嚴(yán)重?zé)齻聠蝹€(gè)核細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)機(jī)制變化的實(shí)驗(yàn)研究

1、第二軍醫(yī)大學(xué)博士學(xué)位論文嚴(yán)重?zé)齻聠蝹€(gè)核細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)機(jī)制變化的實(shí)驗(yàn)研究姓名：于寶軍申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：燒傷外科指導(dǎo)教師：夏照帆葛繩德2001.5.1童三呈墾盔蘭————一一一墮墮塑壅蔓圭堂焦絲壅PBMCSIRSCARSMARSMODSMoFRTPCREMSA縮略詞表Peripheralbloodmononuclearcells外周血單個(gè)核細(xì)胞SystemicInflammmatoryResponseSyndrome全身性炎癥反應(yīng)綜

2、合癥CompensatoryAntiintlammatoryResponseSyndrome代償性抗炎癥反應(yīng)綜合征MixedAntagonisticResponseSyndrome失代償性炎癥反應(yīng)綜合征Multipleorgansdysfunctionsyndrome多臟器功能不全綜合癥Multipleorgansfailure多器官功能衰竭Reversetrascriptionpolymerasechainreaction逆轉(zhuǎn)錄PCR

3、ElectrophoreticMobilityShiftAssaysDNA蛋白結(jié)合凝膠電泳位移分析Thl／Th2cytokilieThelper1／2cytokineNACN—acetylL—cysteineNF—KBnuclearfactorkappaBTACtotalantioxidantcapacityIL一1，2～13intedeukin12～13FAKfocaladhesinkinaseIrd3一d，pinhibitorypr

4、otein，cB儀，BTNF—oL，ptumornecrosisfactor娃，BIFNyimerferon吖NOni廿iCoxideOFRsoxygenfreeradicalsTBSAtotalboaysurfaceareaPBSphosphatebuffersalineBSbumserumSODsuperoxidedismutaseCATcatalaseAP一1NOSH202O，。OHROCCICRactivatingprotein

5、111itricoxidesynthasehydrogenperoxidesuperoxideanionrcdicalhydroxylradicalreceptor—operatedC礦channelCa2一inducedCa“releaSeT輔助細(xì)胞1或2型細(xì)胞因子N一乙酰L型半胱氨酸核因子一KB總抗氧化能力白細(xì)胞介素一1，213粘著斑激酶抑制蛋白KB噸，B腫瘤壞死因子戧，8Y干擾素一氧化氮氧自由基體表總面積磷酸鹽緩沖液燒傷血漿超氧化