RASSF1A基因在骨肉瘤中的表達及其臨床意義.pdf

1、華中科技大學(xué)碩士學(xué)位論文RASSF1A基因在骨肉瘤中的表達及其臨床意義姓名：劉之川申請學(xué)位級別：碩士專業(yè)：外科學(xué)（骨外科）指導(dǎo)教師：邵增務(wù)20090401華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 Expression of RASSF1A Gene in Osteosarcoma and its Clinical Significance Abstract Department of

2、 Orthopedics, Union hospital, Tongji medical college, Huazhong University of Science and Technology Master degree candidate：Liu Zhi-chuan Supervisor ：Prof. Shao Zeng-wu Objective: To investigate the expression

3、of turmor-supressing gene RASSF1A in osteosarcoma and its correlation with biological behavior. Methods: ⑴Culture MG-63 cell line and collect the third generation cell to RT-PCR detection. ⑵Collect 50 cases of Wu Han U

4、nion Hospital from September 2006 to September 2008 in which there are 30 osteosarcoma cases, 10 osteochondroma cases and 10 normal cases. ⑶Detect the expression of RASSF1A gene in osteosarcoma cell line MG-63、30 cases o

5、steosarcoma tissues、10 cases osteochondroma tissues and 10 cases normal bone tissues with the method of RT-PCR. Result: ① There was no expression of RASSF1A gene in osteosarcoma cell line MG-63. ② The expression nega

6、tive rate of RASSF1A was 43.33% in osteosarcoma tissues group; no expression absence of RASSF1A gene was detected in osteochondroma and normal bone tissues as control groups. The RASSF1A expression was significantly asso