幽門螺桿菌卵黃抗體的研制.pdf

1、Y940303分莢號s8524學4j200i520517南京愛黃愛蓬碩士學位論文幽門螺桿菌卵黃抗體的研制謝獻勝指導教帥～土錳一蠱一堰一一工諺～—型!：究旦一研究領(lǐng)域～一養(yǎng)殖一研究方Ⅲ一～一動物生主一一答辯日期一一三Q9魚鈕L丑一PREPARATIONOFYOLKANT狂；ODYAGAINSTHELICOBACTERPYLORIDifferentculturemediaweretestedtocultureandpreserveHelic

2、obacterPyloriflip)ThejustmaturechickenswereprocedurallyimmunizedwithcompleteHpwhichculturedbytheteam，agglutinationtestwereusedtodeterminetheeggyolkantibodylevel，andthepatternofimmuno—responsion，bacteriostasisinVitro，phys

3、icalandchemicalcharacteristicofimmuneyolkweresearchedAllthesestudieswereofgreatsignificancetotheindustrialproductionofhighimmuneyolkantibodyagainstUpandcorrelativelybiologicmedication1EffectofdifferentmethodsonHpculturea

4、ndpreservationThestrainwasculturedonColumbiaAgarBase(CAB)supplementedwithmixedantibioticscontaining2500IU／1PolymyxinBSulfate，5mg／lTrimethoprim，10mg／1VancomycinHCland10mg／1AmphotericinBand6％defibrinatedsheepbloodor6％horse

5、serumrespectivelyinamicroaerophilicatmosphereof5％02，10％C02and85％N2at37。Cfor72hWiththetwomethods，goodeffectwasobservedInconditionofsterilization州t11nosupplementation誦t11mixedantibiotics，thestrainCan’tbecontaminatedandgrow

6、wellaswellWhenBrucellaBrothWasusedanculturemediumwithcombinedantibioticsandhorseseruminmicroaerophilicbag，UpcouldgrowrapidlybutWaseasilytobespheroidwithoutreplacingthebagontimeAndtheliquidculturemethodwascontaminatedcomp

7、ared晰thsolidmethodThestrainCanberevivedsuccessivelywitllcryopreservationat一70。Cto80。C，100percents，2PreparationandBacteriostasisinVitroofEggY0lkAntibodyAgainstHelicobacter&loriPreparationandbacteriostasisinvitroofeggyolka

8、ntibodyagainstHelicobacterpylori(Hp)havebeenstudiedChickenswereprocedumllyimmunized謝tllcompletebacterinat150，165and180daysold，successivelyAgglutinationtesthasshownthattheeggyolkantibodyagainstHPWasproducedfromimmunizedhe