36620.殼聚糖固定化蚯蚓纖溶酶研究

1、河北大學(xué)碩士學(xué)位論文殼聚糖固定化蚯蚓纖溶酶研究姓名：韓麗梅申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：武金霞20080601AbstractAbstractE積hwonn舶rinolyticenz)，lTleswereinlInobilizedonchitosan謝tllglutaraldehydebyadsorbandcross—linkreactionTheoptimalconditionSforiI啪obilizatio

2、nwere嬲follows：1g出tosallwithpH65phoSphatebu疏rsolutionwaStreatedwitll5mLl％百utaraldehydesol戚onat25℃for8h，a＆rrinsingnlec柚rosaIlthoroll出ly’13Uear她Ⅳ0nn舶rinol舛cen2秒messolution、Ⅳereaddedinto鋤dadsorbedonthecarrierat4℃for6h，afterr

3、msiIlgtheuninⅡIlobilizedenzymes、Ⅳithlmo兒NaCl，therec0Ve巧ofenz)，meactiVitywasabout63％alldthespeci萎bactiVit)rofirrⅡnobilizedenzyn托swaSO082U^：ngTheoptimaltemperatureforfreeandil姍obilizede啕呻eswere50℃，舭irop岫a1pHwere85and8，0re5

4、忱ctivelythethem∞stabili夠鋤dtllepHstabili夠ofilnmobilizede玎馮豫eswe聆enhallcedcomparedwiththatof舶ee啕，mesThee蚴，IIlescouldhy出0lyze舶血ogenaIldfiHna‰rbeingimmobilized，howeVer，theimmobiIizedenzymescouldIlothydrolyzeBSAanymoreBo血tIle

5、盤eeem畔s雅d也e灑1mobilized豇圮ymescou撼nothydfolyzeSuperoxideDismutaSe，HemeglobinaIld11lrombinogenComponent8w鶴immobiIizedonc11itosan謝mglutaraIdehydebyadsorbaIldcross—linkreactio瑪Theoptimalconditionsfor油mobilizationwereaSfOllOws

6、：lg幽tosanw‰pH70phosphatebufrersolutionwaStreated、析th5mL1％glutaraIdehydes01utionat25℃for4kmen30|工LComponent8waSaddedinto以er血singthec11itosannlorougllly趾Idadsorbedonthecarrierat4℃fIor6KaRerrinsingtlleu11immobilizeden巧mes誦t

7、h1mo兒NaCl，therecoVe巧ofenzymeactiV時(shí)wasabout453％，aIldtllespecifictheactiV毋ofimmobilized朋zymew2LsO0086U／|ng。Botllt11e五哈eComponent8and也eimmobilizedConlponent8couldhydrolyze邱咖ogen，butcouldnothydrolyzeBSAanymoreKeywordsearthwo