非同步移植胚胎的著床及SNAIL和COX-2在著床位點的表達.pdf

1、東北農(nóng)業(yè)大學碩士學位論文非同步移植胚胎的著床及SNAIL和COX2在著床位點的表達姓名：李世杰申請學位級別：碩士專業(yè)：基礎獸醫(yī)學指導教師：楊增明20040501東北農(nóng)業(yè)大學農(nóng)學碩士學位論文THEIMPLANTATIoNoFASYNCHRONoUSLyTRANSFERREDEMBRYoSA^『DTHEEXPRESSl0N0FSNAILANDCoX2ATTHESlTEoFIMPLANTALTl0NABSTRACTBlastocystimpl

2、antationisakeystepforSUCCESSfulestablishmentofpregnancyduringmammalianreproductionSuccessfulimplantationisdependentupontheinteractionbetweentheblastocystandtheuterusThemostfundamentalfeatureofthisprocessiSthesynchronized

3、developmentoftheembryototheblastocyststageanddifferentiationoftheuterustothereceptivestateTheexpressionofimplantationrelatedmoleculeshasnotbeenexaminedafterasynchronousembryotransferInthisstudy，fourgroupshadbeendesigned，

4、including(A)Zygotesand8cellstageembryosweretransferredseparatelyintotheoviductsofthesamerecipientOnday1ofpseudopregnancy；(B)Embryosofzygotesandblastocystsweretransferredseparatelyintotheoviductsofthesamerecipientonday1of

5、pseudopregnancy；(C)Morulaweretransferredintotheoviductsoftherecipientonday1ofpseudopregnancy；(D)Blastocystsweretransferredintotheoviductsoftherecipientonday1ofpseudopregnancyEmbryoimplantationwasexaminedatI6：00and20：00of

6、day4，andat8：00ofday5byintravenousinjectionofChicagobluedyeTheexpressionofbothSNAILandCOX2atimplantationsiteswasexaminedbyimmunohistochemistryInthisstudy，theembryosasynchronouslytransferredcouldimplantinalloffourgroups，On

7、day5posttransferthesizeofimplantedembryosbetweeneachexpedmentalgroupsWasdifferent，thedifferenceingroupAandbetweenzygoteandmorulatransferredinsamedayWasverysignificant(PO01)，thegroupBthatthesizeofimplantedzygoteswassignif

8、icantlysmallerthanthatofblastocysts(P005)Themorphologiesofimplantedembryosweredifferentbetweeneachgroup，havingtheembryosfromattachedblastocyststoeggcylinder，TheimmunostainingofbothSNAILandCOX2wasalsodifferentineachgroupW

9、hentheembryosweretransferredat8cell，morulaorblastocyststage，theimmanostainingforSNAILandCOX2wasstrongerthanthattransferredatzygotestageTheseresultssuggestthattheembryostransferredatadvancedstagecouldimplantpriortoyounger

10、embryosandimplantation—relatedmoleculeswerealsoexpressedatimplantationsitesofmouseuteruswhentheadvancedembryoswereimplantedPostgraduate：LiShijieSpecialty：DeveIopmentalBiologySupervisor：ProfessorYangZengMingKeywords：mouse