豬水腫病大腸桿菌毒力基因突變菌株對(duì)豬的免疫效果研究.pdf

1、ⅱ2.2.3突變菌株的定植檢測(cè)..................................................272.2.4攻毒保護(hù)試驗(yàn).......................................................273.結(jié)果.......................................................................293.1微囊的制備

2、.............................................................293.1.1微囊形態(tài)與結(jié)構(gòu).....................................................293.1.2微囊的耐人工胃酸試驗(yàn)................................................303.1.3微囊的人工腸液溶出試驗(yàn).........

3、.....................................303.1.4微囊產(chǎn)品的貯存性能..................................................313.1.5微囊的安全性評(píng)價(jià)...................................................313.2免疫效果檢測(cè)........................................

4、...................313.2.1SLTIIeA蛋白的表達(dá)和純化...........................................313.2.2免疫效果測(cè)定.......................................................353.3突變菌株的定植檢測(cè).....................................................4

5、43.4攻毒保護(hù)實(shí)驗(yàn)...........................................................463.4.1大腸桿菌SLTIIe的提取及檢測(cè)........................................463.4.2臨床表現(xiàn)...........................................................473.4.3眼觀病變.......

6、....................................................473.4.4病理組織學(xué)變化.....................................................484.討論.......................................................................524.1微囊制備方法的選擇..........

7、...........................................524.2突變菌株在仔豬腸道內(nèi)的定植.............................................534.3黏膜免疫與口服疫苗.....................................................534.4仔豬口服突變菌株O139免疫效果評(píng)價(jià)........................

8、...............545.結(jié)論.......................................................................56致謝.........................................................................57參考文獻(xiàn).............................................