2023年全國(guó)碩士研究生考試考研英語(yǔ)一試題真題（含答案詳解+作文范文）_第1頁(yè)
已閱讀1頁(yè)，還剩15頁(yè)未讀， 繼續(xù)免費(fèi)閱讀

下載本文檔

版權(quán)說(shuō)明：本文檔由用戶提供并上傳，收益歸屬內(nèi)容提供方，若內(nèi)容存在侵權(quán)，請(qǐng)進(jìn)行舉報(bào)或認(rèn)領(lǐng)

文檔簡(jiǎn)介

1、TheeffectofearlyauditydeprivationontheagedependentexpressionpatternofNR2BmRNAinrataudityctexCaixiaBiYileiCuiYutingMaoJinghongXuFengXuJipingZhangXindeSunResearchCentrefBrainScienceEastChinaNmalUniversityShanghai200062Chin

2、aAbstractUsinginsituhybridization(ISH)presentresearchminutelyinvestigatedtheagedependentexpressionpatternofNR2BmRNAinrataudityctex(AC)duringpostnataldevelopmenttheeffectofearlyauditydeprivation.Ftheratsinthenmalgroupthem

3、RNAexpressionofNR2Bwashighestatbirth(postnatalday1)butdeclinedrapidlyuntillowlevelofadulthoodduringwhichtherewasarisingperiodwithatransientpeakonpostnatalday21(P21)ftheratsinthedeprivedgroupthewholedecliningasagingtrendo

4、fNR2BmRNAexpressionwassimilartothatofnmalgroupbuttheexpressionlevelfromP15toP27weremuchlowerthanthatofnmalgroupwiththegreatestreductionfromP21toP23thetransientpeakonP21disappeared.InbothgroupsthedistributionpatternofNR2B

5、mRNApositiveneuronsin6layersdsalmedialventralsubdistrictsofaudityctexwasalsoexamined.Thereisnosignificanteffectontheexpressionpatternbetweennmaldeprivedgroup.Ourresultsindicatedthattheearlyauditydeprivationdecreasedtheag

6、edependentexpressionpatternofNR2BmRNAsuggestingthatNR2Bmayplayanimptantroleinthedevelopmentalplasticityofaudityfunctioninrats.Keywds:AudityCtexAudityDeprivationInsituhybridization(ISH)NR2BmRNAExpression1.IntroductionDuri

7、ngasensitiveperiodinpostnataldaysanimalsshowafmofexperiencedependentdevelopmentplasticityincentralnervoussystem.IthasbeenproposedthattheNmethylDasparate(NMDA)receptplayacrucialroleduringthiscriticalperiod.Withagethechang

8、esofNMDARfunctionmayberelatedtothevarietyofthesubunitcompositionwhichincludetheNR2BNR2A[3040].IntheneoctextheNMDAreceptiscomposedofNR1NR2subunits.NR2ANR2BthetwoNR2subunitshaveattractedfocusesrespectively.Someresearchprop

9、osedthatNR2BmightpromoteplasticitymethandoestheNR2Asubunit[414344].NakanishihavedemonstratedthatmRNAencodingNR2Bwashighlyexpressedthroughoutthefebrainwithespeciallyhighlevelsintheolfactybulbshippocampusctex.Levelswereund

10、etectableintheponsmedullacerebellum[32].In﹡Crespondingauth.XindeSunEmail:.severalareasofcerebellumbrainstemtheexpressionleveldecreasedrapidlyin7daysafter:www.paper.11.3InsituhybridizationInsituhybridizationwasusedwiththe

11、signdetectionreagentboxesboughtfromtheBoehringerCompany.TheDiglabeledoligonucleotideprobefNR2BmRNAwassynthesizedbyShanghaiBioengineeringCompanywiththesequencesasfollows:5GGGCCTCCTGGCTCTCTGCCATCGGCTAGGCACCTGTTGTAAACC3ddUT

12、PDig.Atleasttwoanimalsofeachdetectionagewereusedfinsituhybridization.SDratsweredeeplyanaesthetizedwithinjectedPentobabital(50mgkg.BW)perfusedwith0.9%Naclthen4%Parafmaldehyde(PFA)in0.1MPhosphatebufferedsaline(PBS).Thebrai

13、nthatobtainedafterdecapitationwasimmediatelyfixedin4%PFAPBSf2hoursthenwasimmersedinto20%30%SucrosePBSrespectivelyfdehydrationtillthebrainpedonthebottom.Subsequentlyconarysectionsof30mwerecutbyafreezingmicrotomethawmounte

14、donto1%glutincoatedslidesthendriedin40Cdesiccationboxovernight.Brainsectionswerepretreatedwith0.1MPBSGlyPBSTritonPBSf10minutesatroomtemperature(RT)respectivelythenincubatedintoproteinaseK(1gml)TEbufferf30minutesat37C4%PF

15、Af5minat4C.Slideswerethenincubatedwith170lprehybridizationsolution[47%FmaeDeionized2salinesodiumcitrated(SSC)0.05Mphosphatebuffer(PB)]f2hoursat37C.Afterawashin2SSCf5minutesslideswerecoveredwith170lhybridizationsolution[4

16、7%FmaeDeionized2SSC0.05MPB1Denhard’ssolution10%dextransulfate(DS)0.05MDithiothreitol(DTT)0.25mgmlyeasttRNA0.5mgmldenaturalizedsalmonspermicDNA2gmlDiglabledoligonucleotideprobefNR2BmRNA]f18hoursat37C.Followingarewashesof2

17、SSC1SSC0.25SSCrespectivelyf2timesof15minutesat37CBufferⅠf2timesof10minutesatRTtreatingofBlockingbufferf30minutesatRTincubationwithantibodysolutionf2hoursat37CwashesofBufferⅠBufferⅡf2timesof10minutesrespectively.Thenslide

18、swerecoveredby170lNBTBCIPsolutionf18hoursindarkboxatRT.BufferⅢwasusedtostopthecolationreactionthenbrainsectionswererestainedbyfastgreenf2minutesfollowedbywashesofwaterf2timesof10minutesdehydrationofdescendingethanolthefi

19、nalsealing.Thecontrolexperimentsftheprobe’sNR2Bspecificitywereperfmedthesamewayexceptthattheprobewasexcludedfromthehybridizationsolution.TheexaminationcountingofpositiveneuronsinACwasperfmedthroughopticalmicroscopesusing

20、thepositiveneuronsdensityasanindexfcomparison.TheratACcontains6layersthatcanbedividedintoventralmedialdsalareas(Fig.1.a)accdingtotherelevantdatareference[15].Atleast20consecutiveslicesinthesamebrainareaofeachanimalweresu

21、rveyed.Weexaminedtheaudityctexunderhighpowermicroscope.16gridsweresetas1mm2thepositivecellsineachgridwascountedthenthedensityofpositiveneuronsdensitycanbeobtained(Nmm2).ThedatawasexpressedasMeanSEwiththestatisticalsignif

22、icancedeterminedbyttest.2.Results2.1TheexpressionofNR2BmRNAinaudityctexToinvestigatetheNR2BexpressionweanalyzedthemRNAleveleverydayfrompostnatal1to21thenevery3daysafterP18.Fig.2showesthedramaticdecreaseofNR2BmRNAinACof:w

溫馨提示

  • 1. 本站所有資源如無(wú)特殊說(shuō)明，都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請(qǐng)下載最新的WinRAR軟件解壓。
  • 2. 本站的文檔不包含任何第三方提供的附件圖紙等，如果需要附件，請(qǐng)聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
  • 3. 本站RAR壓縮包中若帶圖紙，網(wǎng)頁(yè)內(nèi)容里面會(huì)有圖紙預(yù)覽，若沒(méi)有圖紙預(yù)覽就沒(méi)有圖紙。
  • 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
  • 5. 眾賞文庫(kù)僅提供信息存儲(chǔ)空間，僅對(duì)用戶上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理，對(duì)用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯，并不能對(duì)任何下載內(nèi)容負(fù)責(zé)。
  • 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容，請(qǐng)與我們聯(lián)系，我們立即糾正。
  • 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時(shí)也不承擔(dān)用戶因使用這些下載資源對(duì)自己和他人造成任何形式的傷害或損失。

最新文檔

評(píng)論

0/150

提交評(píng)論