結(jié)直腸癌患者外周血CD4+T細(xì)胞亞群的表達(dá)及臨床意義.pdf

1、劉國(guó)芳結(jié)直腸癌患者外周血CD4T細(xì)胞亞群的表達(dá)及其及臨床意義一1一結(jié)直腸癌患者外周血CD4T細(xì)胞亞群的表達(dá)及臨床意義中文摘要【目的】檢測(cè)結(jié)直腸癌(colorectalcarcinoma，CRC)患者外周血Thl、Thl7、Th22和Treg細(xì)胞的表達(dá)變化，初步分析探討Thl、Thl7、Th22和Treg細(xì)胞與CRC臨床分期的關(guān)系以及在CRC發(fā)病機(jī)制中的可能作用?！痉椒ā扛鶕?jù)CRCDukes分期將33例CRC患者分為兩組：早期CRC組(n

2、=17)和進(jìn)展期CRC組(n16)；所有入選病例均未接受過(guò)放化療或其他干預(yù)性治療。收集33例CRC患者及25例健康志愿者(正常對(duì)照組)外周血標(biāo)本，運(yùn)用流式細(xì)胞術(shù)(Fluorescence—activatedcellsorting。FACS)分析CRC患者外周血中Thl細(xì)胞、Thl7細(xì)胞、Th22細(xì)胞及Treg細(xì)胞／CD4T細(xì)胞的比例；探討Thl細(xì)胞、Thl7細(xì)胞、Th22細(xì)胞及Treg細(xì)胞與CRC患者臨床分期的相關(guān)性。探討CRC巾是否存

3、在Treg和Thl7的平衡及Thl7／Treg與CRC病理發(fā)展的關(guān)系?！窘Y(jié)果】1)CRC組外周血中Thl細(xì)胞／CD4T細(xì)胞的比例與健康對(duì)照組相比差異無(wú)統(tǒng)計(jì)學(xué)意義(p005)；早期CRC組外周血中Th!細(xì)胞／CD4T細(xì)胞的比例與進(jìn)展期CRC組比差異無(wú)統(tǒng)計(jì)學(xué)意義(pO05)；CRC組外周血中Thl7細(xì)胞／CD4T細(xì)胞的比例明顯高于健康對(duì)照組(p005)：早期CRC組與進(jìn)展期CRC組外周血中Thl7細(xì)胞／CD4T細(xì)胞的比例明顯高于健康對(duì)照組(

4、pO05)；進(jìn)展期CRC組外周血中Th22細(xì)胞／CD4T細(xì)胞的比例明顯高于健康對(duì)照組比(p005)；進(jìn)展期CRC組外周血中Thl7／Treg的比值低劉國(guó)芳結(jié)直腸癌患者外周血CD4T細(xì)胞亞群的表達(dá)及其及臨床意義3英文摘要ExpressionofCD4十TcellsubsetsincolorectalcarcinomaandtheirclinicalsignificanceABSTRACT[Objective]Toinvestigateth

5、eproportionofperipheralbloodThl，Thl7，Th22andTregcellstothepatientswithCRC，preliminaryanalyzethecorrelationofTill，Thl7，Th22andrregcellswiththeclinicaltypingofCRC，andtheprobablerolesofThl，Thl7，Th22andTregcellsinthepathogen

6、esisofCRC【Methods1PatientswithCRC(n=331weredividedintotwogroups：thosewithearlydisease(n=17)correspondingtoDukesAaccordingtotheDukesclassificationforCRCandthosewithadvanceddiseasecorrespondingtoDukesB，CandD(n=16)Noneofthe

7、patient：；receivedradiotherapychemotherapy，orothermedicalinterventionsduringthisstudyThirty—threeadultCRCpatientswereenrolledinthisstudyandtwenty—fivehealthysubjectsweretakenascontr01Usingfluorescence—activatedcellsorting

8、wedeterminedthelevelsofThl，Thl7，T1122andTregcellsintheperipheralbloodof33CRCpatientsand25healthydonors，andevaluatedtheircorrelationswithtumorstageThepotentialrelationshipbetweenThl7andTregcellsintheperipheralbloodandthei

9、rfunctionsinthedevelopmentofCRCwereinvestigated[Results]1)Comparedwithhealthydonors，thepercentagesofThl7cells，Th22cells，andTregcellswereinceasedinpatientswithCRC(p005)Thepercentagesofpatientsintheearlygroupwereslightlyhi

10、gherthanthatintheadvanceddiseasegroup，butthisdifferencehasnostatisticalsignificance(p005)；PatientswithadvancedandearlydiseasehadanobviouslyhigherpercentageofThl7cellsthanhealthlycontrols(∥001)；Comparedwithearlydisease，th

11、eproF，ortionofperipheralbloodTh22cellsinCRCpatientswithadvanceddiseasehasnos：gnificantdifference(p005)；PatientsintheadvanceddiseasegrouphadanobviouslyhigherpercentageofTh22cellsthanhealthlycontrols(p001)2)Patientswithadv