轉(zhuǎn)基因抗蟲棉品種DNA指紋圖譜建立和遺傳多樣性評價.pdf

1、河北農(nóng)業(yè)大學碩士學位論文轉(zhuǎn)基因抗蟲棉品種DNA指紋圖譜建立和遺傳多樣性評價姓名：潘玉欣申請學位級別：碩士專業(yè)：作物育種學指導教師：馬峙英20050616EstablishmentDNAFingerprintingandEvaluationGeneticDiversityofTransgenicInsectresistantcottonAuthor：PanYuxinMajor：CropGeneticsandBreedingSupervis

2、or：ProfMaZhiyingAbstractInthispaper52transgenicinsect—resistantcottonvarietiesofGossypiumhirsutumfromChinaandabroadIhavebeenusedtoinvestigatefiberqualitytraits，constructclusterdengrogramBasedontheSSRandAFLPanalysis，weana

3、lysisedthediversityandconstructedthefingerprintingofthecultivarsThemainresultswereasfollows：1TheanalysisofthefiberqualitytraitsshowedthatthereweredifferenceamongtheexperimentmaterialsTheresultsshowedthatthetransgenicinse

4、ct—resistantcottoncan’tmeettheneedsoftextileindustryenoughThefiberlengthvarietiesrangedfrom28mmto30mmthefiberstrengthWaSwithin23O～316cNtex一，themicronaireWasfrom4O～5。6andthelintpercentagewasfrom3。6～4。4Thevarianceoffiberst

5、rengthwasthebiggestamongthetraitsamongthecoefficientsofvariance213polymophicprimercombinationswereselectedtoperformtheSSRandAFLPfingerprinting32cultivarshavespecificbandsaccountedfor615％Everyprimercombinationwasdifferent

6、intheidentificationefficiencyofcoltivarsNoprimercombinationcoulddistinguishallthecultivars3TheanalysisbasedthefiberqualitytraitsshowedthattheEuclideandistanceWasamong056～792andtheaverageEuclideandistancewas342Thepairwise

7、Euclideandistanceofthevarietiesrangedfrom252to556Itshowedthatlittledifferenceexistedamongdifferenttestedvarieties4TheEuclideandistancewasamong200～557andtheaverageEuclideandistancewas421bySSRandAFLPThepairwiseEuclideandis

8、tanceofthevarietiesrangedfrom373to475whichshowedthatthegeneticbasicamongthetestedvarietiesisnotabundance5Withthemorphologicalcharacters，thematerialsallcouldbedividedintothreegroupsWiththetWOmolecular，thematerialsallcould

9、bedividedintothreegroupstooIneverygroup，thereweresomeeultivarsfromdifferentbreedingprovincesThecultivarsineverygroupwerenotconsistentintwomethods6Sixvarieties(1ines)werescreenedform52testedmaterialsbasedonanalysisofmorph