玉米轉(zhuǎn)錄因子ABP2在轉(zhuǎn)基因水稻中的抗逆功能分析.pdf

1、貴州大學(xué)碩士學(xué)位論文玉米轉(zhuǎn)錄因子ABP2在轉(zhuǎn)基因水稻中的抗逆功能分析姓名：孫宏申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：國興明趙軍20070301AbstractAbiotics燈es％，suchasdroughthi咖salinityandlowtemperaturecanmakeplantsdehydratedwhicheventuallyleadstorapidincreaseofreactiveoxygen印eci髂

2、(Ros)ROShavedualeffectsOnplants；ontheoneband。aM娜ROScauseoxidatives蹶swhichresultsinirreversible畫IIryatbothmolecularandcellularlevel；ontheotherhandasasignalingmolecule。ROSparticipateinABAmediatedresponsestostresses，includi

3、ngenhancingtheexpressionofanfioxidantgenesDissectingthemolecularmechanismunderlyingtheregulationofantioxidantgenesisofgreatin平優(yōu)切n∞inimprovingthepe面ormanceofpl卸臨understressesCatalase(CAT)isthemainsinkofH202inplantsandplay

4、sfillindispensableroleinshessdefenceTranscriptionfactorABP2whichspecificallyinteractswithallABAresponsiveelementofmaizecatlhasbeenclonedinourlaboratoryandpreviousstudieshavedemonstratedthatABP2cmactivatetheexpressionofre

5、portergeneinbothyeastcellsandmaizesuspensioncellsTheresearchexpressthatABP2啪improvostresstoleranceinArabtdopsisTheresultsobtainthroughthepresentresearcha∞asfollows：1Werecivedtransgenticrice62341928—1and1928—13byPCRmethod

6、2TheexpressionofABP2wemanalyzedbyNorthernTheresultsshowedthatABP29eneWasdifferentexpressedintheselines3ThesurvivalresultsexpressthattransgenticABP2ricecanimprovedroughttoleranceandsalttoleranceunderdrought、saltd1％4Physio