抗肺癌治療性基因疫苗的純化工藝.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文抗肺癌治療性基因疫苗的純化工藝姓名：楊曉玲申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：牛勃20100530山西醫(yī)科大學(xué)碩士學(xué)位論文StudyonPurificationofTherapeuticDNAVaccineagainsthumanlungcancerAbstractBackgmund：A嘶_c觚cer(DNAVaccines)isare辯archhotspotinrecentyea璐athcme

2、鋤d曲mad，nismaildybyactiVatingtllepatient’sowniII】IlIlu∞sy毗miIIordcrt0∞mevet11epurpo∞oftumorrernoVal0rcontrolobjec晰e：nestudyes拓出lishedasolidfoulldacionofc00pera：tivec鋤Cerge∞tI坨r印yandh筋co璐idera【blesi盟ificanceilldeveloping鼢f

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7、嬲success伽lyconstr眥ted(2)111emtioofA2∞toA280、V勰18519211蛇contentofc訛lllarDNAw勰morettl觚93％11leendt10Xill獅dh0StDNAcontentswerelinle，lessth趾1OEU／mgH喊prote氓RNA孤1dgen0IIlicDNA毗ulldetec切bleTllere砌tshowsmatt11epurificationtectlno

8、logyisfe弱ible鋤ditlaysabaSisf0rl鶘escalepI叼d1Jctionof鋤ti蛐ortllerapeuticDNAvlccine觴well勰theothe娼Conclusion：IllttlisstudManew鑼peoflⅧ嗚c鋤certllerapeuticrccombiIlantDNAⅦccim(PVNYESO一1SSX2)w嬲叫fiedbySepharose6FFPlausmidSele鴨DEAES