石油烴污染土壤微生物多樣性分析及多環(huán)芳烴降解菌研究.pdf

1、新疆農(nóng)業(yè)大學碩士學位論文石油烴污染土壤微生物多樣性分析及多環(huán)芳烴降解菌研究姓名：何晶晶申請學位級別：碩士專業(yè)：環(huán)境科學指導(dǎo)教師：王純利李慧20080501ABSTRACTPetroleumhydrocarbonsarewidespreadindustrialpollutantsthatarereleasedintotheenvironmentbytransportingandstoringofcrudeoil，refiningofpet

2、roleum，accidentalleakingandpetroleumcontainingwastewaterirrigation，etcHowevermicrobialdiversityimpactsofpetroleumpollutiononsoilbyARDRAhavebeenscarcelystudiedConventionalculturemethodandmodernmoleculartechniques16srDNApo

3、lymerasechainreaction(PCR)Amplified—rDNARestrictionAnalysis(ARDRA)werecombinedtoevaluatetheeffectsoflong—termpetroleum—containingwastewaterirrigationonbacterialcommunitystructuresanddiversitiesinsoilsinShenfuirrigationar

4、ea，thelargestareairrigatedbyoilcontainingwastewaterformorethan50yrinnortheasternChinaTheresultsshowedthatsoiltotalpetroleumhydrocarbon(TPH)andpolycyclicaromatichydrocarbons(PAHs)concentrationdeclinedalongthegradientofthe

5、irrigationchannelfromup—todownstreamConstructionof16srDNAgenomiclibraryhadpopulationdiversityProteobacteriaandActinobacteriaweredominantinShenfuirrigationareaAlsocontainedmanykindsofsurvivedbacteriabutunculturedThreepyre

6、nedegradingstrainsandonefluoranthenedegradingstrainwereisolatedbyselectiveenrichmentcultureandscreeningmodelofanoverlayerfromhi【曲concentrationdieseloil—stressedsoilinlaboratoryTheeffectofCOmetabolicinincreasingthedegrada