l.plantum和e.coli細胞壁肽聚糖緩釋微球?qū)π∈竽c道菌群及機體免疫調(diào)節(jié)作用的研究

1、分類號一UDC一密級署哦瀘產(chǎn)篝碩士研究生學(xué)位論文Lplantum和E口D／／細胞壁肽聚糖緩釋微球?qū)π∈竽c道菌群及機體免疫調(diào)節(jié)作用的研究申請人：范佳惠學(xué)號：2121231培養(yǎng)單位：生命科學(xué)學(xué)院學(xué)科專業(yè)：微生物學(xué)研究方向：微生物產(chǎn)品開發(fā)與制備指導(dǎo)教師：孫慶申完成日期：2014年3月27日黑龍江大學(xué)碩士學(xué)位論文AbstractThebiological’activitiesofpeptidoglycanaregettingmoreandmor

2、eattentionThisthesisaimstocomparetheeffectsofmicroencapsulatedpeptidoglycanfromgrampositivebacteria(representedbyLplantum)andgramnegativebacteria(representedbyEcoli)onintestinalfloraandsystematicsimmunitywherebyproviding

3、experimentaldataforpotentialvaccinedevelopmentofpeptidoglycanEcolipeptidoglycanwasseparatedbytrichloroaceticacidmethod，with(112135)％extractionyieldEcoliprptidoglycan(EPG)microsphereswaspreparedbychemicalcrosslinkingproce

4、ssusingchitosanaswallmaterialsScanningelectronicmicroscopy(SEM)resultsshowedgoodspherityEPGmicrospheresThismicrosphereshaslaodingcapacyof(4351466)g／1009andencapsulationrateat(9120182)％Theresultsfromregulationeffectsofpep

5、tidoglycanmicrosphereonmouseintestinalfloraandimmunefuctionshowedthatthenumberofprobioticflorasuchaslactobacillusandbifidobacteriumintheintestinaltractsfrommousetreatedwithpeptidoglycanmicrospheresissignificantlyhigherth

6、anthosefromsaline。treatedgroup(P001)；andconditionalpathogenicbacteriasuchasenterobacterandenterococcusweresignificantlylowerthanthoseinnormalsalinegroup(POo1)Contentsofserumlysozyme，IgGandSIgA，liverandspleenindexesweresi

7、gnificantlyhigherinpeptidoglycanmicrospheres—treatedgroupthanthoseinnormalsalinegroup(P001)，TheprotectioneffectsofpeptidoglycanmicrospheresagainsttheEcoliinfectedmouseshowedthatthenumberofhealthy—promotingbacteriainthein

8、testinaltractsfrompeptidoglycanmicrospheretreatedmousewassignificantlyhigherthanthoseinnormalsalinetratedgroup(P0O1)；thenumberofconditionalpathogenicbacteriawassignificantlylowerthanthoseinnormalsaline—tratedgroup(POO1)S