孕激素在淋球菌引起的中性粒細胞癥反應中的調節(jié)作用.pdf

1、華中科技大學碩士學位論文孕激素在淋球菌引起的中性粒細胞癥反應中的調節(jié)作用姓名：帥俊申請學位級別：碩士專業(yè)：皮膚病與性病學指導教師：涂亞庭200905013EffectsEffectsEffectsEffectsofofofofprogesteroneprogesteroneprogesteroneprogesteroneononononPMNPMNPMNPMNinflammatyinflammatyinflammatyinflammat

2、yresponsesresponsesresponsesresponsestotototogonococcusgonococcusgonococcusgonococcusDepartmentofDermatologyvenereologyUnionhospitalTongjimedicalcollegeHuazhongUniversityofScienceTechnologyMasterdegreeecidate:ShuaiJunSup

3、ervis:Prof.TuYatingAbstractAbstractAbstractAbstractObjectiveObjectiveObjectiveObjectiveToevaluatetheeffectsofprogesteroneonPMNinflammatyresponsestogonococcustodiscusshowitinfluencesasymptomaticgonococcalinfectionofwoman.

4、MethodsMethodsMethodsMethods①Collecttheexudatesfromurethracervixofgonrheapatients.N.gonrhoeaewereculturedonThayerMartinagarGCbasedchocolateagaridentifiedbyGramstainoxidasereaction..ThentheyweresuspendedinPBSadjustedtoanO

5、D600=0.15(~108gonococciml1).②Neutrophilsisolatedfromheparinizedhumanperipheralbloodwereromlydividedintofourgroups:progesteronegroup(A)gonrhoeaegroup(B)progesteroneinterventiongroup(C)controlgroup(D).③Theexpressionlevelso

6、finflammatyfactsiNOS，TNFαIL1βmRNAinneutrophilswereassessedbyrealtimeRTPCRineachgroupatvarioustimepoints(0381224h).④iNOSproteinlevelsfgroupBgroupCweredetectedbyWesternblot.ResultsResultsResultsResults①RealtimeRTPCRindicat

7、esthattheexpressionlevelsofiNOS，TNFα，IL1βmRNAincreasedingroupBCreachedtheirpeakat8hoursingroupB.GroupChadanobviouslowerexpressionthanB(P0.05).TherewerenosignificantchangesingroupAD.②WesternblotshowedthatiNOSproteinalsoha