腦腫瘤干細(xì)胞放射敏感性的實驗研究.pdf

1、東南大學(xué)碩士學(xué)位論文腦腫瘤干細(xì)胞放射敏感性的實驗研究姓名：柯金申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)（腫瘤）指導(dǎo)教師：何杰20090501一‘●●l●●J●●JExoerimentalresearch0重radl0SenSltlVllVforbraintumorstemcellKeJinHeJieAbstractObjective：Toevaluatedifferenceofradiosensitivityforbraintumorstemce

2、ll(BTSC)andhumangliomacelllineSHG44，toinvestigatetheexpressionofBTSCingliomatissues，andtoestimatetherelationbetweenBTSCandgliomabiologicalcharacterMethods：SHG44wasculturedinDMEM佰l2supplementedwith10％FBS，andinserumfreeDME

3、M／F12containingbFGFandEGFrespectivelyBTSCWasdetectedbyimmunocytochemistricalstaining、撕ⅡlantibodiesagainstCD133BTSCandSHG44wereirradiatedbyXrays誠mdosesof005，1，2，3，4，6，8，10，20Gyrespectively，andflowcytometryWascarriedouttod

4、eterminetheapoptosisandanalyzethechangesofcellcyclesClonogerticsurvivalcurvesofBTSCandSHG44weredeterminedbythemeasureofcellsurvivalfractionTheexpressionofCD133in45gliomatissuesWasdetectedbyimmunohistochemistricalstaining

5、andtherelationbetweentheexpressionandgliomamalignancyWasanalyzedTheexpressionofp53，MGMTandKi67proliferationindexWasassessedintheCDl33positivegliomagroupandtheCDl33一negativegliomagroupResultsOSHG44containedBTSC，whichcould

6、surviveandgrowbymeansofsuspensioninserum—freemediumandproliferateintotheclonalsphereTheclonalspheresmaintainedstrongabilityofselfrenewalandproliferationThespecificmarkerCD133couldbeexpressedbyBTSCAfterBTSCandSHG—44wereir

7、radiatedbyX—rays，flowcytometryassayshowedthatnodifferencecouldbefoundincellcyclesandthattheratiosofapoptosisinBTSCwerelowerthanthatinSHG44significantly(P005)TheClonogenicsurvivalcurvesassayshowedthatcellcloneformationinB

8、TSCWashigherthanthatinSHG_44(P001)CDl33couldbedetectedingliomatissues，andthereWasasignificantdifferencebetweentheexpressionofCD133inhighmalignantgliomasandthatinlowmalignantgliomas儼O05)Thedifferencesofp53andMGMTexpressio