銀杏內(nèi)酯類物質(zhì)產(chǎn)生菌的選育及發(fā)酵條件初步研究.pdf

1、成都中醫(yī)藥大學(xué)碩士學(xué)位論文銀杏內(nèi)酯類物質(zhì)產(chǎn)生菌的選育及發(fā)酵條件初步研究姓名：羅靜申請學(xué)位級(jí)別：碩士專業(yè)：生藥學(xué)指導(dǎo)教師：嚴(yán)鑄云2007-05成都中醫(yī)藥大學(xué) 2004 級(jí)碩士學(xué)位論文 iiAbstract Ginkgolides are a kind of terpene lactones with great medical potential in central-nerve system and blood-brain barrie

2、r system,et al.It’s also the most effective natural antagonist of platelet activating factor.Because ginkgolides are unique components of Ginkgo biloba L,which are reported to possess peculiar structure and remarkable ph

3、armacological activities.The experiment ties to solve the problem of sustainable utilization of ginkgolides resource through using the feature that endophyte can produce some metabolites similar to those from the host pl

4、ant and microbial fermentation. The experiment use the method of TLC and HPLC to screen out a high ginkgolides producing strain FG 052 from active endophytic fungi which were isolated from Ginkgo biloba L, morphologicall

5、y identified as Aspergillus fumigatus var. fumigatus.By taking FG 052 as starting strain, a higher productive mutant strain m4-4 was obtained after UV mutation and compound mutation of UV and nitrous acid.The productivit

6、y reached 181 mg/L,which was 56% more than that of the parent strain Aspergillus fumigatus var. fumigatus FG 052.The mutant’s genetic stability was good after five generations. The results of experimenting on optimum fer

7、mentation conditions of strain FG 052 showed that PDA is the optimum heredity culture medium for stain FG 052 growth and producing spores and Czapek is the optimum fermentation culture medium,the maximum amount of myceli

8、a appeared at fermenting for 168 h,the maximum ginkgolides productivity appeared at 144 h and yield reached 0.13 mg/mL,pH is 4.86 here.Ginkgolides content in each flask is the highest when C/N is 10.Influence of C source

9、,N source,pH on the ginkgolides production were studied systematically.Optimum medium components for ginkgolides production are as following :N source is NaNO3(0.3 %), C source is glucose ( 3 % ),pH is 7.0.The optimum se

10、ed age was 18 h,the optimum liquid culture volume was 30 mL in 250 mL Erlenmeyer flask,the optimum inoculum amount was 1% of the liquid culture volume,and the optimum temperature was 26 . ℃ The experimental research ab

11、ove lay a important foundation in studying the new resource of ginkgolide and industrialization production in future. Key words ： ginkgolides, endophytic fungi, screening and identification, breeding, fermentation optimi