長角血蜱雌蜱唾液腺蛋白質(zhì)及激素調(diào)控的研究.pdf

1、河北師范大學(xué)博士學(xué)位論文長角血蜱雌蜱唾液腺蛋白質(zhì)及激素調(diào)控的研究姓名：李海龍申請學(xué)位級別：博士專業(yè)：動物學(xué)指導(dǎo)教師：劉敬澤20050520博1=學(xué)位論文：長角血蜱雌蜱暉液腺啦白質(zhì)及激索調(diào)控的研究ACNAPSBSACCDcDNACHAPSDIGE縮略語acetonitrileammoniumpersulphatebovineserumalbuminchargecoupleddevicecomplementaryDNA乙腈過硫酸銨牛血清白蛋

2、白電荷耦臺裝置互補(bǔ)DNA3一【(3cholamidopropyl)dimetllylammonjo]13[(3膽酰胺丙基)二乙胺]propanesulphonatedifferentialin—gelelectrophoresis2D—PAGEtwo—dimensionalpolyacrylamidegeleDTTEDTAreslsdithiotllreitoethylenediaminetetraaceticacidESlMSMSel

3、ectrosprayionizationtandemESTExPASyGCGCMSHPLCIAAICATIEFIPGLC—ESIFTICRMS丙磺酸膠內(nèi)著示電泳ectropho一雙向聚丙烯酰胺凝膠電泳二硫蘇糖醇乙二胺四乙酸massspectro一電噴霧電離串聯(lián)質(zhì)譜merryexpressedsequencetag表達(dá)序列標(biāo)簽expertproteinanalysissystem蚩自質(zhì)分析專家系統(tǒng)gaschromatography氣相色譜

4、gaschromatography—massspectrometry氣相色譜質(zhì)譜聯(lián)f；f儀highperformanceliquidchromatography高效液相色譜iodoacetamide碘乙酰胺isotopecodedaffinitytagging同位素編碼親和標(biāo)簽isoelectricfocusingimmobilizedpHgradient等電聚焦固相pH梯度iquidchromatographyelectrospray