陜西與湖北松屬植物上散斑殼屬的分類和該屬雙基因系統(tǒng)發(fā)育學(xué)研究.pdf

1、 II Abstract Taxonomy of Lophodermium on pines in Shannxi and Hubei provinces and the genus’s phylogenetics based on two­gene sequences were studied by modern fungal taxonomic method, and 7 species of Lophodermium

2、on pines were found, respectively Leptostroma magnum, Leptostroma coalescens, Lophodermium pinastri, Lophodermium kumaunicum, Lophodermium nitens, Lophodermium cedrinum and Lophodermium himalayense. Among them Lepto

3、stroma magnum and Leptostroma coalescens were new anamorphic fungi which always attach to Lophodermium while their teleomorphs existing. All type specimens were deposited in the Reference Collection of Forest Fungi of An

4、hui Agricultural University, China (AAUF). The research content mainly includes the following two aspects. Firstly, relatively systematic taxonomy of Lophodermium on pines was mainly studied using the traditional taxonom

5、ic method. Two new species were preliminarily established by external examination and internal microexamination of frozen section, detail description of morphological features, internal structure characteristics and so o

6、n in both English and Chinese were given, and the point line composite image of conidiogenous cells, conidiophores and trichogynes were given. At the same time, the authors also investigated and studied in geographic dis

7、tribution, ecological habitat of these species. Secondly, combining the molecular biology and bioinformatics methods, based on rDNA­ITS, 28S rDNA sequence and two­gene sequences, the phylogenetic trees were con

8、structed to explore the interspecies and intraspecies relationships of the Lophodermium members from Shannxi and Hubei provinces. Total DNA were extracted by CTAB method, the correlation sequences were amplified by PCR t

9、est, blasted them in GenBank and selected Leotia lubrica Fr. of Helotiales as outgroup, then phylogenetic trees were constructed respectively to identify the species classification status combining with morphological cla

10、ssification method. The results showed that the same species of different strains usually preferred to cluster together, or different species with the same region and host are also likely to gather together. No matter th

11、e phylogenetic trees based on rDNA­ITS, 28S rDNA sequences or two­gene sequences, all strains of Lophodermium on pines were gathered in Clade Ⅰ, and strains of Lophodermium on hardwood generally gathered in Cla