多巴胺D1和谷氨酸NMDA受體參與調(diào)控異動(dòng)癥大鼠紋狀體△FosB蛋白的表達(dá).pdf

1、華中科技大學(xué)碩士學(xué)位論文多巴胺D1和谷氨酸NMDA受體參與調(diào)控異動(dòng)癥大鼠紋狀體△FosB蛋白的表達(dá)姓名：駱志堅(jiān)申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：王濤201005華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文4DopamineD1receptNmethylDaspartate(NMDA)receptinvolvedinregulationoftheexpressionof△FosBinthestriatumofratswithl

2、evodopainduceddyskinesiasMasterCidate:LuozhijianSupervis:Prof.WangTaoDepartmentofNeurologyUnionHospitalTongjiMedicalCollegeHuazhongUniversityofScienceTechnologyAbstractObjectiveToinvestigatetheeffectofdopamineD1dopamineD

3、2receptglutamateNMDAreceptonthebehaviexpressionof△FosBproteinintheratwithLevodopainducedDyskinesia.MethodsUnilaterallylesionedratmodelofParkinsonsdiseasewasestablishedbyusing6hydroxydopaminetheratmodelwastreatedwithlevod

4、opabenserazidedailyf28days.Allratsweredividedintosevengroups：nmalcontrolgroupPDgroupLIDgroupSCH23390groupMK801groupraclopridegroupnonLIDgroup.Thebehavialchangeswereobservedthesceofabnmalinvoluntarymovement(AIMsce)wereass

5、essedbyusingtheratAIMscale.Theexpressionof△FosBproteininstriatumweremeasuredcomparedineachgroupusingimmunohistochemistryassaywesternblotting.ResultsBothDopamineD1receptantagonistSCH23390NMDAreceptantagonistMK801attenuate

6、dabnmalbehaviofLIDratswhiledopamineD2receptantagonistraclopridehadnosignificanteffectonabnmalinvoluntarymovement△FosBinthelesionedstriatumofLIDratswasexpressedmethanthatofPDratsnonLIDratstheupregulationof△FosBinthelesion