FISH與IHC用于50例浸潤性乳腺癌HER2檢測的相關(guān)性分析.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文FISH與IHC用于50例浸潤性乳腺癌HER2檢測的相關(guān)性分析姓名：符鼎申請學(xué)位級別：碩士專業(yè)：普通外科學(xué)指導(dǎo)教師：郭建昇肖虹20090421CorrelationofFISHandIHCfordetectingHER2statusin50casesofinvasivebreastcancerAbstractObjective：Toinvestigatethecorrelationbetweentheresult

2、softhetwomethodsFISHandIHCusedtotestHER2statusfor50casesofinvasivebreastcancenMethods：50casesofinvasivebreastcancerwerecollectedrandomlyfromthefirsthospitalofShanxiMedicalUniversityandallofthemhadacceptedoperationfrom200

3、8to2009WeutilizedFISHandIHCrespectivelyforprocessingconsecutiveCUtsheetofeachcasetotestHER2status，andthananalysisedthecorrelationbetweentheresultsoftwomethodsResults：1Seventeencases(34％)ofHER2geneamplificationweredetecte

4、din50easesofbreastcancerbyFISHFifteencasesshowedpositiveexpression(3、2～3、2)basedonIHC，and35caseswerenegative(1、0)；2、ItisprovedthatheresultsofFISHandIHChavecorrelationbyChi—SquareTests(X2=2324，P0001)：3、RegardingFISHastheg

5、oldstandardwecangetthatthesensitivityofIHCis765％，specificityis939％，accuracyis880％，Youden’Sindexis0704，positivepredictivevalueis867％andnegativepredictivevalueis886％。Conclusion：SincetheresultsofFISHandIHChaveexcellentcorre

6、lation，wecanregardIHCasscreeningtechniquetodetectHER2statusforthepatientswithbreastcancertodeterminwhetherHerceptinischoosedWesuggestthatthepatientswithbreastcancerprovedtobepositive(3、2～3、2)basedonIHCshouldchooseFISHtod