大豆提取物提高小鼠免疫功能及抑制裸鼠的人肝癌原位移植瘤.pdf

1、北京協(xié)和醫(yī)學(xué)院中國醫(yī)學(xué)科學(xué)院碩士學(xué)位論文大豆提取物提高小鼠免疫功能及抑制裸鼠的人肝癌原位移植瘤姓名：郭靜申請學(xué)位級別：碩士專業(yè)：生理學(xué)指導(dǎo)教師：許榮焜20090501中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院碩上學(xué)位畢業(yè)論文EffectsofSBEonImmuneFunctionoflmmunosuppressiveMiceandHumanOrthotopicHepatocellularCarcinomatransplantedinnudemiceA

2、bstractTheinfluenceofsoybeanextract(SBE)011immunefunctionofimmunosuppressivemicewasinvestigatedinpresentstudyTheinhibitoryeffectsofSBEonhumanorthotopictransplantedhepatocellularcarcinoma(HCC)ofnudemiceandthemechanismsofr

3、egulatingthecellcyclewerealsoinvestigatedInpartI，immunosuppressivemiceWasestablishedbyintraperitonealinjectionofcyclophosphamide(CTX)toinvestigatetheeffectofSBEonimmunefunctionofimmunosuppressivemice。功edataindicatedthats

4、pleenindexandIgG2alevelweresignificantlyincreased111eperipheralleucocytecountandthepercentageofmononuclearleucocytesandneutrophilsweremarkedlyraisedTheactivityofNKcellandperitonealmacrophagewasremarkablystimulatedSBEsign

5、ificantlyincreasedtheimmunefunctionofCTXinducedimmunosuppressivemiceSinceSBEWasshowntoincreasetheimmunefunctionofCTXinducedimmunosuppressivemice，weexaminedwhetherSBEwouldinhibtthegrowthofHCCInpartlI，tocombineourpreviousf

6、indingsofHCC，theexperimentalmodelofhumanorthotopictransplantedHCCofnudemiceinvivowasusedtoinvestigatedinhibitoryeffectsofSBEOilbearingcancernudemiceWefocusedmainlyonthecellcyclechangesofhepatocellularcarcinomacellItWasfo

7、undthatthegrowthofhumanorthotopictransplantedHCCWasinhibitedbySBEn圮peripheralleucocytecountofnudemiceWasincreasedandAFPlevelofbloodserumwasdecreasedHepaticfunctionofbearingcancernudemiceWasamelioratedbySBEThehepatocellul

8、arcarcinomacellcountofG1phaseWassignificantlyincreasedinSBE30mg／kggroupTheprincipalmechanismmayrelate、Ⅳittlthechangesintheexpressionofp21，CyclinD1／CDK4，CyclinE／CDK2andPpRb11圮upregulationofp21anddownregulationofCyclinDI／C