生長激素、生長抑素對人胰腺癌細(xì)胞增殖作用的實(shí)驗(yàn)研究.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文生長激素、生長抑素對人胰腺癌細(xì)胞增殖作用的實(shí)驗(yàn)研究姓名：丁銳申請學(xué)位級別：碩士專業(yè)：外科學(xué)普通外科指導(dǎo)教師：倪泉興2001.5.1生堡整壅：生量塑塞翌△墮墮堡塑墮墮墮堡旦塑塞竺塹窒——————羔塑望!墨一EffectofHumanGrowthHormoneandSomatostatinonHumanPancreaticAdenocarcinomaGrowthBackgroudTheroleofhumangrowthh

2、ormone(hGH)asanutritionaladjunctforcancerpatientsiscontroversialbecauseofitspotentialmitogeniceffectsontumorgrowthTheenhanceproliferationeffectofhGHonhumanleukemicblastshasbeenreported，butfewstudieshaveexaminedthee腑ctofr

3、GHonpancreaticadenocarcinomaSomatostatininhibitsproliferationofmanysolidtumorsuchaspancreaticadenocarcinomaThepurposeofthisstudyistoexaminetheeffectofgrowthhormoneandsomatostatinonpancreaticadenocarcinomacelllinePC3invit

4、roandinvivoMethodsInvitro：PC3cellswereseededinto96一wellplatesatadensityofl103／WellAfter24hrofincubation，thecellculturemediawasremovedandserunlfreemediawithhGHorsomatostatin(1ng3“∥m1)wasaddedGrowthofPC一3cellswasexaminedby

5、MTTassayInvivo：twentyfiveAthymicmicewereinoculated(sC)withhumanpancreaticcarcinomacellsOnweek4twentymicewererandomizedto4groupsteceivedailyhGH(GH，n=5)，ortwicedailysomatostatin(SS，n=5)，orbothofthem(GHSS，n=5)，orsaline(CTL，

6、n=5)，subcutaneouslyTumorsizeweremeasuredandmicewereweightedeveryweekOnweek8tumorswereexcisedandcellcyclekineticsanalyzedDataareexpressedasmeanSDStatisticalanalysiswasperformedbyAnalysisofVarianceResultsInvitro：theprolife