固定化酶的制備及其在海參飼料中的應(yīng)用研究.pdf

1、煙臺(tái)大學(xué)碩士學(xué)位論文固定化酶的制備及其在海參飼料中的應(yīng)用研究姓名：李向陽(yáng)申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：化學(xué)工程指導(dǎo)教師：徐世艾2011-04煙 臺(tái) 大 學(xué) 碩 士 學(xué) 位 論 文 煙 臺(tái) 大 學(xué) 碩 士 學(xué) 位 論 文 II Abstract This thesis focuses on the application research of improving sea cucumber breeding baits digestion a

2、nd absorption by adopting enzyme engineering technology. At present, the digestive enzymes are widespread applied in the animal feed, which can promote digestion and absorption of nutrients, improve the growth rate and f

3、eed conversion rate of animals, and improve the animal health, and it also can cut down nitrogen and phosphate emissions of breeding, and protect breeding ecological environment. Therefore, the feeding enzyme preparation

4、 plays dual role in increasing economic benefits and protect the ecological environment in the modern aquaculture industry, and the relevant research on digestive enzyme is more and more concerned by people. In this work

5、, based on the growing environment characteristics of sea cucumber, the main digestive enzymes α-amylase and acidic pepsin were embedding immobilized, which exists in sea cucumber intestinal canal as research object, and

6、 then them were used to improve the digestive absorption of sea cucumber baits.Their enzymology character and immobilized condition optimizing are studied, and then, the related properties of immobilized enzyme and its a

7、pplication on sea cucumber feed are researched. The main research contents and conclusions are as follows: (1) Free enzymology character study. It involves the optimum reaction temperature and pH. Results show that: when

8、 the fungus α-amylase liquid is diluted 1,000 times, the optimum value of temperature and pH for reaction are 60 ℃ and 5.3. When the acidic pepsin powder is diluted 1,500 times, the optimum value of temperature and pH fo

9、r reaction are 58 ℃ and 2.6. (2) Immobilized preparation conditions optimization research. Through single factor and orthogonal test, the influencing factors of immobilized preparation conditions is discussed, such as s

10、odium alginate concentration, calcium chloride concentration, plus enzyme quantity, immobilized time. Then, the optimal preparation process is obtained. The results of study proved the activity of the immobilized α-amyla

11、se is the highest when the related parameters are as follows: 2.5% sodium alginate, 15% enzyme amount, 2% CaCl2 and immobilized time 1 h. And the activity of immobilized acidic stomach protease is the best when the relat