北極狐親子鑒定及產(chǎn)仔數(shù)相關基因的研究.pdf

1、東北農(nóng)業(yè)大學碩士學位論文北極狐親子鑒定及產(chǎn)仔數(shù)相關基因的研究姓名：宗文麗申請學位級別：碩士專業(yè)：特種經(jīng)濟動物飼養(yǎng)指導教師：白秀娟20070620StudiesofRelatedMoleculeMarkeronLitterSizeandParentageTestinArcticFoxAbstractThearcticfoxwaftaseasonaloestrusanimalItonlybredonceinayearDuringaoestr

2、umthefemalefoxeswhichwemraisedinthefarmswerefertilizedwith3malefoxes’spermIftheirparentagecouldnotbeidentified，thearcticfoxeswouldbeinbreedingNotonlythesearcticfoxesbutalsotheirfltrwerebadbecauseofinbreedingAndthetinpotf

3、urwouldhavenocompetencetobesoldintheworldmarketInordertoavoidinginbreesingitwasveryimportanttomakingatruefamilytreebytestparentageLitterofsizewasaimportanteconomiccharacterinthearcticfoxefarmIfLittersizeofarcticfoxesWaSi

4、ncreased，thearcticfoxes’furwouldbeincreasedAndeconomiceffectwouldbegainedItwasoneofimportantaimstoincreaSelittersizeoffoxeseffectivelyforthefoxbreedingBecausefittersizeofarcticfoxwasalowhefitabflityquantitativecharacteri

5、twasdifficulttoincreaSelittersizeofarcticfoxbygeneralinbreedingtechnologyButthesedaysitwaslikelytosettletheproblembymodemmoleculebiologictechnologyInordertofindouttheproblemparentageofagroupoffoxesWaStestedthroughmircosa

6、tellitemarkerandsomecandidategenesonlittersizeoffoxwerestudiedthroughPCRSSCP皿eresultswereasfoHows●(DinthetestparentagewastestedbyPC冉SSRsuccessfullyin73samplesfortllefirsttimeAndC皿was9999％(2)Forthefirsttimepartofestrogenr

7、eceptor餌SRkeneexonlsequencewereclonedwithonesoffollicle—stimulatinghormoneBsubunit(ram)geneexon3sequenceandfolliclestimulatinghormonereceptor(FSHR)geneexonl0sequenceinthearcticfoxinthetest(3)AllelefrequencyofAWaSmorethan

8、oneofBinESRgeneinarcticfoxesAndgenotypesfrequenciesofAAwasmorethanooesofABandBBforESRgeneinarcticfoxesAllelefrequencyofAwasmorethanoneofBforFSHggeneinarcticfoxesAndgenotypesfrequenciesofAAwasmorethanonesofABandBBforFSFIB

9、geneinarcticfoxesAllelefrequencyofCwasmorethanoneofDforFSHBgeneinarcticfoxesAndgcnotypesfrequenciesofCCwasmorethanonesofCDandDDforFSHBgcneinarcticfoxesGenotypesfrequenciesforESRgeneandFSHl3geneaccordedwithHardy—Weinbergl

10、aws(4)SignificantassociationswerefoundforFSHBgnewithlitterofsizeinarcticfoxesOnemutationlocusWaSfoundinESRexonlThedifferentgenotypesofESRhadasignificantlydifferenttrendeffecton1ittersizeofarcticfox(P=O0591)Twomutationloc