實(shí)時(shí)熒光定量PCR檢測臨床標(biāo)本中淋病奈瑟菌.pdf

1、青島大學(xué)碩士學(xué)位論文實(shí)時(shí)熒光定量PCR檢測臨床標(biāo)本中淋病奈瑟菌姓名：趙立紅申請學(xué)位級(jí)別：碩士專業(yè)：病原生物學(xué)指導(dǎo)教師：于紅2002.9.22一薹塞塑星AbstractDetectionofNeisseriaBmorrhoeeebytheCulLureandtheRealLimFIuoresmmoeQuantitativePolymraseChainR鰍i硼ObjectjveTodetecttheDNAofNeisseriagonorrh

2、oeaeinciinicalgenitourinaryspecimensbythereal—timefluorescencequantitatirePolymeraseChainReaction(FQ_PCR)。andtoprovideareliablesupportfortheclinicaldiagnosisandtreatmentofgonerrhoeaMethodsIn173clinicalgenitourinaryspecim

3、ens，Neisseriagonorrhoeaes’DNAweredetectedbyNeisseriagonorrhoeaereal—timefluorescenceI)cRdiagnostickitwithPE5700automaticfluorescencePCRanalysisinstrument，Atthesametime，thespecimenswereisolatedbyTMmediumwithPolyVitex，iden

4、tifiedbyATBExpressionandthematchedAPINHAgentsusceptibilitytestwasdeterminedbyATBExpressionandthematchedATBNHThe昌一lactamaseoftheNeisseriagonorrhoeaesweredetectedbyaciditypapermethodResuIts0f173clinicalspecimens，withreal—t

5、imefluorescencequantitativePCR，42caseswerepositiveandthepositiveratiowas243％，amongthem，23casesweremaleurethrasecretions，18werefemalewombsecretions，1wasprestatefluid；withthegermiculturemethod，41caseswerepositiveandtheposi

6、tiveratioWaS237％amongthem。22casesweremaleurethrasecretions，18werefemalewombsecretions1WaSprostatefluidWhencomparedtothegermiculture，thespecifityofFQPCRwes100％，sensitivityWaS976％theagreementwas994％ConeIusionIndetectingNei

7、sseriagonorrhoeaes，thesensitivityofFo～PCRishigherthanthatofgermiculture，F(xiàn)Q—PcRisasimple，rapid，andspecificmethodtoanalyzeNeisseriagenorrhoeaes，itisofpotentialuseforclinicaldiagnosisButatthesametimeofgermiculture，theagents