthedifferencesindrugresistanceofbothsubgroupsandunsortedcellswastestedbyMTTmethodThedifferentiationabilityofbothsubgroupswasdeter

5、minedbyFACSTheproliferationofthemwasdeterminedbysubcutaneoustumorformationinnudemiceResults：(1)ThepercentageofHoechst33342negativecellswasabout51402％inH446byfluorescencemicroscopyThepercentageofSPcellswas6301％byflowcytom

6、etry(2)SPcellshasstrongercapabilityofreformingintotumorspheresthanNSPcells(3)ThemRNAexpressionlevelsofABCG2，CD133andnucleostemininSPcellswere2160a：026，710x014and102_L008foldhigherthanthoseinNSPcells(P005)(4)Invivo，SPcell