多糖提取外文翻譯--應(yīng)用均勻設(shè)計優(yōu)化提取絞股藍(lán)多糖工藝的研究（英文）

1、Optimization of polysaccharides extraction from Gynostemma pentaphyllum Makino using Uniform DesignEun Hye Lee , Mi-Sun Yum , Min-Hee Jeong Department of Pediatrics, College of Medicine, Kyung Hee University, Seoul, Repu

2、blic of Korea Received 12 April 2006; received in revised form 5 August 2006; accepted 5 October 2006 Available online 27 November 2006AbstractUniform Design was used to optimize the effects of processing parameters of e

3、xtraction from Gynostemma pentaphyllum Makino on the yield of polysaccharides. Four independent variables such as extraction time (min), solid:liquid ratio (g/ml), immersing time (min), and extraction temperature (?C) we

4、re study. The optimal conditions were determined and Three-dimensional response surfaces were plotted from the mathematical models. The t-value and p-value indicated that both immersing time and water extraction time (X2

5、X4) had interaction effects on the response value, followed by the linear terms of water extraction time (X4), and the interaction effects of immersing time and water extraction temperature(X2X3). Considering the efficie

6、ncy, the optimum conditions of polysaccharides extrac- tion were liquid:solid ratio of 1:67, immersing time of 10 min, water extraction temperature of 95 ?C, and water extraction time of 15 min. Under optimized condition

7、s, the experimental yield 11.29% agreed closely with the predicted yield. Therefore, application of Uniform Design in the extraction of polysaccharides from Gynostemma pentaphyllum Makino dramatically reduced extraction

8、time. ? 2006 Elsevier Ltd. All rights reserved.Keywords: Gynostemma pentaphyllum Makino; Polysaccharide; Extraction; Uniform Design1. IntroductionIt is well accepted that carbohydrates can serve as struc- tural component

9、s and energy source of the cell. More inter- estingly, their highly complex structure allows very specific interactions so that these biomolecules are involved in a variety of molecular recognition processes in intercell

10、ular communication and signal transduction such as cell adhe- sion, differentiation, development, regulation, etc. (Varki, 1993). For these reasons, great interest arose on polysac- charides-based pharmaceuticals and on

11、the development of the analysis and extraction of polysaccharides. Gynostemma pentaphyllum Makino is a well known edi- ble and medicinal plant in oriental countries (Hu, Chen, Tsunematu, Shigenobu, Triveni, Shamala, w

12、ater extraction time, 120 min. Fig. 2 shows the yield of polysaccharides increased with elevating sol- id:liquid ratio.Table 1 Independent variables of the process and their corresponding levelsIndependent variables Leve

13、ls1 2 3 4 5 6 7 8 9 10 11 12 13Solid:liquid ratio (g/ml) 1:10 1:15 1:20 1:25 1:30 1:35 1:40 1:45 1:50 1:55 1:60 1:65 1:70 Immersing time (min) 0 10 20 30 40 50 60 70 80 90 100 110 120 Water extraction temperature (?C) 35

14、 40 45 50 55 60 65 70 75 80 85 90 95 Water extraction time (min) 15 30 45 60 75 90 105 120 135 150 165 180 195Table 2 Uniform Design with the observed responses and predicted valuesTreat Variable levels Experimental Ye P

15、redicted Y Ye?YX1 X2 X3 X41 1 5 9 11 7.13000 7.36332 ?0.23332 2 2 10 4 8 5.30000 5.69461 ?0.39461 3 3 1 13 5 4.89000 4.79510 0.09490 4 4 6 8 2 6.89000 6.33304 0.55696 5 5 11 3 13 5.05000 4.51014 0.53986 6 6 2 12 10 7.490

16、00 7.21565 0.27435 7 7 7 7 7 6.99000 7.06128 ?0.07128 8 8 12 2 4 6.31000 6.60065 ?0.29065 9 9 3 11 1 5.67000 6.17014 ?0.50014 10 10 8 6 12 5.20000 5.56554 ?0.36554 11 11 13 1 9 3.39000 3.41260 ?0.02260 12 12 4 10 6 6.550

17、00 6.58711 ?0.03711 13 13 9 5 3 8.09000 7.64082 0.44918Fig. 1. Effect of immersing time on the polysaccharides yield in water extraction.Fig. 2. Effect of solid:liquid ratio on the polysaccharides yield in water extracti