圓錐角膜的超微結(jié)構(gòu)觀察及細(xì)胞凋亡的表達(dá).pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文圓錐角膜的超微結(jié)構(gòu)觀察及細(xì)胞凋亡的表達(dá)姓名：韓玉萍申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：眼科學(xué)指導(dǎo)教師：賈亞丁李冰20060518山西醫(yī)科大學(xué)碩士學(xué)位論文UltrastructureStudyofKeratoconusandtheEvidenceofApoptoticCellDeathAbstractPURPOSE：Toexplorespecificpathologicalchangesinkeratoconusbyobser

2、vingtheultrastructureofkeratoconuswithlightmicroscopyandtransmissionelectronmicroscopytesttheevidenceofapoptosisineachlayerofkeratoconusandnormalcomea，thusdiscoverthemechanismsofkeratoconusMETHODS：5normalhumancomeasand11

3、keratoconusCOlTleaswerecutintoparaffinsectionandultrathinsectionbyroutinemethods，weobservedtheirchangesofeverylayerbylightandelectronmicroscopyParaffinsectiontissueswereexamined11istopathologicallyforTUNELimmunoreactivit

4、ytodetectthepresenceofDNAfragmentationRESULTS：Thefollowingchangeswerefoundunderlightmicroscopyinkeratoconus：thinepitheliumandstromalocalruptureordestructioninbasementmembranesandBowman’Slayerbasalcellswollen，stromadisarr

5、angement，rupturesorfoldsintheDescemet’SmembraneandthinendotheliumlayerComparedwithnormalcorneaundertransmissionelectronmicroscopykeratocyteofkeratoconushadsmallernucleuswithlesshetetochromatin，moreandlargermitochondria，i

6、ncreasedandenlargedroughendoplasmicreticulmandgoigicomplexofirregularstructure，theapoptosisbodyWasfoundinsomecasesandlargeamountofgranulardepositsamonglaminae3normalcorneasexhibitedfewerthanfiveTUNEL—positiveepithelialce

7、llswhichwereverylightlystainedIncontrast，weobservedthatmoreandheavystainedTUNEL—positivecellscouldbefoundin10keratoconuscomeasfatleastonelayer)among1leasesThepositivestainingcellswereseenintheepitheliumlayerof10cases，int

8、hestromallayerof8casesandintheendotheliumlayerof6cases6casesdemonstratedTUNELstaininginallthreelayersCONCLUSION：Keratoconusisanoninflammatoydegenerativedisease，wesuggestapoptosisasflmodeofcelldeathbyobservingitsultrastru